Restriction map for pF1KB5673
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          579  GTCTAC               GT^[AC][GT]AC
AciI          646  CCGC                 CCGC(-3/-1)
AocI           58  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1072  AGCGCT               AGC^GCT
AvaI          827  CTCGGG               C^[CT]CG[GA]G
BanII        1063  GAGCCC               G[GA]GC[CT]^C
BbsI           70  GAAGAC               GAAGAC(2/6)
BbvCI         459  CCTCAGC              CCTCAGC(-5/-2)
BbvI         1339  GCTGC                GCAGC(8/12)
BcnI          755  CCCGG                CC^[GC]GG
BpmI          344  CTCCAG               CTGGAG(16/14)
BpuAI          70  GAAGAC               GAAGAC(2/6)
BsaAI        1642  TACGTG               [CT]AC^GT[GA]
BsaBI        1328  GATTCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         442  CTCCTC               GAGGAG(10/8)
BsmFI          96  GTCCC                GGGAC(10/14)
BsoBI         827  CTCGGG               C^[CT]CG[GA]G
BspHI         680  TCATGA               T^CATGA
BspLU11I      428  ACATGT               A^CATGT
BsrDI        1529  GCAATG               GCAATG(2/0)
BsrFI          32  ACCGGC               [GA]^CCGG[CT]
BssSI        1076  CTCGTG               CACGAG(-5/-1)
Bsu36I         58  CCTGAGG              CC^T[ACGT]AGG
Cac8I        1160  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr10I         32  ACCGGC               [GA]^CCGG[CT]
DsaI          666  CCATGG               C^C[GA][CT]GG
EaeI         1618  CGGCCA               [CT]^GGCC[GA]
Eco47III     1072  AGCGCT               AGC^GCT
Eco81I         58  CCTGAGG              CC^T[ACGT]AGG
Fnu4HI       1339  GCTGC                GC^[ACGT]GC
ItaI         1339  GCTGC                GC^[ACGT]GC
MamI         1328  GATTCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         1100  TTAA                 T^TAA
MspA1I        646  CCGCTG               C[AC]G^C[GT]G
NciI          755  CCCGG                CC^[GC]GG
NcoI          666  CCATGG               C^CATGG
NdeI          719  CATATG               CA^TATG
NlaIV        1062  GGAGCC               GG[ACGT]^[ACGT]CC
NspI          428  ACATGT               [GA]CATG^[CT]
RcaI          680  TCATGA               T^CATGA
SexAI         559  ACCTGGT              A^CC[AT]GGT
SfaNI         253  GCATC                GCATC(5/9)
Tru9I        1100  TTAA                 T^TAA
TseI         1339  GCTGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI          538  AAATTT               [GA]^AATT[CT]
              837  AAATTC              
AflIII        428  ACATGT               A^C[GA][CT]GT
             1643  ACGTGT              
AlwI          651  GATCC                GGATC(4/5)
              566  GGATC               
ApoI          538  AAATTT               [GA]^AATT[CT]
              837  AAATTC              
BsmAI         103  GAGAC                GTCTC(1/5)
               21  GTCTC               
BstF5I        643  CATCC                GGATG(2/0)
             1202  GGATG               
BstXI        1598  CCACATTTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1621  CCACGTTCCTGG        
EcoT14I       666  CCATGG               C^C[AT][AT]GG
             1388  CCTTGG              
FokI          643  CATCC                GGATG(9/13)
             1202  GGATG               
HaeII         374  GGCGCT               [GA]GCGC^[CT]
             1072  AGCGCT              
HincII        159  GTTGAC               GT[CT]^[GA]AC
              880  GTCAAC              
HindII        159  GTTGAC               GT[CT]^[GA]AC
              880  GTCAAC              
HphI            7  GGTGA                GGTGA(8/7)
             1195  GGTGA               
MslI          214  CACTTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1378  CACGAATATG          
PleI           94  GAGTC                GAGTC(4/5)
             1535  GAGTC               
SapI         1422  GCTCTTC              GCTCTTC(1/4)
             1431  GCTCTTC             
StyI          666  CCATGG               C^C[AT][AT]GG
             1388  CCTTGG              
TaqI          120  TCGA                 T^CGA
              773  TCGA                
TthHB8I       120  TCGA                 T^CGA
              773  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AfaI        AluI        AspHI       AvaII       BfaI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstNI       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NdeII       NlaIII      RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        SmlI        TfiI        
Tsp45I      Tsp509I     TspRI       
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