Restriction map for pF1KB6346
 (Linear) (Six-base) MAPSORT of: KIBB6346.seq  Check: 1026  from: 1  to: 873



KIBB6346.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AatII G_ACGT'C

Cuts at:      0     803     873 
   Size:        803      70
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     873 
   Size:          7     866
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30     873 
   Size:         30     843
 
ApaI G_GGCC'C

Cuts at:      0     659     873 
   Size:        659     214
 
ApoI r'AATT_y

Cuts at:      0     595     873 
   Size:        595     278
 
BamHI G'GATC_C

Cuts at:      0     178     873 
   Size:        178     695
 
BanII G_rGCy'C

Cuts at:      0     659     746     873 
   Size:        659      87     127

  Fragments arranged by size: 

                 659     127      87
 
BbvCI CC'TCA_GC

Cuts at:      0     142     873 
   Size:        142     731
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     745     779     873 
   Size:        745      34      94

  Fragments arranged by size: 

                 745      94      34
 
Bme1580I G_kGCm'C

Cuts at:      0     659     768     873 
   Size:        659     109     105

  Fragments arranged by size: 

                 659     109     105
 
BmgBI CAC'GTC

Cuts at:      0     578     873 
   Size:        578     295
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     480     873 
   Size:        480     393
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     346     415     571     726     873 
   Size:        345       1      69     156     155     147

  Fragments arranged by size: 

                 345     156     155     147      69       1
 
Bpu10I CC'TnA_GC

Cuts at:      0     142     409     873 
   Size:        142     267     464

  Fragments arranged by size: 

                 464     267     142
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     873 
   Size:        291     582
 
BsaAI yAC'GTr

Cuts at:      0     378     873 
   Size:        378     495
 
BsaHI Gr'CG_yC

Cuts at:      0     530     800     873 
   Size:        530     270      73

  Fragments arranged by size: 

                 530     270      73
 
BsaWI w'CCGG_w

Cuts at:      0     109     873 
   Size:        109     764
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     104     134     275     305     873 
   Size:        104      30     141      30     568

  Fragments arranged by size: 

                 568     141     104      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55      68     112     217     601     764     873 
   Size:         55      13      44     105     384     163     109

  Fragments arranged by size: 

                 384     163     109     105      55      44      13
 
BseYI C'CCAG_C

Cuts at:      0      83     191     320     821     873 
   Size:         83     108     129     501      52

  Fragments arranged by size: 

                 501     129     108      83      52
 
BsiEI CG_ry'CG

Cuts at:      0     786     873 
   Size:        786      87
 
BsiHKAI G_wGCw'C

Cuts at:      0     250     277     482     873 
   Size:        250      27     205     391

  Fragments arranged by size: 

                 391     250     205      27
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     106     217     682     873 
   Size:        106     111     465     191

  Fragments arranged by size: 

                 465     191     111     106
 
Bsp1286I G_dGCh'C

Cuts at:      0     250     277     482     659     746     768     873 
   Size:        250      27     205     177      87      22     105

  Fragments arranged by size: 

                 250     205     177     105      87      27      22
 
BspEI T'CCGG_A

Cuts at:      0     109     873 
   Size:        109     764
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     407     463     873 
   Size:        407      56     410

  Fragments arranged by size: 

                 410     407      56
 
BsrDI GCAATG_nn'

Cuts at:      0     294     444     873 
   Size:        294     150     429

  Fragments arranged by size: 

                 429     294     150
 
BsrFI r'CCGG_y

Cuts at:      0     545     873 
   Size:        545     328
 
BssSI C'ACGA_G

Cuts at:      0     481     873 
   Size:        481     392
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     439     873 
   Size:        439     434
 
BstYI r'GATC_y

Cuts at:      0     178     873 
   Size:        178     695
 
BtgI C'CryG_G

Cuts at:      0     738     873 
   Size:        738     135
 
DraIII CAC_nnn'GTG

Cuts at:      0     567     873 
   Size:        567     306
 
EaeI y'GGCC_r

Cuts at:      0     735     783     873 
   Size:        735      48      90

  Fragments arranged by size: 

