Restriction map for pF1KB7961
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
FbaI        FseI        FspI        HapII       Hin1I       
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeI        MaeII       MamI        MflI        
MluI        MluNI       MroI        MscI        MspI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AlwI           82  GATCC                GGATC(4/5)
BanII         692  GAGCCC               G[GA]GC[CT]^C
BbsI          784  GAAGAC               GAAGAC(2/6)
BglI          335  GCCTCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          692  GAGCCC               G[AGT]GC[ACT]^C
BpuAI         784  GAAGAC               GAAGAC(2/6)
BsaI          539  GAGACC               GGTCTC(1/5)
BsaJI         629  CCCTGG               C^C[ACGT][ACGT]GG
BseRI         313  GAGGAG               GAGGAG(10/8)
BsgI          297  GTGCAG               GTGCAG(16/14)
BsiYI         201  CCATGTGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          201  CCATGTGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         539  GAGAC                GTCTC(1/5)
BsmFI         645  GGGAC                GGGAC(10/14)
BsmI          572  GCATTC               GAATGC(1/-1)
Bsp1286I      692  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      749  TGTACA               T^GTACA
BspMI         542  ACCTGC               ACCTGC(4/8)
BsrBI         727  GAGCGG               CCGCTC(-3/-3)
BsrDI         113  GCAATG               GCAATG(2/0)
BsrGI         749  TGTACA               T^GTACA
BssKI         630  CCTGG                ^CC[ACGT]GG
BstNI         630  CCTGG                CC^[AT]GG
CfoI          198  GCGC                 GCG^C
EarI          118  GAAGAG               CTCTTC(1/4)
EcoRII        630  CCTGG                ^CC[AT]GG
EcoT22I        68  ATGCAT               ATGCA^T
FauI          729  GCGGG                CCCGC(4/6)
HaeII         197  AGCGCC               [GA]GCGC^[CT]
HgaI          403  GACGC                GACGC(5/10)
HhaI          198  GCGC                 GCG^C
HinP1I        198  GCGC                 G^CGC
HincII        733  GTTGAC               GT[CT]^[GA]AC
HindII        733  GTTGAC               GT[CT]^[GA]AC
HphI           42  GGTGA                GGTGA(8/7)
Ksp632I       118  GAAGAG               CTCTTC(1/4)
MfeI          252  CAATTG               C^AATTG
MslI          770  CACACGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          252  CAATTG               C^AATTG
MvaI          630  CCTGG                CC^[AT]GG
NdeI          674  CATATG               CA^TATG
NlaIV         215  GGCTCC               GG[ACGT]^[ACGT]CC
NsiI           68  ATGCAT               ATGCA^T
PshAI         168  GACATCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          289  CTGCAG               CTGCA^G
ScaI          122  AGTACT               AGT^ACT
ScrFI         630  CCTGG                CC^[ACGT]GG
SfcI          289  CTGCAG               C^T[GA][CT]AG
SspBI         749  TGTACA               T^GTACA
TaqI          323  TCGA                 T^CGA
TfiI          442  GATTC                G^A[AT]TC
TspRI         282  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       323  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BpmI          217  CTCCAG               CTGGAG(16/14)
              364  CTCCAG              
Cac8I         287  GCCTGC               GC[ACGT]^[ACGT]GC
              331  GCTGGC              
DdeI          194  CTAAG                C^T[ACGT]AG
              697  CTGAG               
DpnII          82  GATC                 ^GATC
              103  GATC                
MboI           82  GATC                 ^GATC
              103  GATC                
MseI          398  TTAA                 T^TAA
              617  TTAA                
MspA1I        292  CAGCTG               C[AC]G^C[GT]G
              329  CAGCTG              
MwoI            3  GCCATCTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              335  GCCTCTGCGGC         
NdeII          82  GATC                 ^GATC
              103  GATC                
NlaIII        202  CATG                 CATG^
              303  CATG                
PleI           37  GACTC                GAGTC(4/5)
              157  GACTC               
PvuII         292  CAGCTG               CAG^CTG
              329  CAGCTG              
Sau3AI         82  GATC                 ^GATC
              103  GATC                
Tru9I         398  TTAA                 T^TAA
              617  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BsrI        BstF5I      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeIII      MboII       
MnlI        RsaI        SfaNI       TseI        Tsp45I      
Tsp509I     
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