Restriction map for pF1KB7682
 (Linear) (Six-base) MAPSORT of: KIBB7682.seq  Check: 1784  from: 1  to: 1218



KIBB7682.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     804     931    1218 
   Size:        804     127     287

  Fragments arranged by size: 

                 804     287     127
 
AccI GT'mk_AC

Cuts at:      0     390    1172    1218 
   Size:        390     782      46

  Fragments arranged by size: 

                 782     390      46
 
Acc65I G'GTAC_C

Cuts at:      0     339    1218 
   Size:        339     879
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1133    1218 
   Size:       1133      85
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     310    1218 
   Size:        310     908
 
AlwNI CAG_nnn'CTG

Cuts at:      0     510    1218 
   Size:        510     708
 
ApaI G_GGCC'C

Cuts at:      0     157     376     427     556     622     959    1218 
   Size:        157     219      51     129      66     337     259

  Fragments arranged by size: 

                 337     259     219     157     129      66      51
 
ApaLI G'TGCA_C

Cuts at:      0    1071    1218 
   Size:       1071     147
 
BanI G'GyrC_C

Cuts at:      0      12     339     924     991    1218 
   Size:         12     327     585      67     227

  Fragments arranged by size: 

                 585     327     227      67      12
 
BanII G_rGCy'C

Cuts at:      0     157     252     376     427     556     622     660     701 
   Size:        157      95     124      51     129      66      38      41

Cuts at:    701     824     952     959    1218 
   Size:        123     128       7     259

  Fragments arranged by size: 

                 259     157     129     128     124     123      95      66
                  51      41      38       7
 
BbeI G_GCGC'C

Cuts at:      0      16    1218 
   Size:         16    1202
 
BglI GCCn_nnn'nGGC

Cuts at:      0      10    1129    1218 
   Size:         10    1119      89

  Fragments arranged by size: 

                1119      89      10
 
BlpI GC'TnA_GC

Cuts at:      0     647    1218 
   Size:        647     571
 
Bme1580I G_kGCm'C

Cuts at:      0     157     299     376     427     556     622     904     959 
   Size:        157     142      77      51     129      66     282      55

Cuts at:    959    1075    1218 
   Size:        116     143

  Fragments arranged by size: 

                 282     157     143     142     129     116      77      66
                  55      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     527     550     860    1218 
   Size:        527      23     310     358

  Fragments arranged by size: 

                 527     358     310      23
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     843    1218 
   Size:        843     375
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     380     664     890    1218 
   Size:         55     325     284     226     328

  Fragments arranged by size: 

                 328     325     284     226      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1132    1218 
   Size:       1132      86
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     678    1218 
   Size:        678     540
 
BsaHI Gr'CG_yC

Cuts at:      0      13    1218 
   Size:         13    1205
 
BsaWI w'CCGG_w

Cuts at:      0      24     738    1218 
   Size:         24     714     480

  Fragments arranged by size: 

                 714     480      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     817     847    1150    1180    1218 
   Size:        817      30     303      30      38

  Fragments arranged by size: 

                 817     303      38      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     665    1218 
   Size:        665     553
 
BseYI C'CCAG_C

Cuts at:      0      73     377     487     615     703     959    1218 
   Size:         73     304     110     128      88     256     259

  Fragments arranged by size: 

                 304     259     256     128     110      88      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337    1218 
   Size:        337     881
 
BsiHKAI G_wGCw'C

Cuts at:      0    1075    1218 
   Size:       1075     143
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     252     299     376     427     556     622     660 
   Size:        157      95      47      77      51     129      66      38

Cuts at:    660     701     824     904     952     959    1075    1218 
   Size:         41     123      80      48       7     116     143

  Fragments arranged by size: 

                 157     143     129     123     116      95      80      77
                  66      51      48      47      41      38       7
 
BspEI T'CCGG_A

Cuts at:      0     738    1218 
   Size:        738     480
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     804     931    1218 
   Size:        804     127     287

  Fragments arranged by size: 

                 804     287     127
 
BsrFI r'CCGG_y

Cuts at:      0     100    1218 
   Size:        100    1118
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     546    1218 
   Size:        546     672
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     420     454     688     921    1218 
   Size:        420      34     234     233     297

  Fragments arranged by size: 

                 420     297     234     233      34
 
BstYI r'GATC_y

Cuts at:      0    1160    1218 
   Size:       1160      58
 
BtgI C'CryG_G

Cuts at:      0     200     590    1218 
   Size:        200     390     628

  Fragments arranged by size: 

                 628     390     200
 
BtsI GCAGTG_nn'

