Restriction map for pF1KE1038
 (Linear) (Six-base) MAPSORT of: KIEE1038.seq  Check: 1283  from: 1  to: 2016



KIEE1038.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      24    2016 
   Size:         24    1992
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1574    1607    2016 
   Size:       1574      33     409

  Fragments arranged by size: 

                1574     409      33
 
AlwNI CAG_nnn'CTG

Cuts at:      0     262    2016 
   Size:        262    1754
 
ApoI r'AATT_y

Cuts at:      0     242     692    1022    1150    1266    1424    2016 
   Size:        242     450     330     128     116     158     592

  Fragments arranged by size: 

                 592     450     330     242     158     128     116
 
AseI AT'TA_AT

Cuts at:      0    1001    2016 
   Size:       1001    1015
 
AvaI C'yCGr_G

Cuts at:      0     184    2016 
   Size:        184    1832
 
BanI G'GyrC_C

Cuts at:      0    1337    2016 
   Size:       1337     679
 
BanII G_rGCy'C

Cuts at:      0     962    2016 
   Size:        962    1054
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      61     620    1088    2016 
   Size:         61     559     468     928

  Fragments arranged by size: 

                 928     559     468      61
 
BclI T'GATC_A

Cuts at:      0     843    1500    2016 
   Size:        843     657     516

  Fragments arranged by size: 

                 843     657     516
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1394    2016 
   Size:       1394     622
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     220    2016 
   Size:        220    1796
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     629    1589    1858    2016 
   Size:        629     960     269     158

  Fragments arranged by size: 

                 960     629     269     158
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63    1351    2016 
   Size:         63    1288     665

  Fragments arranged by size: 

                1288     665      63
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1604    1634    1673    1703    1831    1861    2016 
   Size:       1604      30      39      30     128      30     155

  Fragments arranged by size: 

                1604     155     128      39      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     728    2016 
   Size:        728    1288
 
BseYI C'CCAG_C

Cuts at:      0     935    2016 
   Size:        935    1081
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1888    1976    2016 
   Size:       1888      88      40

  Fragments arranged by size: 

                1888      88      40
 
BsiHKAI G_wGCw'C

Cuts at:      0     515    2016 
   Size:        515    1501
 
Bsp1286I G_dGCh'C

Cuts at:      0     515     962    2016 
   Size:        515     447    1054

  Fragments arranged by size: 

                1054     515     447
 
BspHI T'CATG_A

Cuts at:      0     117    1503    1902    2016 
   Size:        117    1386     399     114

  Fragments arranged by size: 

                1386     399     117     114
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      24    1998    2016 
   Size:         24    1974      18

  Fragments arranged by size: 

                1974      24      18
 
BstYI r'GATC_y

Cuts at:      0       3     529    2016 
   Size:          3     526    1487

  Fragments arranged by size: 

                1487     526       3
 
Bsu36I CC'TnA_GG

Cuts at:      0     233    2016 
   Size:        233    1783
 
BtsI GCAGTG_nn'

Cuts at:      0     173    2016 
   Size:        173    1843
 
DraI TTT'AAA

Cuts at:      0      14    1695    1817    1886    2016 
   Size:         14    1681     122      69     130

  Fragments arranged by size: 

                1681     130     122      69      14
 
EarI CTCTTCn'nnn_

Cuts at:      0    1946    2016 
   Size:       1946      70
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1438    2016 
   Size:       1438     578
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     629    1574    1589    1607    1858    2016 
   Size:        629     945      15      18     251     158

  Fragments arranged by size: 

                 945     629     251     158      18      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1607    2016 
   Size:       1607     409
 
EcoRI G'AATT_C

Cuts at:      0    1424    2016 
   Size:       1424     592
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     787    1753    2016 
   Size:        787     966     263

  Fragments arranged by size: 

                 966     787     263
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     206     238    1288    1320    2016 
   Size:        206      32    1050      32     696

  Fragments arranged by size: 

                1050     696     206      32      32
 
MfeI C'AATT_G

Cuts at:      0    1379    2016 
   Size:       1379     637
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1025    2016 
   Size:       1025     991
 
MslI CAynn'nnrTG

Cuts at:      0     395    2016 
   Size:        395    1621
 
MspA1I CmG'CkG

Cuts at:      0    1292    2016 
   Size:       1292     724
 
NdeI CA'TA_TG

Cuts at:      0    1410    2016 
   Size:       1410     606
 
PfoI T'CCnGG_A

Cuts at:      0     558    2016 
   Size:        558    1458
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     818     850    2016 
   Size:        818      32    1166

  Fragments arranged by size: 

                1166     818      32
 
PstI C_TGCA'G

Cuts at:      0      35     258    2016 
   Size:         35     223    1758

  Fragments arranged by size: 

                1758     223      35
 
PvuII CAG'CTG

Cuts at:      0    1292    2016 
   Size:       1292     724
 
SfcI C'TryA_G

Cuts at:      0      31     254    1671    1966    2016 
   Size:         31     223    1417     295      50

  Fragments arranged by size: 

                1417     295     223      50      31
 
SmaI CCC'GGG

Cuts at:      0     186    2016 
   Size:        186    1830
 
SmlI C'TyrA_G

Cuts at:      0      78    1366    2016 
   Size:         78    1288     650

  Fragments arranged by size: 

                1288     650      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     739    1557    1844    2016 
   Size:        739     818     287     172

  Fragments arranged by size: 

                 818     739     287     172
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     521     553    1604    1636    2016 
   Size:        521      32    1051      32     380

  Fragments arranged by size: 

                1051     521     380      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1647    1947    2016 
   Size:       1647     300      69

  Fragments arranged by size: 

                1647     300      69
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     683    2016 
   Size:        683    1333
 
XmaI C'CCGG_G

Cuts at:      0     184    2016 
   Size:        184    1832
 
XmnI GAAnn'nnTTC

Cuts at:      0     830    2016 
   Size:        830    1186
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApoI     AseI     AvaI     BanI    BanII
     BbsI     BclI     BglI     BmrI     BpmI    BpuEI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BstYI   Bsu36I
     BtsI     DraI     EarI     EciI  Eco57MI    EcoNI    EcoRI     FalI
    Hin4I     MfeI     MmeI     MslI   MspA1I     NdeI     PfoI     PsrI
     PstI    PvuII     SfcI     SmaI     SmlI    TaqII     TstI     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI  EcoICRI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I     XhoI
     ZraI
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