(Circular) (Six-base) MAPSORT of: pF1KE1042.seq Check: 72 from: 1 to: 4616 pF1KE1042 4616 bp With 182 enzymes: SgfI * April 24, 2009 13:19 .. AccI GT'mk_AC Cuts at: 1654 1654 Size: 4616 Acc65I G'GTAC_C Cuts at: 1632 1632 Size: 4616 AclI AA'CG_TT Cuts at: 4456 4456 Size: 4616 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1431 2441 2873 3620 3888 1431 Size: 1010 432 747 268 2159 Fragments arranged by size: 2159 1010 747 432 268 AflIII A'CryG_T Cuts at: 1968 3072 4049 1968 Size: 1104 977 2535 Fragments arranged by size: 2535 1104 977 AgeI A'CCGG_T Cuts at: 260 3004 260 Size: 2744 1872 AhdI GACnn_n'nnGTC Cuts at: 512 512 Size: 4616 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 633 1527 633 Size: 894 3722 AlwNI CAG_nnn'CTG Cuts at: 825 1077 1921 3488 825 Size: 252 844 1567 1953 Fragments arranged by size: 1953 1567 844 252 ApaI G_GGCC'C Cuts at: 1357 1357 Size: 4616 ApaLI G'TGCA_C Cuts at: 3386 3386 Size: 4616 ApoI r'AATT_y Cuts at: 1551 1620 3969 4225 1551 Size: 69 2349 256 1942 Fragments arranged by size: 2349 1942 256 69 AseI AT'TA_AT Cuts at: 20 20 Size: 4616 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4616 AvaI C'yCGr_G Cuts at: 701 778 1636 701 Size: 77 858 3681 Fragments arranged by size: 3681 858 77 BamHI G'GATC_C Cuts at: 1641 1641 Size: 4616 BanI G'GyrC_C Cuts at: 609 1306 1632 2046 2294 2329 609 Size: 697 326 414 248 35 2896 Fragments arranged by size: 2896 697 414 326 248 35 BanII G_rGCy'C Cuts at: 856 911 1357 1610 1630 2660 856 Size: 55 446 253 20 1030 2812 Fragments arranged by size: 2812 1030 446 253 55 20 BbeI G_GCGC'C Cuts at: 2050 2298 2050 Size: 248 4368 BbsI GAAGACnn'nnnn_ Cuts at: 946 1158 946 Size: 212 4404 BbvCI CC'TCA_GC Cuts at: 292 292 Size: 4616 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 734 768 734 Size: 34 4582 BciVI GTATCCnnnnn_n' Cuts at: 2514 3275 4616 2514 Size: 761 1341 2514 Fragments arranged by size: 2514 1341 761 BclI T'GATC_A Cuts at: 2136 2136 Size: 4616 BglI GCCn_nnn'nGGC Cuts at: 485 485 Size: 4616 BglII A'GATC_T Cuts at: 1178 2131 4168 1178 Size: 953 2037 1626 Fragments arranged by size: 2037 1626 953 BlpI GC'TnA_GC Cuts at: 1728 1728 Size: 4616 Bme1580I G_kGCm'C Cuts at: 577 612 1357 2241 2334 3390 577 Size: 35 745 884 93 1056 1803 Fragments arranged by size: 1803 1056 884 745 93 35 BmgBI CAC'GTC Cuts at: 630 723 1458 630 Size: 93 735 3788 Fragments arranged by size: 3788 735 93 BmrI ACTGGGnnnn_n' Cuts at: 425 1828 1909 2244 4388 425 Size: 1403 81 335 2144 653 Fragments arranged by size: 2144 1403 653 335 81 BmtI G_CTAG'C Cuts at: 122 122 Size: 4616 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2983 2983 Size: 4616 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 163 583 883 952 1099 163 Size: 420 300 69 147 3680 Fragments arranged by size: 3680 420 300 147 69 Bpu10I CC'TnA_GC Cuts at: 292 589 292 Size: 297 4319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1081 1792 2671 3163 