Restriction map for pF1KB5887
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspHI       AspI        
AvaII       AviII       AvrII       BamHI       BanII       
BbrPI       BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRI       EcoT22I     FauI        
FbaI        FseI        FspI        HgaI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MroI        MslI        MunI        MvnI        NaeI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          312  GTCTAC               GT^[AC][GT]AC
AciI          183  GCGG                 CCGC(-3/-1)
AcyI         1325  GGCGCC               G[GA]^CG[CT]C
AhdI         1397  GACATCTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1183  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1397  GACATCTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          462  CCCGAG               C^[CT]CG[GA]G
BalI          517  TGGCCA               TGG^CCA
BanI         1325  GGCGCC               G^G[CT][GA]CC
BbeI         1325  GGCGCC               GGCGC^C
BglII        1236  AGATCT               A^GATCT
BsaHI        1325  GGCGCC               G[GA]^CG[CT]C
BsgI          592  GTGCAG               GTGCAG(16/14)
BsmI         1245  GAATGC               GAATGC(1/-1)
BsoBI         462  CCCGAG               C^[CT]CG[GA]G
BssSI         143  CACGAG               CACGAG(-5/-1)
BstBI         710  TTCGAA               TT^CGAA
BstEII        583  GGTGACC              G^GT[ACGT]ACC
BstF5I        779  CATCC                GGATG(2/0)
BstPI         583  GGTGACC              G^GT[ACGT]ACC
CfoI         1326  GCGC                 GCG^C
Cfr13I        693  GGGCC                G^G[ACGT]CC
DsaI          782  CCATGG               C^C[GA][CT]GG
EaeI          517  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1397  GACATCTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       583  GGTGACC              G^GT[ACGT]ACC
EcoRV        1194  GATATC               GAT^ATC
FokI          779  CATCC                GGATG(9/13)
HaeII        1325  GGCGCC               [GA]GCGC^[CT]
HapII         696  CCGG                 C^CGG
HhaI         1326  GCGC                 GCG^C
Hin1I        1325  GGCGCC               G[GA]^CG[CT]C
HinP1I       1326  GCGC                 G^CGC
HindIII      1342  AAGCTT               A^AGCTT
HpaI          835  GTTAAC               GTT^AAC
HpaII         696  CCGG                 C^CGG
KasI         1325  GGCGCC               G^GCGCC
MluNI         517  TGGCCA               TGG^CCA
MscI          517  TGGCCA               TGG^CCA
MspA1I        203  CAGCTG               C[AC]G^C[GT]G
MspI          696  CCGG                 C^CGG
MwoI         1317  GCAGCTAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI         1325  GGCGCC               GG^CGCC
NcoI          782  CCATGG               C^CATGG
NspV          710  TTCGAA               TT^CGAA
PshAI        1435  GACAAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           41  CTGCAG               CTGCA^G
PvuII         203  CAGCTG               CAG^CTG
SapI         1312  GAAGAGC              GCTCTTC(1/4)
Sau96I        693  GGGCC                G^G[ACGT]CC
ScaI         1306  AGTACT               AGT^ACT
SfoI         1325  GGCGCC               GGC^GCC
SfuI          710  TTCGAA               TT^CGAA
TaqI          711  TCGA                 T^CGA
TthHB8I       711  TCGA                 T^CGA
XmnI         1183  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwI           22  GGATC                GGATC(4/5)
              803  GGATC               
BbsI          242  GAAGAC               GAAGAC(2/6)
              940  GAAGAC              
BpuAI         242  GAAGAC               GAAGAC(2/6)
              940  GAAGAC              
BsiYI         383  CCTTAGACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1329  CCTGCAAGAGG         
BslI          383  CCTTAGACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1329  CCTGCAAGAGG         
BsmAI         227  GAGAC                GTCTC(1/5)
              400  GAGAC               
BspMI          39  ACCTGC               ACCTGC(4/8)
              432  GCAGGT              
BssKI         647  CCTGG                ^CC[ACGT]GG
              973  CCAGG               
BstNI         647  CCTGG                CC^[AT]GG
              973  CCAGG               
Cac8I         849  GCTTGC               GC[ACGT]^[ACGT]GC
             1328  GCCTGC              
EarI           73  GAAGAG               CTCTTC(1/4)
             1312  GAAGAG              
EcoNI         383  CCTTAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1329  CCTGCAAGAGG         
EcoRII        647  CCTGG                ^CC[AT]GG
              973  CCAGG               
EcoT14I       782  CCATGG               C^C[AT][AT]GG
              946  CCTTGG              
HincII        835  GTTAAC               GT[CT]^[GA]AC
             1432  GTTGAC              
HindII        835  GTTAAC               GT[CT]^[GA]AC
             1432  GTTGAC              
Ksp632I        73  GAAGAG               CTCTTC(1/4)
             1312  GAAGAG              
MaeIII        584  GTGAC                ^GT[ACGT]AC
             1405  GTCAC               
MvaI          647  CCTGG                CC^[AT]GG
              973  CCAGG               
NspI          405  ACATGC               [GA]CATG^[CT]
              957  ACATGC              
ScrFI         647  CCTGG                CC^[ACGT]GG
              973  CCAGG               
SfaNI         724  GATGC                GCATC(5/9)
              130  GCATC               
SfcI           41  CTGCAG               C^T[GA][CT]AG
              206  CTGTAG              
SmlI         1042  CTTGAG               C^T[CT][GA]AG
             1219  CTTGAG              
StyI          782  CCATGG               C^C[AT][AT]GG
              946  CCTTGG              
TfiI          699  GAATC                G^A[AT]TC
              718  GAATC               
Tsp45I        584  GTGAC                ^GT[GC]AC
             1405  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbvI        
BciVI       BfaI        BsaJI       BseRI       BsmFI       
BsrI        BstYI       DdeI        DpnII       Fnu4HI      
HaeIII      HinfI       HphI        ItaI        MaeI        
MboI        MboII       MflI        MnlI        MseI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      SspI        Tru9I       TseI        Tsp509I     
TspRI       XhoII       
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