Restriction map for pF1KB9983
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccI        AccII       AclI        AflII       
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        AspEI       AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BbvCI       BcgI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     FseI        FspI        
HgaI        HindIII     HpaI        KspI        MfeI        
MluI        MluNI       MscI        MunI        MvnI        
NaeI        NcoI        NdeI        NgoMIV      NotI        
NruI        NspV        PacI        PflMI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

Acc65I        844  GGTACC               G^GTACC
AccIII       1086  TCCGGA               T^CCGGA
AcyI         1477  GGCGCC               G[GA]^CG[CT]C
AflIII       1471  ACATGT               A^C[GA][CT]GT
AgeI          784  ACCGGT               A^CCGGT
Alw44I       1510  GTGCAC               G^TGCAC
AlwI          216  GGATC                GGATC(4/5)
AocI         1515  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1510  GTGCAC               G^TGCAC
Asp718I       844  GGTACC               G^GTACC
AspI         1166  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          464  CTCGAG               C^[CT]CG[GA]G
BbeI         1477  GGCGCC               GGCGC^C
BbsI         1005  GTCTTC               GAAGAC(2/6)
BciVI        1119  GTATCC               GTATCC(6/5)
BclI          945  TGATCA               T^GATCA
BpuAI        1005  GTCTTC               GAAGAC(2/6)
BsaHI        1477  GGCGCC               G[GA]^CG[CT]C
BseAI        1086  TCCGGA               T^CCGGA
BsgI          498  GTGCAG               GTGCAG(16/14)
BsoBI         464  CTCGAG               C^[CT]CG[GA]G
BspDI        1100  ATCGAT               AT^CGAT
BspEI        1086  TCCGGA               T^CCGGA
BspHI         368  TCATGA               T^CATGA
BspLU11I     1471  ACATGT               A^CATGT
BsrDI        1137  CATTGC               GCAATG(2/0)
BsrFI         784  ACCGGT               [GA]^CCGG[CT]
BstAPI       1245  GCAGCACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         216  GGATCT               [GA]^GATC[CT]
Bsu36I       1515  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        784  ACCGGT               [GA]^CCGG[CT]
ClaI         1100  ATCGAT               AT^CGAT
DraII         983  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eco81I       1515  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      983  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         624  GAATTC               G^AATTC
EcoRV          94  GATATC               GAT^ATC
EcoT14I       685  CCTTGG               C^C[AT][AT]GG
EcoT22I      1330  ATGCAT               ATGCA^T
FauI         1211  GCGGG                CCCGC(4/6)
FbaI          945  TGATCA               T^GATCA
HaeII        1477  GGCGCC               [GA]GCGC^[CT]
Hin1I        1477  GGCGCC               G[GA]^CG[CT]C
HincII        664  GTTGAC               GT[CT]^[GA]AC
HindII        664  GTTGAC               GT[CT]^[GA]AC
KasI         1477  GGCGCC               G^GCGCC
KpnI          844  GGTACC               GGTAC^C
MaeII        1066  ACGT                 A^CGT
MflI          216  GGATCT               [GA]^GATC[CT]
MroI         1086  TCCGGA               T^CCGGA
MspA1I       1290  CCGCTG               C[AC]G^C[GT]G
NarI         1477  GGCGCC               GG^CGCC
NheI         1545  GCTAGC               G^CTAGC
NsiI         1330  ATGCAT               ATGCA^T
PaeR7I        464  CTCGAG               C^TCGAG
PflFI        1166  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         784  ACCGGT               A^CCGGT
PpuMI         983  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          368  TCATGA               T^CATGA
SfcI         1013  CTATAG               C^T[GA][CT]AG
SfoI         1477  GGCGCC               GGC^GCC
SmlI          464  CTCGAG               C^T[CT][GA]AG
SspI          298  AATATT               AAT^ATT
StyI          685  CCTTGG               C^C[AT][AT]GG
Tth111I      1166  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          420  TCTAGA               T^CTAGA
XcmI           42  CCAGGAAATGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          464  CTCGAG               C^TCGAG
XhoII         216  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI          624  GAATTC               [GA]^AATT[CT]
             1500  AAATTT              
AlwNI         233  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              351  CAGCACCTG           
ApoI          624  GAATTC               [GA]^AATT[CT]
             1500  AAATTT              
Asp700I      1108  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1299  GAAAAAGTTC          
AspHI         819  GAGCAC               G[AT]GC[AT]^C
             1510  GTGCAC              
BsaBI         217  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1093  GATGATGATC          
BsaI          781  GAGACC               GGTCTC(1/5)
             1609  GAGACC              
BsiHKAI       819  GAGCAC               G[AT]GC[AT]^C
             1510  GTGCAC              
BsmFI         909  GTCCC                GGGAC(10/14)
             1181  GTCCC               
BspMI         355  ACCTGC               ACCTGC(4/8)
             1207  ACCTGC              
BsrI          238  ACTGG                ACTGG(1/-1)
              661  CCAGT               
CfoI          549  GCGC                 GCG^C
             1478  GCGC                
HaeIII        184  GGCC                 GG^CC
              772  GGCC                
HhaI          549  GCGC                 GCG^C
             1478  GCGC                
HinP1I        549  GCGC                 G^CGC
             1478  GCGC                
MamI          217  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1093  GATGATGATC          
NspI         1250  ACATGC               [GA]CATG^[CT]
             1471  ACATGT              
PleI          667  GACTC                GAGTC(4/5)
             1521  GAGTC               
XmnI         1108  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1299  GAAAAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanI        
BbvI        BcnI        BfaI        BmyI        BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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