Restriction map for pF1KB5673
 (Linear) (Six-base) MAPSORT of: KIBB5673.seq  Check: 4490  from: 1  to: 1650



KIBB5673.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     580    1650 
   Size:        580    1070
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1053    1650 
   Size:       1053     597
 
AfeI AGC'GCT

Cuts at:      0    1074    1650 
   Size:       1074     576
 
AflIII A'CryG_T

Cuts at:      0     428    1643    1650 
   Size:        428    1215       7

  Fragments arranged by size: 

                1215     428       7
 
ApoI r'AATT_y

Cuts at:      0     538     837    1650 
   Size:        538     299     813

  Fragments arranged by size: 

                 813     538     299
 
AvaI C'yCGr_G

Cuts at:      0     827    1650 
   Size:        827     823
 
BanII G_rGCy'C

Cuts at:      0    1067    1650 
   Size:       1067     583
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      77    1650 
   Size:         77    1573
 
BbvCI CC'TCA_GC

Cuts at:      0     460    1650 
   Size:        460    1190
 
Bme1580I G_kGCm'C

Cuts at:      0    1038    1650 
   Size:       1038     612
 
BmgBI CAC'GTC

Cuts at:      0    1232    1573    1650 
   Size:       1232     341      77

  Fragments arranged by size: 

                1232     341      77
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     359    1558    1650 
   Size:        359    1199      92

  Fragments arranged by size: 

                1199     359      92
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329    1650 
   Size:        329    1321
 
Bpu10I CC'TnA_GC

Cuts at:      0     460     845    1650 
   Size:        460     385     805

  Fragments arranged by size: 

                 805     460     385
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526    1422    1541    1650 
   Size:        526     896     119     109

  Fragments arranged by size: 

                 896     526     119     109
 
BsaAI yAC'GTr

Cuts at:      0    1644    1650 
   Size:       1644       6
 
BsaBI GATnn'nnATC

Cuts at:      0    1332    1650 
   Size:       1332     318
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     433    1650 
   Size:        433    1217
 
BsiHKAI G_wGCw'C

Cuts at:      0     342    1424    1433    1650 
   Size:        342    1082       9     217

  Fragments arranged by size: 

                1082     342     217       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     342    1038    1067    1424    1433    1650 
   Size:        342     696      29     357       9     217

  Fragments arranged by size: 

                 696     357     342     217      29       9
 
BspHI T'CATG_A

Cuts at:      0     680    1650 
   Size:        680     970
 
BsrDI GCAATG_nn'

Cuts at:      0    1536    1650 
   Size:       1536     114
 
BsrFI r'CCGG_y

Cuts at:      0      32    1650 
   Size:         32    1618
 
BssSI C'ACGA_G

Cuts at:      0    1076    1650 
   Size:       1076     574
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1605    1628    1650 
   Size:       1605      23      22

  Fragments arranged by size: 

                1605      23      22
 
Bsu36I CC'TnA_GG

Cuts at:      0      59    1650 
   Size:         59    1591
 
BtgI C'CryG_G

Cuts at:      0     666    1650 
   Size:        666     984
 
BtsI GCAGTG_nn'

Cuts at:      0     739    1155    1650 
   Size:        739     416     495

  Fragments arranged by size: 

                 739     495     416
 
EaeI y'GGCC_r

Cuts at:      0    1618    1650 
   Size:       1618      32
 
EarI CTCTTCn'nnn_

Cuts at:      0       5     667    1135    1429    1438    1650 
   Size:          5     662     468     294       9     212

  Fragments arranged by size: 

                 662     468     294     212       9       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329    1053    1650 
   Size:        329     724     597

  Fragments arranged by size: 

                 724     597     329
 
HaeII r_GCGC'y

Cuts at:      0     378    1076    1650 
   Size:        378     698     574

  Fragments arranged by size: 

                 698     574     378
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     627     642     659     674    1650 
   Size:          4      32     591      15      17      15     976

  Fragments arranged by size: 

                 976     591      32      17      15      15       4
 
HincII GTy'rAC

Cuts at:      0     161     882    1650 
   Size:        161     721     768

  Fragments arranged by size: 

                 768     721     161
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1265    1459    1650 
   Size:       1265     194     191

  Fragments arranged by size: 

                1265     194     191
 
MslI CAynn'nnrTG

Cuts at:      0     218    1382    1650 
   Size:        218    1164     268

  Fragments arranged by size: 

                1164     268     218
 
MspA1I CmG'CkG

Cuts at:      0     648    1650 
   Size:        648    1002
 
NcoI C'CATG_G

Cuts at:      0     666    1650 
   Size:        666     984
 
NdeI CA'TA_TG

Cuts at:      0     720    1650 
   Size:        720     930
 
NspI r_CATG'y

Cuts at:      0     432    1650 
   Size:        432    1218
 
PciI A'CATG_T

Cuts at:      0     428    1650 
   Size:        428    1222
 
PfoI T'CCnGG_A

Cuts at:      0    1627    1650 
   Size:       1627      23
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1429    1438    1650 
   Size:       1429       9     212

  Fragments arranged by size: 

                1429     212       9
 
SexAI A'CCwGG_T

Cuts at:      0     559    1650 
   Size:        559    1091
 
SfcI C'TryA_G

Cuts at:      0     627     875    1370    1650 
   Size:        627     248     495     280

  Fragments arranged by size: 

                 627     495     280     248
 
SmlI C'TyrA_G

Cuts at:      0     505    1401    1556    1650 
   Size:        505     896     155      94

  Fragments arranged by size: 

                 896     505     155      94
 
StyI C'CwwG_G

Cuts at:      0     666    1388    1650 
   Size:        666     722     262

  Fragments arranged by size: 

                 722     666     262
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     260    1650 
   Size:        260    1390
 
TatI w'GTAC_w

Cuts at:      0     893    1010    1374    1650 
   Size:        893     117     364     276

  Fragments arranged by size: 

                 893     364     276     117
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     485     517    1650 
   Size:        485      32    1133

  Fragments arranged by size: 

                1133     485      32
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     ApoI     AvaI    BanII     BbsI
    BbvCI Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BseRI  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrFI    BssSI
    BstXI   Bsu36I     BtgI     BtsI     EaeI     EarI  Eco57MI    HaeII
    Hin4I   HincII     MmeI     MslI   MspA1I     NcoI     NdeI     NspI
     PciI     PfoI     SapI    SexAI     SfcI     SmlI     StyI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BsaI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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