3461 1081 Size: 711 879 492 298 2236 Fragments arranged by size: 2236 879 711 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 878 4320 878 Size: 3442 1174 BsaAI yAC'GTr Cuts at: 1434 1560 1969 1434 Size: 126 409 4081 Fragments arranged by size: 4081 409 126 BsaBI GATnn'nnATC Cuts at: 6 2130 4167 6 Size: 2124 2037 455 Fragments arranged by size: 2124 2037 455 BsaHI Gr'CG_yC Cuts at: 1364 1442 2047 2295 2997 4493 1364 Size: 78 605 248 702 1496 1487 Fragments arranged by size: 1496 1487 702 605 248 78 BsaWI w'CCGG_w Cuts at: 260 1805 2326 3004 3278 3425 3836 260 Size: 1545 521 678 274 147 411 1040 Fragments arranged by size: 1545 1040 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 193 223 556 586 614 644 193 Size: 30 333 30 28 30 4165 Fragments arranged by size: 4165 333 30 30 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1098 1098 Size: 4616 BseYI C'CCAG_C Cuts at: 1242 2041 2799 3376 3872 1242 Size: 799 758 577 496 1986 Fragments arranged by size: 1986 799 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 838 1090 838 Size: 252 4364 BsiEI CG_ry'CG Cuts at: 104 422 2158 2204 3412 104 Size: 318 1736 46 1208 1308 Fragments arranged by size: 1736 1308 1208 318 46 BsiHKAI G_wGCw'C Cuts at: 211 569 812 836 856 911 1433 1630 2408 Size: 358 243 24 20 55 522 197 778 Cuts at: 2408 2598 3390 211 Size: 190 792 1437 Fragments arranged by size: 1437 792 778 522 358 243 197 190 55 24 20 BsmBI CGTCTCn'nnnn_ Cuts at: 775 1536 775 Size: 761 3855 Bsp1286I G_dGCh'C Cuts at: 211 569 577 612 812 836 856 911 1357 Size: 358 8 35 200 24 20 55 446 Cuts at: 1357 1433 1610 1630 2241 2334 2408 2598 2660 Size: 76 177 20 611 93 74 190 62 Cuts at: 2660 3390 211 Size: 730 1437 Fragments arranged by size: 1437 730 611 446 358 200 190 177 93 76 74 62 55 35 24 20 20 8 BspEI T'CCGG_A Cuts at: 3836 3836 Size: 4616 BspHI T'CATG_A Cuts at: 3792 3792 Size: 4616 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1250 1666 1880 2182 2563 1250 Size: 416 214 302 381 3303 Fragments arranged by size: 3303 416 381 302 214 BsrBI CCG'CTC Cuts at: 50 498 2908 2962 4446 4611 50 Size: 448 2410 54 1484 165 55 Fragments arranged by size: 2410 1484 448 165 55 54 BsrDI GCAATG_nn' Cuts at: 600 2528 600 Size: 1928 2688 BsrFI r'CCGG_y Cuts at: 260 417 596 1019 1028 2614 2795 3004 260 Size: 157 179 423 9 1586 181 209 1872 Fragments arranged by size: 1872 1586 423 209 181 179 157 9 BssSI C'ACGA_G Cuts at: 328 568 643 811 2887 3245 328 Size: 240 75 168 2076 358 1699 Fragments arranged by size: 2076 1699 358 240 168 75 BstAPI GCAn_nnn'nTGC Cuts at: 816 825 1993 816 Size: 9 1168 3439 Fragments arranged by size: 3439 1168 9 BstBI TT'CG_AA Cuts at: 2977 2977 Size: 4616 BstEII G'GTnAC_C Cuts at: 1395 1395 Size: 4616 BstYI r'GATC_y Cuts at: 1 695 1178 1641 2108 2131 2466 2712 3724 Size: 694 483 463 467 23 335 246 1012 Cuts at: 3724 3810 3822 4168 1 Size: 86 12 346 449 Fragments arranged by size: 1012 694 483 467 