Cuts at:      0     891    1218 
   Size:        891     327
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     924     959    1218 
   Size:        924      35     259

  Fragments arranged by size: 

                 924     259      35
 
EaeI y'GGCC_r

Cuts at:      0       2    1218 
   Size:          2    1216
 
EarI CTCTTCn'nnn_

Cuts at:      0     109    1218 
   Size:        109    1109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     380     664     890    1133    1218 
   Size:         55     325     284     226     243      85

  Fragments arranged by size: 

                 325     284     243     226      85      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     968    1218 
   Size:        968     250
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153     154     372     373     424     451     956    1218 
   Size:        153       1     218       1      51      27     505     262

  Fragments arranged by size: 

                 505     262     218     153      51      27       1       1
            
 
HaeII r_GCGC'y

Cuts at:      0      16    1094    1218 
   Size:         16    1078     124

  Fragments arranged by size: 

                1078     124      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     675     707    1150    1182    1218 
   Size:        675      32     443      32      36

  Fragments arranged by size: 

                 675     443      36      32      32
 
HincII GTy'rAC

Cuts at:      0     391    1218 
   Size:        391     827
 
HindIII A'AGCT_T

Cuts at:      0    1115    1218 
   Size:       1115     103
 
KasI G'GCGC_C

Cuts at:      0      12    1218 
   Size:         12    1206
 
KpnI G_GTAC'C

Cuts at:      0     343    1218 
   Size:        343     875
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     979    1218 
   Size:        248     731     239

  Fragments arranged by size: 

                 731     248     239
 
MslI CAynn'nnrTG

Cuts at:      0    1078    1218 
   Size:       1078     140
 
MspA1I CmG'CkG

Cuts at:      0    1197    1218 
   Size:       1197      21
 
NarI GG'CG_CC

Cuts at:      0      13    1218 
   Size:         13    1205
 
NcoI C'CATG_G

Cuts at:      0     200     590    1218 
   Size:        200     390     628

  Fragments arranged by size: 

                 628     390     200
 
NspI r_CATG'y

Cuts at:      0     441    1218 
   Size:        441     777
 
PasI CC'CwG_GG

Cuts at:      0     447    1218 
   Size:        447     771
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     283     510    1218 
   Size:        283     227     708

  Fragments arranged by size: 

                 708     283     227
 
PpuMI rG'GwC_Cy

Cuts at:      0     451    1218 
   Size:        451     767
 
PspOMI G'GGCC_C

Cuts at:      0     153     372     423     552     618     955    1218 
   Size:        153     219      51     129      66     337     263

  Fragments arranged by size: 

                 337     263     219     153     129      66      51
 
PvuII CAG'CTG

Cuts at:      0    1197    1218 
   Size:       1197      21
 
SalI G'TCGA_C

Cuts at:      0     389    1218 
   Size:        389     829
 
SexAI A'CCwGG_T

Cuts at:      0     212     687    1218 
   Size:        212     475     531

  Fragments arranged by size: 

                 531     475     212
 
SfcI C'TryA_G

Cuts at:      0     351    1218 
   Size:        351     867
 
SfoI GGC'GCC

Cuts at:      0      14    1218 
   Size:         14    1204
 
SmlI C'TyrA_G

Cuts at:      0    1147    1218 
   Size:       1147      71
 
StuI AGG'CCT

Cuts at:      0    1042    1218 
   Size:       1042     176
 
StyI C'CwwG_G

Cuts at:      0     200     282     590    1154    1218 
   Size:        200      82     308     564      64

  Fragments arranged by size: 

                 564     308     200      82      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     600    1218 
   Size:        600     618
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     389     421     817     849    1218 
   Size:        389      32     396      32     369

  Fragments arranged by size: 

                 396     389     369      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     689    1218 
   Size:        689     529
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     612    1218 
   Size:        612     606
 
XmnI GAAnn'nnTTC

Cuts at:      0    1057    1218 
   Size:       1057     161
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AlfI    AlwNI     ApaI    ApaLI
     BanI    BanII     BbeI     BglI     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrFI   BstAPI    BstXI
    BstYI     BtgI     BtsI    CspCI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MmeI
     MslI   MspA1I     NarI     NcoI     NspI     PasI    PflMI    PpuMI
   PspOMI    PvuII     SalI    SexAI     SfcI     SfoI     SmlI     StuI
     StyI    TaqII     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AloI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmtI   Bpu10I    BsaAI    BsaBI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI     HpaI     MfeI     MluI     MscI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     XbaI     XhoI     XmaI     ZraI
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