Restriction map for pF1KB7682
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# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccII       AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        AspEI       
AvaI        AviII       AvrII       BalI        BamHI       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        HpaI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MscI        MunI        MvnI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tsp45I      Tsp509I     XbaI        
XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I        339  GGTACC               G^GTACC
AccIII        738  TCCGGA               T^CCGGA
AcyI           12  GGCGCC               G[GA]^CG[CT]C
Alw44I       1071  GTGCAC               G^TGCAC
AlwNI         505  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1071  GTGCAC               G^TGCAC
Asp700I      1053  GAAATGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       339  GGTACC               G^GTACC
AspHI        1071  GTGCAC               G[AT]GC[AT]^C
AspI          686  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI           12  GGCGCC               GGCGC^C
BlpI          646  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      646  GCTCAGC              GC^T[ACGT]AGC
BsaHI          12  GGCGCC               G[GA]^CG[CT]C
BsaI          684  GAGACC               GGTCTC(1/5)
BseAI         738  TCCGGA               T^CCGGA
BseRI         674  CTCCTC               GAGGAG(10/8)
BsgI          316  GTGCAG               GTGCAG(16/14)
BsiHKAI      1071  GTGCAC               G[AT]GC[AT]^C
BsmAI         684  GAGAC                GTCTC(1/5)
BspEI         738  TCCGGA               T^CCGGA
BsrFI         100  GCCGGT               [GA]^CCGG[CT]
BstAPI        540  GCAGATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1160  AGATCC               [GA]^GATC[CT]
CelII         646  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        100  GCCGGT               [GA]^CCGG[CT]
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EarI          114  GAAGAG               CTCTTC(1/4)
EcoNI         964  CCTCCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I          12  GGCGCC               G[GA]^CG[CT]C
HincII        389  GTCGAC               GT[CT]^[GA]AC
HindII        389  GTCGAC               GT[CT]^[GA]AC
HindIII      1115  AAGCTT               A^AGCTT
KasI           12  GGCGCC               G^GCGCC
KpnI          339  GGTACC               GGTAC^C
Ksp632I       114  GAAGAG               CTCTTC(1/4)
MflI         1160  AGATCC               [GA]^GATC[CT]
MroI          738  TCCGGA               T^CCGGA
MseI          851  TTAA                 T^TAA
MslI         1074  CACAGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1195  CAGCTG               C[AC]G^C[GT]G
NarI           12  GGCGCC               GG^CGCC
NspI          437  ACATGC               [GA]CATG^[CT]
PflFI         686  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          117  GAGTC                GAGTC(4/5)
PpuMI         450  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII        1195  CAGCTG               CAG^CTG
SalI          389  GTCGAC               G^TCGAC
SfcI          351  CTACAG               C^T[GA][CT]AG
SfoI           12  GGCGCC               GGC^GCC
SmlI         1147  CTCAAG               C^T[CT][GA]AG
StuI         1040  AGGCCT               AGG^CCT
TaqI          390  TCGA                 T^CGA
TfiI          789  GAATC                G^A[AT]TC
Tru9I         851  TTAA                 T^TAA
Tth111I       686  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       390  TCGA                 T^CGA
XcmI          605  CCAGAGCAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1160  AGATCC               [GA]^GATC[CT]
XmnI         1053  GAAATGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI          389  GTCGAC               GT^[AC][GT]AC
             1171  GTCTAC              
AfaI          340  GTAC                 GT^AC
              981  GTAC                
AlwI         1161  GATCC                GGATC(4/5)
              207  GGATC               
BglI            4  GCCGCCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1123  GCCGTCTGGGC         
BsaWI          24  ACCGGA               [AT]^CCGG[AT]
              738  TCCGGA              
BspMI         795  ACCTGC               ACCTGC(4/8)
              940  GCAGGT              
DsaI          200  CCATGG               C^C[GA][CT]GG
              590  CCATGG              
HaeII          12  GGCGCC               [GA]GCGC^[CT]
             1090  AGCGCC              
HinfI         117  GAGTC                G^A[ACGT]TC
              789  GAATC               
MboII         114  GAAGA                GAAGA(8/7)
              235  GAAGA               
NcoI          200  CCATGG               C^CATGG
              590  CCATGG              
PflMI         277  CCATTCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              504  CCAGCCTCTGG         
RsaI          340  GTAC                 GT^AC
              981  GTAC                
SexAI         212  ACCTGGT              A^CC[AT]GGT
              687  ACCTGGT             
Van91I        277  CCATTCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              504  CCAGCCTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        ApaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MboI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TseI        TspRI       
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