463 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4616 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1123 1485 2566 2747 2910 1123 Size: 362 1081 181 163 2829 Fragments arranged by size: 2829 1081 362 181 163 BtsI GCAGTG_nn' Cuts at: 256 826 1859 256 Size: 570 1033 3013 Fragments arranged by size: 3013 1033 570 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 714 749 714 Size: 35 4581 DraI TTT'AAA Cuts at: 1615 1615 Size: 4616 DraIII CAC_nnn'GTG Cuts at: 816 816 Size: 4616 DrdI GACnn_nn'nnGTC Cuts at: 2322 3180 2322 Size: 858 3758 EaeI y'GGCC_r Cuts at: 255 281 419 839 944 995 1218 2201 2375 Size: 26 138 420 105 51 223 983 174 Cuts at: 2375 2766 2793 4138 255 Size: 391 27 1345 733 Fragments arranged by size: 1345 983 733 420 391 223 174 138 105 51 27 26 EagI C'GGCC_G Cuts at: 419 2201 419 Size: 1782 2834 EarI CTCTTCn'nnn_ Cuts at: 1339 1525 2639 2849 1339 Size: 186 1114 210 3106 Fragments arranged by size: 3106 1114 210 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 201 526 2824 3134 3280 4426 201 Size: 325 2298 310 146 1146 391 Fragments arranged by size: 2298 1146 391 325 310 146 EcoICRI GAG'CTC Cuts at: 854 909 1628 854 Size: 55 719 3842 Fragments arranged by size: 3842 719 55 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 163 583 883 952 1099 1431 2441 2873 3620 Size: 420 300 69 147 332 1010 432 747 Cuts at: 3620 3888 163 Size: 268 891 Fragments arranged by size: 1010 891 747 432 420 332 300 268 147 69 EcoO109I rG'GnC_Cy Cuts at: 236 455 1101 1353 1755 236 Size: 219 646 252 402 3097 Fragments arranged by size: 3097 646 402 252 219 EcoRI G'AATT_C Cuts at: 1551 1620 1551 Size: 69 4547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 681 894 1416 2635 681 Size: 213 522 1219 2662 Fragments arranged by size: 2662 1219 522 213 FspI TGC'GCA Cuts at: 2397 2397 Size: 4616 HaeII r_GCGC'y Cuts at: 558 1203 2050 2298 3320 4299 558 Size: 645 847 248 1022 979 875 Fragments arranged by size: 1022 979 875 847 645 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 498 530 2458 2488 2490 2520 498 Size: 32 1928 30 2 30 2594 Fragments arranged by size: 2594 1928 32 30 30 2 HincII GTy'rAC Cuts at: 1488 1655 1488 Size: 167 4449 KasI G'GCGC_C Cuts at: 2046 2294 2046 Size: 248 4368 KpnI G_GTAC'C Cuts at: 1636 1636 Size: 4616 MluI A'CGCG_T Cuts at: 4049 4049 Size: 4616 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3287 3471 3287 Size: 184 4432 MscI TGG'CCA Cuts at: 257 283 841 997 2377 257 Size: 26 558 156 1380 2496 Fragments arranged by size: 2496 1380 558 156 26 MslI CAynn'nnrTG Cuts at: 2605 2732 2605 Size: 127 4489 MspA1I CmG'CkG Cuts at: 491 1077 1727 2041 2401 3414 3659 491 Size: 586 650 314 360 1013 245 1448 Fragments arranged by size: 1448 1013 650 586 360 314 245 NaeI GCC'GGC Cuts at: 419 598 1021 2797 419 Size: 179 423 1776 2238 Fragments arranged by size: 2238 1776 423 179 NarI GG'CG_CC Cuts at: 2047 2295 2047 Size: 248 4368 NcoI