Restriction map for
pF1KB6574
(Linear) (Six-base) MAPSORT of: KIBB6574.seq Check: 5667 from: 1 to: 1203
KIBB6574.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AatII G_ACGT'C
Cuts at: 0 463 1203
Size: 463 740
AccI GT'mk_AC
Cuts at: 0 671 1203
Size: 671 532
Acc65I G'GTAC_C
Cuts at: 0 139 1203
Size: 139 1064
AclI AA'CG_TT
Cuts at: 0 67 1203
Size: 67 1136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 781 997 1203
Size: 603 178 216 206
Fragments arranged by size:
603 216 206 178
AleI CACnn'nnGTG
Cuts at: 0 227 1203
Size: 227 976
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 1203
Size: 283 920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 734 766 1203
Size: 734 32 437
Fragments arranged by size:
734 437 32
AlwNI CAG_nnn'CTG
Cuts at: 0 759 975 1203
Size: 759 216 228
Fragments arranged by size:
759 228 216
BanI G'GyrC_C
Cuts at: 0 139 685 1203
Size: 139 546 518
Fragments arranged by size:
546 518 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 181 935 1105 1203
Size: 181 754 170 98
Fragments arranged by size:
754 181 170 98
BglI GCCn_nnn'nGGC
Cuts at: 0 654 1203
Size: 654 549
BmrI ACTGGGnnnn_n'
Cuts at: 0 956 1203
Size: 956 247
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 389 1203
Size: 389 814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1203
Size: 358 845
BsaI GGTCTCn'nnnn_
Cuts at: 0 514 1203
Size: 514 689
BsaHI Gr'CG_yC
Cuts at: 0 460 1203
Size: 460 743
BsaWI w'CCGG_w
Cuts at: 0 556 1203
Size: 556 647
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 57 87 256 286 1203
Size: 57 30 169 30 917
Fragments arranged by size:
917 169 57 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 1203
Size: 622 581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1203
Size: 862 341
BsmI GAATG_Cn'
Cuts at: 0 322 613 1203
Size: 322 291 590
Fragments arranged by size:
590 322 291
BsmBI CGTCTCn'nnnn_
Cuts at: 0 451 1203
Size: 451 752
BspEI T'CCGG_A
Cuts at: 0 556 1203
Size: 556 647
BsrGI T'GTAC_A
Cuts at: 0 160 1130 1203
Size: 160 970 73
Fragments arranged by size:
970 160 73
BssHII G'CGCG_C
Cuts at: 0 20 1203
Size: 20 1183
BssSI C'ACGA_G
Cuts at: 0 25 1203
Size: 25 1178
BstEII G'GTnAC_C
Cuts at: 0 1168 1203
Size: 1168 35
BstYI r'GATC_y
Cuts at: 0 971 1203
Size: 971 232
BtsI GCAGTG_nn'
Cuts at: 0 504 1203
Size: 504 699
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 781 997 1203
Size: 603 178 216 206
Fragments arranged by size:
603 216 206 178
EcoNI CCTnn'n_nnAGG
Cuts at: 0 604 1203
Size: 604 599
EcoO109I rG'GnC_Cy
Cuts at: 0 1029 1203
Size: 1029 174
HaeII r_GCGC'y
Cuts at: 0 333 1203
Size: 333 870
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 1203
Size: 448 32 723
Fragments arranged by size:
723 448 32
HindIII A'AGCT_T
Cuts at: 0 900 1203
Size: 900 303
KpnI G_GTAC'C
Cuts at: 0 143 1203
Size: 143 1060
MslI CAynn'nnrTG
Cuts at: 0 227 1203
Size: 227 976
MspA1I CmG'CkG
Cuts at: 0 114 218 756 846 1203
Size: 114 104 538 90 357
Fragments arranged by size:
538 357 114 104 90
NdeI CA'TA_TG
Cuts at: 0 550 1203
Size: 550 653
NspI r_CATG'y
Cuts at: 0 862 956 1203
Size: 862 94 247
Fragments arranged by size:
862 247 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 50 1203
Size: 50 1153
PfoI T'CCnGG_A
Cuts at: 0 375 1203
Size: 375 828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 1203
Size: 331 32 840
Fragments arranged by size:
840 331 32
PshAI GACnn'nnGTC
Cuts at: 0 128 236 1203
Size: 128 108 967
Fragments arranged by size:
967 128 108
PstI C_TGCA'G
Cuts at: 0 250 272 1203
Size: 250 22 931
Fragments arranged by size:
931 250 22
PvuII CAG'CTG
Cuts at: 0 114 218 756 1203
Size: 114 104 538 447
Fragments arranged by size:
538 447 114 104
SexAI A'CCwGG_T
Cuts at: 0 1172 1203
Size: 1172 31
SfcI C'TryA_G
Cuts at: 0 246 268 477 1203
Size: 246 22 209 726
Fragments arranged by size:
726 246 209 22
SmlI C'TyrA_G
Cuts at: 0 337 1203
Size: 337 866
SphI G_CATG'C
Cuts at: 0 956 1203
Size: 956 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 313 1203
Size: 73 240 890
Fragments arranged by size:
890 240 73
TatI w'GTAC_w
Cuts at: 0 160 1130 1203
Size: 160 970 73
Fragments arranged by size:
970 160 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 371 403 902 934 1203
Size: 371 32 499 32 269
Fragments arranged by size:
499 371 269 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 68 1203
Size: 68 1135
ZraI GAC'GTC
Cuts at: 0 461 1203
Size: 461 742
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AleI AlfI AloI
AlwNI BanI BbsI BglI BmrI BplI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BsgI BsmI BsmBI BspEI
BsrGI BssHII BssSI BstEII BstYI BtsI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII KpnI MslI MspA1I NdeI
NspI PflMI PfoI PpiI PshAI PstI PvuII SexAI
SfcI SmlI SphI TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AflIII AgeI AhdI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaAI BsaBI
BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI MfeI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaI