Restriction map for pF1KB5232
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       AccI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        KspI        MfeI        MflI        MluI        
MslI        MunI        NaeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII           6  CGCG                 CG^CG
AccIII       1266  TCCGGA               T^CCGGA
AclI         1135  AACGTT               AA^CGTT
AlwI         1200  GATCC                GGATC(4/5)
AocI         1498  CCTCAGG              CC^T[ACGT]AGG
ApaI          136  GGGCCC               GGGCC^C
Asp700I       469  GAACGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1053  GAGCAC               G[AT]GC[AT]^C
AvaI         1064  CCCGGG               C^[CT]CG[GA]G
BfaI          643  CTAG                 C^TAG
BglI           31  GCCCTCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1434  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1434  GCTGAGC              GC^T[ACGT]AGC
BsaBI         802  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1266  TCCGGA               [AT]^CCGG[AT]
BseAI        1266  TCCGGA               T^CCGGA
BsiHKAI      1053  GAGCAC               G[AT]GC[AT]^C
BsmFI        1229  GGGAC                GGGAC(10/14)
BsmI          473  GCATTC               GAATGC(1/-1)
BsoBI        1064  CCCGGG               C^[CT]CG[GA]G
BspEI        1266  TCCGGA               T^CCGGA
BspMI        1181  ACCTGC               ACCTGC(4/8)
BstUI           6  CGCG                 CG^CG
BstXI         315  CCAAAACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1498  CCTCAGG              CC^T[ACGT]AGG
CelII        1434  GCTGAGC              GC^T[ACGT]AGC
DraIII       1236  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          700  CCATGG               C^C[GA][CT]GG
Eco81I       1498  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1182  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI          643  CTAG                 C^TAG
MaeII        1136  ACGT                 A^CGT
MamI          802  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1266  TCCGGA               T^CCGGA
MvnI            6  CGCG                 CG^CG
MwoI           31  GCCCTCTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          700  CCATGG               C^CATGG
NdeI          778  CATATG               CA^TATG
PpuMI        1229  GGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I     1135  AACGTT               AA^CGTT
SapI          456  GAAGAGC              GCTCTTC(1/4)
SfaNI         835  GATGC                GCATC(5/9)
SmaI         1064  CCCGGG               CCC^GGG
SphI         1339  GCATGC               GCATG^C
TaqI          182  TCGA                 T^CGA
TfiI          807  GAATC                G^A[AT]TC
TthHB8I       182  TCGA                 T^CGA
XmaCI        1064  CCCGGG               C^CCGGG
XmaI         1064  CCCGGG               C^CCGGG
XmnI          469  GAACGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I       1175  GGTACC               G^GTACC
             1192  GGTACC              
AcsI          712  AAATTC               [GA]^AATT[CT]
             1402  AAATTT              
AcyI          113  GGCGCC               G[GA]^CG[CT]C
              143  GGCGCC              
ApoI          712  AAATTC               [GA]^AATT[CT]
             1402  AAATTT              
Asp718I      1175  GGTACC               G^GTACC
             1192  GGTACC              
BbeI          113  GGCGCC               GGCGC^C
              143  GGCGCC              
BpmI          196  CTGGAG               CTGGAG(16/14)
             1279  CTGGAG              
BsaHI         113  GGCGCC               G[GA]^CG[CT]C
              143  GGCGCC              
BseRI         481  GAGGAG               GAGGAG(10/8)
              514  GAGGAG              
BsgI         1222  CTGCAC               GTGCAG(16/14)
             1105  GTGCAG              
BsmAI         824  GAGAC                GTCTC(1/5)
              940  GAGAC               
DpnII         549  GATC                 ^GATC
             1200  GATC                
FauI          129  CCCGC                CCCGC(4/6)
             1335  GCGGG               
Hin1I         113  GGCGCC               G[GA]^CG[CT]C
              143  GGCGCC              
KasI          113  GGCGCC               G^GCGCC
              143  GGCGCC              
KpnI         1175  GGTACC               GGTAC^C
             1192  GGTACC              
MboI          549  GATC                 ^GATC
             1200  GATC                
MspA1I        205  CAGCTG               C[AC]G^C[GT]G
              628  CAGCTG              
NarI          113  GGCGCC               GG^CGCC
              143  GGCGCC              
NdeII         549  GATC                 ^GATC
             1200  GATC                
NspI          676  ACATGC               [GA]CATG^[CT]
             1339  GCATGC              
PflMI        1235  CCACAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1272  CCATTTTCTGG         
PleI           57  GACTC                GAGTC(4/5)
             1069  GAGTC               
PvuII         205  CAGCTG               CAG^CTG
              628  CAGCTG              
Sau3AI        549  GATC                 ^GATC
             1200  GATC                
SfoI          113  GGCGCC               GGC^GCC
              143  GGCGCC              
Van91I       1235  CCACAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1272  CCATTTTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboII       MluNI       MnlI        
MscI        MseI        MspI        MvaI        NciI        
NlaIII      NlaIV       PstI        RsaI        Sau96I      
ScrFI       SfcI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
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