Restriction map for pF1KB9983
 (Linear) (Six-base) MAPSORT of: KIBB9983.seq  Check: 8939  from: 1  to: 1710



KIBB9983.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     364    1710 
   Size:        364    1346
 
Acc65I G'GTAC_C

Cuts at:      0     844    1710 
   Size:        844     866
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614    1710 
   Size:        614    1096
 
AflIII A'CryG_T

Cuts at:      0    1471    1710 
   Size:       1471     239
 
AgeI A'CCGG_T

Cuts at:      0     784    1710 
   Size:        784     926
 
AlwNI CAG_nnn'CTG

Cuts at:      0     238     356    1710 
   Size:        238     118    1354

  Fragments arranged by size: 

                1354     238     118
 
ApaLI G'TGCA_C

Cuts at:      0    1510    1710 
   Size:       1510     200
 
ApoI r'AATT_y

Cuts at:      0     624    1500    1710 
   Size:        624     876     210

  Fragments arranged by size: 

                 876     624     210
 
AvaI C'yCGr_G

Cuts at:      0     464    1710 
   Size:        464    1246
 
BanI G'GyrC_C

Cuts at:      0     844    1367    1477    1710 
   Size:        844     523     110     233

  Fragments arranged by size: 

                 844     523     233     110
 
BbeI G_GCGC'C

Cuts at:      0    1481    1710 
   Size:       1481     229
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     998    1710 
   Size:        998     712
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1130    1710 
   Size:       1130     580
 
BclI T'GATC_A

Cuts at:      0     945    1710 
   Size:        945     765
 
BfrBI ATG'CAT

Cuts at:      0    1332    1710 
   Size:       1332     378
 
Bme1580I G_kGCm'C

Cuts at:      0     132    1514    1710 
   Size:        132    1382     196

  Fragments arranged by size: 

                1382     196     132
 
BmgBI CAC'GTC

Cuts at:      0    1067    1710 
   Size:       1067     643
 
BmtI G_CTAG'C

Cuts at:      0    1549    1710 
   Size:       1549     161
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     497    1710 
   Size:        497    1213
 
Bpu10I CC'TnA_GC

Cuts at:      0     675    1710 
   Size:        675    1035
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     775    1603    1710 
   Size:        775     828     107

  Fragments arranged by size: 

                 828     775     107
 
BsaBI GATnn'nnATC

Cuts at:      0     221    1097    1710 
   Size:        221     876     613

  Fragments arranged by size: 

                 876     613     221
 
BsaHI Gr'CG_yC

Cuts at:      0    1478    1710 
   Size:       1478     232
 
BsaWI w'CCGG_w

Cuts at:      0     784    1086    1364    1581    1710 
   Size:        784     302     278     217     129

  Fragments arranged by size: 

                 784     302     278     217     129
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1065    1095    1710 
   Size:       1065      30     615

  Fragments arranged by size: 

                1065     615      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18     904    1073    1533    1647    1710 
   Size:         18     886     169     460     114      63

  Fragments arranged by size: 

                 886     460     169     114      63      18
 
BseYI C'CCAG_C

Cuts at:      0     911    1710 
   Size:        911     799
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     519    1710 
   Size:        519    1191
 
BsiHKAI G_wGCw'C

Cuts at:      0     823    1514    1710 
   Size:        823     691     196

  Fragments arranged by size: 

                 823     691     196
 
Bsp1286I G_dGCh'C

Cuts at:      0     132     823    1514    1710 
   Size:        132     691     691     196

  Fragments arranged by size: 

                 691     691     196     132
 
BspEI T'CCGG_A

Cuts at:      0    1086    1710 
   Size:       1086     624
 
BspHI T'CATG_A

Cuts at:      0     368    1710 
   Size:        368    1342
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     364    1216    1710 
   Size:        364     852     494

  Fragments arranged by size: 

                 852     494     364
 
BsrDI GCAATG_nn'

Cuts at:      0    1136    1710 
   Size:       1136     574
 
BsrFI r'CCGG_y

Cuts at:      0     784    1710 
   Size:        784     926
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1251    1710 
   Size:       1251     459
 
BstYI r'GATC_y

Cuts at:      0     216    1710 
   Size:        216    1494
 
Bsu36I CC'TnA_GG

Cuts at:      0    1516    1710 
   Size:       1516     194
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1318    1710 
   Size:       1318     392
 
BtsI GCAGTG_nn'

Cuts at:      0     162     507    1572    1710 
   Size:        162     345    1065     138

  Fragments arranged by size: 

                1065     345     162     138
 
ClaI AT'CG_AT

Cuts at:      0    1101    1710 
   Size:       1101     609
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1455    1490    1710 
   Size:       1455      35     220

  Fragments arranged by size: 

                1455     220      35
 
EarI CTCTTCn'nnn_

Cuts at:      0      36     675    1601    1710 
   Size:         36     639     926     109

  Fragments arranged by size: 

                 926     639     109      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614    1710 
   Size:        614    1096
 
EcoO109I rG'GnC_Cy

Cuts at:      0     984    1710 
   Size:        984     726
 
EcoRI G'AATT_C

Cuts at:      0     624    1710 
   Size:        624    1086
 
EcoRV GAT'ATC

Cuts at:      0      96    1710 
   Size:         96    1614
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1003    1630    1710 
   Size:       1003     627      80

  Fragments arranged by size: 

                1003     627      80
 
HaeII r_GCGC'y

Cuts at:      0    1481    1710 
   Size:       1481     229
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     892     924    1710 
   Size:        892      32     786

  Fragments arranged by size: 

                 892     786      32
 
HincII GTy'rAC

Cuts at:      0     666    1710 
   Size:        666    1044
 
KasI G'GCGC_C

Cuts at:      0    1477    1710 
   Size:       1477     233
 
KpnI G_GTAC'C

Cuts at:      0     848    1710 
   Size:        848     862
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     194    1228    1710 
   Size:        194    1034     482

  Fragments arranged by size: 

                1034     482     194
 
MslI CAynn'nnrTG

Cuts at:      0      89     565    1281    1710 
   Size:         89     476     716     429

  Fragments arranged by size: 

                 716     476     429      89
 
MspA1I CmG'CkG

Cuts at:      0    1292    1710 
   Size:       1292     418
 
NarI GG'CG_CC

Cuts at:      0    1478    1710 
   Size:       1478     232
 
NheI G'CTAG_C

Cuts at:      0    1545    1710 
   Size:       1545     165
 
NsiI A_TGCA'T

Cuts at:      0    1334    1710 
   Size:       1334     376
 
NspI r_CATG'y

Cuts at:      0    1254    1475    1710 
   Size:       1254     221     235

  Fragments arranged by size: 

                1254     235     221
 
PciI A'CATG_T

Cuts at:      0    1471    1710 
   Size:       1471     239
 
PfoI T'CCnGG_A

Cuts at:      0     110     538     960    1710 
   Size:        110     428     422     750

  Fragments arranged by size: 

                 750     428     422     110
 
PpuMI rG'GwC_Cy

Cuts at:      0     984    1710 
   Size:        984     726
 
SfcI C'TryA_G

Cuts at:      0    1013    1710 
   Size:       1013     697
 
SfoI GGC'GCC

Cuts at:      0    1479    1710 
   Size:       1479     231
 
SmlI C'TyrA_G

Cuts at:      0     464    1710 
   Size:        464    1246
 
SspI AAT'ATT

Cuts at:      0     300    1710 
   Size:        300    1410
 
StyI C'CwwG_G

Cuts at:      0     685    1710 
   Size:        685    1025
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     411    1710 
   Size:        411    1299
 
TatI w'GTAC_w

Cuts at:      0    1156    1710 
   Size:       1156     554
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1065    1097    1710 
   Size:       1065      32     613

  Fragments arranged by size: 

                1065     613      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1169    1710 
   Size:       1169     541
 
XbaI T'CTAG_A

Cuts at:      0     420    1710 
   Size:        420    1290
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      49    1710 
   Size:         49    1661
 
XhoI C'TCGA_G

Cuts at:      0     464    1710 
   Size:        464    1246
 
XmnI GAAnn'nnTTC

Cuts at:      0    1112    1303    1710 
   Size:       1112     191     407

  Fragments arranged by size: 

                1112     407     191
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AgeI    AlwNI    ApaLI     ApoI
     AvaI     BanI     BbeI     BbsI    BciVI     BclI    BfrBI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrFI   BstAPI    BstYI   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MslI   MspA1I
     NarI     NheI     NsiI     NspI     PciI     PfoI    PpuMI     SfcI
     SfoI     SmlI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AhdI     AleI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BanII    BbvCI     BcgI     BglI    BglII     BlpI     BmrI     BpmI
    BpuEI    BsaAI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI     FseI
     FspI    FspAI  HindIII     HpaI     MfeI     MluI     MscI     NaeI
     NcoI     NdeI   NgoMIV     NotI     NruI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     XmaI     ZraI
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