Restriction map for pF1KB4961
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AfaI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaI        BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmI        Bsp1286I    Bsp1407I    BspDI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HhaI        HinP1I      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MfeI        MluI        MluNI       
MscI        MslI        MspA1I      MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII        242  TCCGGA               T^CCGGA
AcyI          271  GACGCC               G[GA]^CG[CT]C
AflIII        147  ACGTGT               A^C[GA][CT]GT
AlwI          178  GGATC                GGATC(4/5)
AvaII         169  GGACC                G^G[AT]CC
BbvI          490  GCAGC                GCAGC(8/12)
BclI           26  TGATCA               T^GATCA
BsaHI         271  GACGCC               G[GA]^CG[CT]C
BsaWI         242  TCCGGA               [AT]^CCGG[AT]
BseAI         242  TCCGGA               T^CCGGA
BsgI          488  GTGCAG               GTGCAG(16/14)
BsmBI         269  GAGACG               CGTCTC(1/5)
BspEI         242  TCCGGA               T^CCGGA
BspHI         542  TCATGA               T^CATGA
BsrBI         235  GAGCGG               CCGCTC(-3/-3)
BssKI          36  CCTGG                ^CC[ACGT]GG
BstNI          36  CCTGG                CC^[AT]GG
BstYI         178  GGATCT               [GA]^GATC[CT]
DraI          196  TTTAAA               TTT^AAA
DsaI          275  CCATGG               C^C[GA][CT]GG
EcoRII         36  CCTGG                ^CC[AT]GG
EcoT14I       275  CCATGG               C^C[AT][AT]GG
FauI          226  GCGGG                CCCGC(4/6)
FbaI           26  TGATCA               T^GATCA
Fnu4HI        490  GCAGC                GC^[ACGT]GC
HaeIII         39  GGCC                 GG^CC
HapII         243  CCGG                 C^CGG
HgaI          271  GACGC                GACGC(5/10)
Hin1I         271  GACGCC               G[GA]^CG[CT]C
HincII        308  GTCAAC               GT[CT]^[GA]AC
HindII        308  GTCAAC               GT[CT]^[GA]AC
HpaII         243  CCGG                 C^CGG
ItaI          490  GCAGC                GC^[ACGT]GC
MaeII         147  ACGT                 A^CGT
MflI          178  GGATCT               [GA]^GATC[CT]
MroI          242  TCCGGA               T^CCGGA
MspI          243  CCGG                 C^CGG
MvaI           36  CCTGG                CC^[AT]GG
MwoI          204  GCATCTTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          275  CCATGG               C^CATGG
NlaIV         239  GGTTCC               GG[ACGT]^[ACGT]CC
PleI          246  GAGTC                GAGTC(4/5)
RcaI          542  TCATGA               T^CATGA
ScrFI          36  CCTGG                CC^[ACGT]GG
SfaNI         204  GCATC                GCATC(5/9)
StyI          275  CCATGG               C^C[AT][AT]GG
TfiI           49  GATTC                G^A[AT]TC
TseI          490  GCAGC                G^C[AT]GC
XhoII         178  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI          226  GCGG                 CCGC(-3/-1)
              237  GCGG                
AlwNI         330  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
              491  CAGCAACTG           
AseI          288  ATTAAT               AT^TAAT
              387  ATTAAT              
AsnI          288  ATTAAT               AT^TAAT
              387  ATTAAT              
AvaI           43  CTCGGG               C^[CT]CG[GA]G
              443  CTCGGG              
BbsI           63  GAAGAC               GAAGAC(2/6)
              570  GAAGAC              
BpuAI          63  GAAGAC               GAAGAC(2/6)
              570  GAAGAC              
BsaJI          42  CCTCGG               C^C[ACGT][ACGT]GG
              275  CCATGG              
BsmFI         218  GGGAC                GGGAC(10/14)
              228  GGGAC               
BsoBI          43  CTCGGG               C^[CT]CG[GA]G
              443  CTCGGG              
BstF5I        338  GGATG                GGATG(2/0)
              618  GGATG               
Cac8I         351  GCAAGC               GC[ACGT]^[ACGT]GC
              438  GCAAGC              
Cfr13I         39  GGCCC                G^G[ACGT]CC
              169  GGACC               
FokI          338  GGATG                GGATG(9/13)
              618  GGATG               
HinfI          49  GATTC                G^A[ACGT]TC
              246  GAGTC               
HphI          251  TCACC                GGTGA(8/7)
              302  TCACC               
NlaIII        276  CATG                 CATG^
              543  CATG                
PshBI         288  ATTAAT               AT^TAAT
              387  ATTAAT              
Sau96I         39  GGCCC                G^G[ACGT]CC
              169  GGACC               
SfcI          119  CTACAG               C^T[GA][CT]AG
              518  CTGTAG              


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AcsI        AluI        ApoI        BsmAI       BsrI        
DpnII       MboI        MboII       MnlI        MseI        
NdeII       Sau3AI      Tru9I       Tsp509I     TspRI       
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