C'CATG_G Cuts at: 107 107 Size: 4616 NgoMIV G'CCGG_C Cuts at: 417 596 1019 2795 417 Size: 179 423 1776 2238 Fragments arranged by size: 2238 1776 423 179 NheI G'CTAG_C Cuts at: 118 118 Size: 4616 NspI r_CATG'y Cuts at: 1669 2169 3076 1669 Size: 500 907 3209 Fragments arranged by size: 3209 907 500 PciI A'CATG_T Cuts at: 3072 3072 Size: 4616 PmeI GTTT'AAAC Cuts at: 1615 1615 Size: 4616 PmlI CAC'GTG Cuts at: 1434 1434 Size: 4616 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 3755 Size: 32 4584 PpuMI rG'GwC_Cy Cuts at: 455 1101 455 Size: 646 3970 PspOMI G'GGCC_C Cuts at: 1353 1353 Size: 4616 PstI C_TGCA'G Cuts at: 117 220 917 1018 1663 2348 117 Size: 103 697 101 645 685 2385 Fragments arranged by size: 2385 697 685 645 103 101 PvuI CG_AT'CG Cuts at: 104 104 Size: 4616 PvuII CAG'CTG Cuts at: 1077 2041 2401 1077 Size: 964 360 3292 Fragments arranged by size: 3292 964 360 RsrII CG'GwC_CG Cuts at: 2811 2811 Size: 4616 SacI G_AGCT'C Cuts at: 856 911 1630 856 Size: 55 719 3842 Fragments arranged by size: 3842 719 55 SalI G'TCGA_C Cuts at: 1653 1653 Size: 4616 SanDI GG'GwC_CC Cuts at: 455 455 Size: 4616 SapI GCTCTTCn'nnn_ Cuts at: 2639 2849 2639 Size: 210 4406 SbfI CC_TGCA'GG Cuts at: 1663 1663 Size: 4616 ScaI AGT'ACT Cuts at: 4133 4133 Size: 4616 SfcI C'TryA_G Cuts at: 33 113 151 216 913 1014 1659 2344 3337 Size: 80 38 65 697 101 645 685 993 Cuts at: 3337 3528 33 Size: 191 1121 Fragments arranged by size: 1121 993 697 685 645 191 101 80 65 38 SfoI GGC'GCC Cuts at: 2048 2296 2048 Size: 248 4368 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4616 SmaI CCC'GGG Cuts at: 703 1638 703 Size: 935 3681 SmlI C'TyrA_G Cuts at: 778 1096 1771 2686 3178 3440 778 Size: 318 675 915 492 262 1954 Fragments arranged by size: 1954 915 675 492 318 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4616 SphI G_CATG'C Cuts at: 1669 2169 1669 Size: 500 4116 StuI AGG'CCT Cuts at: 530 1012 530 Size: 482 4134 StyI C'CwwG_G Cuts at: 107 540 1750 2104 107 Size: 433 1210 354 2619 Fragments arranged by size: 2619 1210 433 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 523 2199 2332 2791 3000 523 Size: 1676 133 459 209 2139 Fragments arranged by size: 2139 1676 459 209 133 TatI w'GTAC_w Cuts at: 4131 4131 Size: 4616 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 556 588 614 646 4133 4165 556 Size: 32 26 32 3487 32 1007 Fragments arranged by size: 3487 1007 32 32 32 26 Tth111I GACn'n_nGTC Cuts at: 721 2413 721 Size: 1692 2924 XbaI T'CTAG_A Cuts at: 1647 1647 Size: 4616 XcmI CCAnnnn_n'nnnnTGG Cuts at: 174 250 2111 174 Size: 76 1861 2679 Fragments arranged by size: 2679 1861 76 XhoI C'TCGA_G Cuts at: 778 778 Size: 4616 XmaI C'CCGG_G Cuts at: 701 1636 701 Size: 935 3681 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AloI AscI AvrII BaeI BfrBI BsiWI BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI PacI PasI PflMI PfoI PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI