Restriction map for pF1KB0487
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BanI        BbeI        
BbvCI       BcgI        BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BsrI        BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HapII       HgaI        Hin1I       HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MflI        MluI        MroI        MspI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        StyI        SwaI        TspRI       
Tth111I     Van91I      XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI          472  GTAGAC               GT^[AC][GT]AC
AciI          256  CCGC                 CCGC(-3/-1)
AfaI         1520  GTAC                 GT^AC
AlwI         1285  GATCC                GGATC(4/5)
AlwNI         263  CAGTAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       460  AGCGCT               AGC^GCT
AseI          375  ATTAAT               AT^TAAT
AsnI          375  ATTAAT               AT^TAAT
AspHI         884  GAGCAC               G[AT]GC[AT]^C
BanII         980  GAGCCC               G[GA]GC[CT]^C
BbrPI         395  CACGTG               CAC^GTG
BpmI          101  CTCCAG               CTGGAG(16/14)
BsaAI         395  CACGTG               [CT]AC^GT[GA]
BsaBI         783  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1015  GGTCTC               GGTCTC(1/5)
BsiHKAI       884  GAGCAC               G[AT]GC[AT]^C
BsmFI        1052  GTCCC                GGGAC(10/14)
BspMI         904  GCAGGT               ACCTGC(4/8)
BssSI        1036  CTCGTG               CACGAG(-5/-1)
CfoI          461  GCGC                 GCG^C
Cfr13I       1041  GGGCC                G^G[ACGT]CC
DpnII        1285  GATC                 ^GATC
DraI          915  TTTAAA               TTT^AAA
EarI         1408  GAAGAG               CTCTTC(1/4)
Eco47III      460  AGCGCT               AGC^GCT
EcoNI         574  CCTGAATTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         353  GAATTC               G^AATTC
EcoRV         717  GATATC               GAT^ATC
HaeII         460  AGCGCT               [GA]GCGC^[CT]
HhaI          461  GCGC                 GCG^C
HinP1I        461  GCGC                 G^CGC
HindIII       661  AAGCTT               A^AGCTT
Ksp632I      1408  GAAGAG               CTCTTC(1/4)
MamI          783  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI         1285  GATC                 ^GATC
MfeI         1532  CAATTG               C^AATTG
MunI         1532  CAATTG               C^AATTG
NdeII        1285  GATC                 ^GATC
NspI          930  GCATGC               [GA]CATG^[CT]
PleI         1297  GACTC                GAGTC(4/5)
PmaCI         395  CACGTG               CAC^GTG
PmlI          395  CACGTG               CAC^GTG
PshBI         375  ATTAAT               AT^TAAT
PstI          710  CTGCAG               CTGCA^G
RsaI         1520  GTAC                 GT^AC
Sau3AI       1285  GATC                 ^GATC
Sau96I       1041  GGGCC                G^G[ACGT]CC
SfcI          710  CTGCAG               C^T[GA][CT]AG
SphI          930  GCATGC               GCATG^C
SspI          757  AATATT               AAT^ATT
Tsp45I       1295  GTGAC                ^GT[GC]AC
XbaI          991  TCTAGA               T^CTAGA
XcmI         1091  CCAAAACCGTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Asp700I       353  GAATTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1067  GAAAGATTTC          
BalI          839  TGGCCA               TGG^CCA
              923  TGGCCA              
BbsI         1195  GAAGAC               GAAGAC(2/6)
             1275  GAAGAC              
BmyI          884  GAGCAC               G[AGT]GC[ACT]^C
              980  GAGCCC              
BpuAI        1195  GAAGAC               GAAGAC(2/6)
             1275  GAAGAC              
BsiYI         574  CCTGAATTAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1006  CCTAAGTATGG         
BslI          574  CCTGAATTAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1006  CCTAAGTATGG         
BsmAI         438  GTCTC                GTCTC(1/5)
             1016  GTCTC               
BsmI         1079  GAATGC               GAATGC(1/-1)
              175  GCATTC              
Bsp1286I      884  GAGCAC               G[AGT]GC[ACT]^C
              980  GAGCCC              
BsrDI         843  CATTGC               GCAATG(2/0)
             1144  GCAATG              
BstF5I        369  GGATG                GGATG(2/0)
             1353  GGATG               
EaeI          839  TGGCCA               [CT]^GGCC[GA]
              923  TGGCCA              
FokI          369  GGATG                GGATG(9/13)
             1353  GGATG               
MaeII          19  ACGT                 A^CGT
              396  ACGT                
MluNI         839  TGGCCA               TGG^CCA
              923  TGGCCA              
MscI          839  TGGCCA               TGG^CCA
              923  TGGCCA              
MslI          774  CACCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1261  CACAAAAGTG          
PvuII         211  CAGCTG               CAG^CTG
              707  CAGCTG              
XmnI          353  GAATTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1067  GAAAGATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BciVI       
BfaI        BsaJI       BssKI       BstNI       Cac8I       
DdeI        EcoRII      Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MaeI        MaeIII      MboII       
MnlI        MseI        MspA1I      MvaI        MwoI        
NlaIII      ScrFI       SfaNI       TaqI        TfiI        
Tru9I       TseI        Tsp509I     TthHB8I     
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