                 735      90      48
 
EagI C'GGCC_G

Cuts at:      0     783     873 
   Size:        783      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     345     346     415     571     726     873 
   Size:          7     338       1      69     156     155     147

  Fragments arranged by size: 

                 338     156     155     147      69       7       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0     213     342     452     616     656     812     873 
   Size:        213     129     110     164      40     156      61

  Fragments arranged by size: 

                 213     164     156     129     110      61      40
 
HaeII r_GCGC'y

Cuts at:      0     430     873 
   Size:        430     443
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     339     371     873 
   Size:        104      32     203      32     502

  Fragments arranged by size: 

                 502     203     104      32      32
 
HincII GTy'rAC

Cuts at:      0     468     873 
   Size:        468     405
 
MslI CAynn'nnrTG

Cuts at:      0     631     873 
   Size:        631     242
 
MspA1I CmG'CkG

Cuts at:      0     195     324     491     788     821     873 
   Size:        195     129     167     297      33      52

  Fragments arranged by size: 

                 297     195     167     129      52      33
 
NaeI GCC'GGC

Cuts at:      0     547     873 
   Size:        547     326
 
NgoMIV G'CCGG_C

Cuts at:      0     545     873 
   Size:        545     328
 
PasI CC'CwG_GG

Cuts at:      0     135     279     873 
   Size:        135     144     594

  Fragments arranged by size: 

                 594     144     135
 
PfoI T'CCnGG_A

Cuts at:      0     511     873 
   Size:        511     362
 
PmlI CAC'GTG

Cuts at:      0     378     873 
   Size:        378     495
 
PpuMI rG'GwC_Cy

Cuts at:      0     342     452     873 
   Size:        342     110     421

  Fragments arranged by size: 

                 421     342     110
 
PspOMI G'GGCC_C

Cuts at:      0     655     873 
   Size:        655     218
 
PstI C_TGCA'G

Cuts at:      0      98     200     873 
   Size:         98     102     673

  Fragments arranged by size: 

                 673     102      98
 
PvuII CAG'CTG

Cuts at:      0     195     324     491     873 
   Size:        195     129     167     382

  Fragments arranged by size: 

                 382     195     167     129
 
SexAI A'CCwGG_T

Cuts at:      0     563     873 
   Size:        563     310
 
SfcI C'TryA_G

Cuts at:      0      94     196     873 
   Size:         94     102     677

  Fragments arranged by size: 

                 677     102      94
 
SmlI C'TyrA_G

Cuts at:      0     270     873 
   Size:        270     603
 
StuI AGG'CCT

Cuts at:      0     520     873 
   Size:        520     353
 
StyI C'CwwG_G

Cuts at:      0       5     873 
   Size:          5     868
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     292     462     657     873 
   Size:        292     170     195     216

  Fragments arranged by size: 

                 292     216     195     170
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     275     307     873 
   Size:        275      32     566

  Fragments arranged by size: 

                 566     275      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     799     873 
   Size:        799      74
 
XmnI GAAnn'nnTTC

Cuts at:      0     385     873 
   Size:        385     488
 
ZraI GAC'GTC

Cuts at:      0     801     873 
   Size:        801      72
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI     ApaI     ApoI    BamHI    BanII    BbvCI
     BcgI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BssSI    BstXI    BstYI
     BtgI   DraIII     EaeI     EagI  Eco57MI EcoO109I    HaeII    Hin4I
   HincII     MslI   MspA1I     NaeI   NgoMIV     PasI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII    SexAI     SfcI     SmlI     StuI
     StyI    TaqII     TstI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmtI     BplI     BsaI    BsaBI
     BsgI    BsiWI     BsmI    BspHI    BsrBI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     XbaI     XcmI     XhoI     XmaI
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