Restriction map for pF1KB6346
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AvaI        AviII       AvrII       BalI        
BanI        BbeI        BbsI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaI        BsgI        
BsiWI       BsmI        BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MscI        
MseI        MunI        NarI        NcoI        NdeI        
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        TfiI        Tru9I       TspRI       
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AatII         799  GACGTC               GACGT^C
AccII         558  CGCG                 CG^CG
AccIII        109  TCCGGA               T^CCGGA
AcsI          595  AAATTT               [GA]^AATT[CT]
AfaI          732  GTAC                 GT^AC
AlwNI          25  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          655  GGGCCC               GGGCC^C
ApoI          595  AAATTT               [GA]^AATT[CT]
Asp700I       381  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          796  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         178  GGATCC               G^GATCC
BbrPI         376  CACGTG               CAC^GTG
BbvCI         141  CCTCAGC              CCTCAGC(-5/-2)
BcgI          756  CGAGGACTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          313  CTAG                 C^TAG
BsaAI         376  CACGTG               [CT]AC^GT[GA]
BsaWI         109  TCCGGA               [AT]^CCGG[AT]
BseAI         109  TCCGGA               T^CCGGA
BsiEI         783  CGGCCG               CG[GA][CT]^CG
BspEI         109  TCCGGA               T^CCGGA
BsrFI         545  GCCGGC               [GA]^CCGG[CT]
BssSI         481  CACGAG               CACGAG(-5/-1)
BstF5I        632  GGATG                GGATG(2/0)
BstUI         558  CGCG                 CG^CG
BstXI         432  CCAGGGCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         178  GGATCC               [GA]^GATC[CT]
Cfr10I        545  GCCGGC               [GA]^CCGG[CT]
DraIII        562  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          738  CCACGG               C^C[GA][CT]GG
EagI          783  CGGCCG               C^GGCCG
EclXI         783  CGGCCG               C^GGCCG
Eco52I        783  CGGCCG               C^GGCCG
EcoT14I         5  CCAAGG               C^C[AT][AT]GG
FauI          210  GCGGG                CCCGC(4/6)
FokI          632  GGATG                GGATG(9/13)
HaeII         426  GGCGCT               [GA]GCGC^[CT]
HincII        466  GTCAAC               GT[CT]^[GA]AC
HindII        466  GTCAAC               GT[CT]^[GA]AC
HinfI         316  GAGTC                G^A[ACGT]TC
HphI          773  TCACC                GGTGA(8/7)
MaeI          313  CTAG                 C^TAG
MflI          178  GGATCC               [GA]^GATC[CT]
MroI          109  TCCGGA               T^CCGGA
MslI          627  CACCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          558  CGCG                 CG^CG
NaeI          545  GCCGGC               GCC^GGC
NgoMIV        545  GCCGGC               G^CCGGC
PflFI         796  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          316  GAGTC                GAGTC(4/5)
PmaCI         376  CACGTG               CAC^GTG
PmlI          376  CACGTG               CAC^GTG
RsaI          732  GTAC                 GT^AC
SexAI         563  ACCTGGT              A^CC[AT]GGT
SfaNI          27  GCATC                GCATC(5/9)
SmlI          270  CTTGAG               C^T[CT][GA]AG
StuI          518  AGGCCT               AGG^CCT
StyI            5  CCAAGG               C^C[AT][AT]GG
Tsp509I       596  AATT                 ^AATT
Tth111I       796  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         178  GGATCC               [GA]^GATC[CT]
XmnI          381  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI          529  GACGCC               G[GA]^CG[CT]C
              799  GACGTC              
AlwI          179  GATCC                GGATC(4/5)
              178  GGATC               
BanII         655  GGGCCC               G[GA]GC[CT]^C
              742  GGGCTC              
BcnI          462  CCCGG                CC^[GC]GG
              512  CCGGG               
BsaHI         529  GACGCC               G[GA]^CG[CT]C
              799  GACGTC              
BsmFI         676  GGGAC                GGGAC(10/14)
              318  GTCCC               
BspMI         398  ACCTGC               ACCTGC(4/8)
              454  ACCTGC              
BsrDI         287  GCAATG               GCAATG(2/0)
              437  GCAATG              
EaeI          735  CGGCCA               [CT]^GGCC[GA]
              783  CGGCCG              
Hin1I         529  GACGCC               G[GA]^CG[CT]C
              799  GACGTC              
MaeIII         47  GTGAC                ^GT[ACGT]AC
              574  GTCAC               
MboII         296  GAAGA                GAAGA(8/7)
              352  GAAGA               
NciI          462  CCCGG                CC^[GC]GG
              512  CCGGG               
PpuMI         341  AGGACCT              [GA]G^G[AT]CC[CT]
              451  AGGACCT             
PstI           94  CTGCAG               CTGCA^G
              196  CTGCAG              
SfcI           94  CTGCAG               C^T[GA][CT]AG
              196  CTGCAG              
Tsp45I         47  GTGAC                ^GT[GC]AC
              574  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AspHI       AvaII       BbvI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       Bsp1286I    
BsrI        BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       ItaI        MaeII       
MboI        MnlI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIV       PvuII       Sau3AI      
Sau96I      ScrFI       TseI        
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