Restriction map for pF1KB5232
 (Linear) (Six-base) MAPSORT of: KIBB5232.seq  Check: 5242  from: 1  to: 1506



KIBB5232.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1190    1506 
   Size:       1190     316
 
Acc65I G'GTAC_C

Cuts at:      0    1175    1192    1506 
   Size:       1175      17     314

  Fragments arranged by size: 

                1175     314      17
 
AclI AA'CG_TT

Cuts at:      0    1136    1506 
   Size:       1136     370
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     655     726    1395    1462    1506 
   Size:        521     134      71     669      67      44

  Fragments arranged by size: 

                 669     521     134      71      67      44
 
AlwNI CAG_nnn'CTG

Cuts at:      0     630     633     749    1506 
   Size:        630       3     116     757

  Fragments arranged by size: 

                 757     630     116       3
 
ApaI G_GGCC'C

Cuts at:      0     140    1506 
   Size:        140    1366
 
ApoI r'AATT_y

Cuts at:      0     712    1402    1506 
   Size:        712     690     104

  Fragments arranged by size: 

                 712     690     104
 
AvaI C'yCGr_G

Cuts at:      0    1064    1506 
   Size:       1064     442
 
BanI G'GyrC_C

Cuts at:      0     113     143    1175    1192    1506 
   Size:        113      30    1032      17     314

  Fragments arranged by size: 

                1032     314     113      30      17
 
BanII G_rGCy'C

Cuts at:      0      19     140     969    1506 
   Size:         19     121     829     537

  Fragments arranged by size: 

                 829     537     121      19
 
BbeI G_GCGC'C

Cuts at:      0     117     147    1506 
   Size:        117      30    1359

  Fragments arranged by size: 

                1359     117      30
 
BglI GCCn_nnn'nGGC

Cuts at:      0      37    1506 
   Size:         37    1469
 
BlpI GC'TnA_GC

Cuts at:      0    1435    1506 
   Size:       1435      71
 
Bme1580I G_kGCm'C

Cuts at:      0      73     140    1506 
   Size:         73      67    1366

  Fragments arranged by size: 

                1366      73      67
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      76    1465    1506 
   Size:         76    1389      41

  Fragments arranged by size: 

                1389      76      41
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217    1300    1506 
   Size:        217    1083     206

  Fragments arranged by size: 

                1083     217     206
 
BsaBI GATnn'nnATC

Cuts at:      0     806    1506 
   Size:        806     700
 
BsaHI Gr'CG_yC

Cuts at:      0     114     144    1506 
   Size:        114      30    1362

  Fragments arranged by size: 

                1362     114      30
 
BsaWI w'CCGG_w

Cuts at:      0    1266    1506 
   Size:       1266     240
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     496     529    1506 
   Size:        496      33     977

  Fragments arranged by size: 

                 977     496      33
 
BseYI C'CCAG_C

Cuts at:      0    1171    1506 
   Size:       1171     335
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1126    1207    1506 
   Size:       1126      81     299

  Fragments arranged by size: 

                1126     299      81
 
BsiHKAI G_wGCw'C

Cuts at:      0    1057    1506 
   Size:       1057     449
 
BsmI GAATG_Cn'

Cuts at:      0     473    1506 
   Size:        473    1033
 
Bsp1286I G_dGCh'C

Cuts at:      0      19      73     140     969    1057    1506 
   Size:         19      54      67     829      88     449

  Fragments arranged by size: 

                 829     449      88      67      54      19
 
BspEI T'CCGG_A

Cuts at:      0    1266    1506 
   Size:       1266     240
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1190    1506 
   Size:       1190     316
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     322    1506 
   Size:        322    1184
 
Bsu36I CC'TnA_GG

Cuts at:      0    1499    1506 
   Size:       1499       7
 
BtgI C'CryG_G

Cuts at:      0     700    1506 
   Size:        700     806
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      25     975    1506 
   Size:         25     950     531

  Fragments arranged by size: 

                 950     531      25
 
BtsI GCAGTG_nn'

Cuts at:      0      47    1506 
   Size:         47    1459
 
DraIII CAC_nnn'GTG

Cuts at:      0    1241    1506 
   Size:       1241     265
 
EaeI y'GGCC_r

Cuts at:      0     390    1035    1358    1506 
   Size:        390     645     323     148

  Fragments arranged by size: 

                 645     390     323     148
 
EarI CTCTTCn'nnn_

Cuts at:      0     451     745     928    1506 
   Size:        451     294     183     578

  Fragments arranged by size: 

                 578     451     294     183
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     521     655     726    1300    1395    1462    1506 
   Size:        217     304     134      71     574      95      67      44

  Fragments arranged by size: 

                 574     304     217     134      95      71      67      44
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1186    1506 
   Size:       1186     320
 
EcoO109I rG'GnC_Cy

Cuts at:      0     136     137    1230    1415    1506 
   Size:        136       1    1093     185      91

  Fragments arranged by size: 

                1093     185     136      91       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     316     477    1506 
   Size:        316     161    1029

  Fragments arranged by size: 

                1029     316     161
 
HaeII r_GCGC'y

Cuts at:      0     117     147    1222    1506 
   Size:        117      30    1075     284

  Fragments arranged by size: 

                1075     284     117      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       3      35     341     373    1506 
   Size:          3      32     306      32    1133

  Fragments arranged by size: 

                1133     306      32      32       3
 
KasI G'GCGC_C

Cuts at:      0     113     143    1506 
   Size:        113      30    1363

  Fragments arranged by size: 

                1363     113      30
 
KpnI G_GTAC'C

Cuts at:      0    1179    1196    1506 
   Size:       1179      17     310

  Fragments arranged by size: 

                1179     310      17
 
MscI TGG'CCA

Cuts at:      0     392    1037    1360    1506 
   Size:        392     645     323     146

  Fragments arranged by size: 

                 645     392     323     146
 
MspA1I CmG'CkG

Cuts at:      0     207     630    1506 
   Size:        207     423     876

  Fragments arranged by size: 

                 876     423     207
 
NarI GG'CG_CC

Cuts at:      0     114     144    1506 
   Size:        114      30    1362

  Fragments arranged by size: 

                1362     114      30
 
NcoI C'CATG_G

Cuts at:      0     700    1506 
   Size:        700     806
 
NdeI CA'TA_TG

Cuts at:      0     779    1506 
   Size:        779     727
 
NspI r_CATG'y

Cuts at:      0     680    1343    1506 
   Size:        680     663     163

  Fragments arranged by size: 

                 680     663     163
 
PasI CC'CwG_GG

Cuts at:      0    1142    1506 
   Size:       1142     364
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1241    1278    1506 
   Size:       1241      37     228

  Fragments arranged by size: 

                1241     228      37
 
PpuMI rG'GwC_Cy

Cuts at:      0    1230    1506 
   Size:       1230     276
 
PspOMI G'GGCC_C

Cuts at:      0     136    1506 
   Size:        136    1370
 
PstI C_TGCA'G

Cuts at:      0     264     816     910    1506 
   Size:        264     552      94     596

  Fragments arranged by size: 

                 596     552     264      94
 
PvuII CAG'CTG

Cuts at:      0     207     630    1506 
   Size:        207     423     876

  Fragments arranged by size: 

                 876     423     207
 
SanDI GG'GwC_CC

Cuts at:      0    1230    1506 
   Size:       1230     276
 
SapI GCTCTTCn'nnn_

Cuts at:      0     451    1506 
   Size:        451    1055
 
SfcI C'TryA_G

Cuts at:      0     260     812     906    1506 
   Size:        260     552      94     600

  Fragments arranged by size: 

                 600     552     260      94
 
SfoI GGC'GCC

Cuts at:      0     115     145    1506 
   Size:        115      30    1361

  Fragments arranged by size: 

                1361     115      30
 
SmaI CCC'GGG

Cuts at:      0    1066    1506 
   Size:       1066     440
 
SphI G_CATG'C

Cuts at:      0    1343    1506 
   Size:       1343     163
 
StyI C'CwwG_G

Cuts at:      0     156     700    1187    1506 
   Size:        156     544     487     319

  Fragments arranged by size: 

                 544     487     319     156
 
TatI w'GTAC_w

Cuts at:      0    1008    1506 
   Size:       1008     498
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     855     887    1248    1280    1506 
   Size:        855      32     361      32     226

  Fragments arranged by size: 

                 855     361     226      32      32
 
XmaI C'CCGG_G

Cuts at:      0    1064    1506 
   Size:       1064     442
 
XmnI GAAnn'nnTTC

Cuts at:      0     473    1506 
   Size:        473    1033
 

 Enzymes that do cut:

     AarI   Acc65I     AclI     AcuI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbeI     BglI     BlpI Bme1580I     BmrI     BpmI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspMI    BstXI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I     KasI     KpnI     MscI   MspA1I     NarI     NcoI     NdeI
     NspI     PasI    PflMI    PpuMI   PspOMI     PstI    PvuII    SanDI
     SapI     SfcI     SfoI     SmaI     SphI     StyI     TatI     TstI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI
    BsiEI    BsiWI    BsmBI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI
     MmeI     MslI     NaeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     ZraI
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