Restriction map for pF1KB0937
 (Linear) (Six-base) MAPSORT of: KIBB0937.seq  Check: 3536  from: 1  to: 480



KIBB0937.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AfeI AGC'GCT

Cuts at:      0      82     480 
   Size:         82     398
 
AlwNI CAG_nnn'CTG

Cuts at:      0     360     480 
   Size:        360     120
 
AvaI C'yCGr_G

Cuts at:      0      64     138     480 
   Size:         64      74     342

  Fragments arranged by size: 

                 342      74      64
 
BanI G'GyrC_C

Cuts at:      0      46     480 
   Size:         46     434
 
BanII G_rGCy'C

Cuts at:      0      13      65     480 
   Size:         13      52     415

  Fragments arranged by size: 

                 415      52      13
 
BbvCI CC'TCA_GC

Cuts at:      0     421     480 
   Size:        421      59
 
BglI GCCn_nnn'nGGC

Cuts at:      0     259     480 
   Size:        259     221
 
BlpI GC'TnA_GC

Cuts at:      0     301     480 
   Size:        301     179
 
Bme1580I G_kGCm'C

Cuts at:      0      49     480 
   Size:         49     431
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     231     480 
   Size:        231     249
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27     480 
   Size:         27     453
 
Bpu10I CC'TnA_GC

Cuts at:      0     421     480 
   Size:        421      59
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     480 
   Size:        304     176
 
BsaAI yAC'GTr

Cuts at:      0     405     480 
   Size:        405      75
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     418     442     448     472     480 
   Size:        418      24       6      24       8

  Fragments arranged by size: 

                 418      24      24       8       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19      70      73     199     480 
   Size:         19      51       3     126     281

  Fragments arranged by size: 

                 281     126      51      19       3
 
BseYI C'CCAG_C

Cuts at:      0     166     480 
   Size:        166     314
 
BsiEI CG_ry'CG

Cuts at:      0      90     480 
   Size:         90     390
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     480 
   Size:         65     415
 
Bsp1286I G_dGCh'C

Cuts at:      0      13      49      65     480 
   Size:         13      36      16     415

  Fragments arranged by size: 

                 415      36      16      13
 
BssHII G'CGCG_C

Cuts at:      0     170     480 
   Size:        170     310
 
BssSI C'ACGA_G

Cuts at:      0     412     480 
   Size:        412      68
 
BstYI r'GATC_y

Cuts at:      0     308     480 
   Size:        308     172
 
BtgI C'CryG_G

Cuts at:      0      32     480 
   Size:         32     448
 
EaeI y'GGCC_r

Cuts at:      0      87     209     326     480 
   Size:         87     122     117     154

  Fragments arranged by size: 

                 154     122     117      87
 
EagI C'GGCC_G

Cuts at:      0      87     480 
   Size:         87     393
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     459     480 
   Size:        459      21
 
EcoICRI GAG'CTC

Cuts at:      0      63     480 
   Size:         63     417
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     352     480 
   Size:        352     128
 
EcoO109I rG'GnC_Cy

Cuts at:      0     357     480 
   Size:        357     123
 
FspI TGC'GCA

Cuts at:      0     268     480 
   Size:        268     212
 
HaeII r_GCGC'y

Cuts at:      0      84     102     228     265     420     480 
   Size:         84      18     126      37     155      60

  Fragments arranged by size: 

                 155     126      84      60      37      18
 
MscI TGG'CCA

Cuts at:      0     211     480 
   Size:        211     269
 
MspA1I CmG'CkG

Cuts at:      0     395     480 
   Size:        395      85
 
PpuMI rG'GwC_Cy

Cuts at:      0     357     480 
   Size:        357     123
 
PstI C_TGCA'G

Cuts at:      0     194     365     400     480 
   Size:        194     171      35      80

  Fragments arranged by size: 

                 194     171      80      35
 
PvuII CAG'CTG

Cuts at:      0     395     480 
   Size:        395      85
 
SacI G_AGCT'C

Cuts at:      0      65     480 
   Size:         65     415
 
SexAI A'CCwGG_T

Cuts at:      0     353     480 
   Size:        353     127
 
SfcI C'TryA_G

Cuts at:      0     102     190     361     396     480 
   Size:        102      88     171      35      84

  Fragments arranged by size: 

                 171     102      88      84      35
 
SmaI CCC'GGG

Cuts at:      0     140     480 
   Size:        140     340
 
SmlI C'TyrA_G

Cuts at:      0      64     319     480 
   Size:         64     255     161

  Fragments arranged by size: 

                 255     161      64
 
StuI AGG'CCT

Cuts at:      0     253     480 
   Size:        253     227
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     250     480 
   Size:        250     230
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     442     474     480 
   Size:        442      32       6

  Fragments arranged by size: 

                 442      32       6
 
XhoI C'TCGA_G

Cuts at:      0      64     480 
   Size:         64     416
 
XmaI C'CCGG_G

Cuts at:      0     138     480 
   Size:        138     342
 

 Enzymes that do cut:

     AfeI    AlwNI     AvaI     BanI    BanII    BbvCI     BglI     BlpI
 Bme1580I     BmrI     BplI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I   BssHII    BssSI    BstYI     BtgI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I     FspI    HaeII
     MscI   MspA1I    PpuMI     PstI    PvuII     SacI    SexAI     SfcI
     SmaI     SmlI     StuI    TaqII     TstI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI     BpmI
     BsaI    BsaBI    BsaHI    BsaWI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EarI  Eco57MI    EcoRI    EcoRV
     FalI     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
  Tth111I     XbaI     XcmI     XmnI     ZraI
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