Restriction map for
pF1KB3236
(Linear) (Six-base) MAPSORT of: KIBB3236.seq Check: 5690 from: 1 to: 1716
KIBB3236.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1700 1716
Size: 386 1314 16
Fragments arranged by size:
1314 386 16
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1272 1716
Size: 1272 444
AlwNI CAG_nnn'CTG
Cuts at: 0 823 1716
Size: 823 893
ApoI r'AATT_y
Cuts at: 0 180 286 679 1166 1716
Size: 180 106 393 487 550
Fragments arranged by size:
550 487 393 180 106
AseI AT'TA_AT
Cuts at: 0 1015 1716
Size: 1015 701
AvrII C'CTAG_G
Cuts at: 0 918 1716
Size: 918 798
BanII G_rGCy'C
Cuts at: 0 8 1716
Size: 8 1708
BbvCI CC'TCA_GC
Cuts at: 0 1445 1716
Size: 1445 271
BclI T'GATC_A
Cuts at: 0 879 1716
Size: 879 837
BfrBI ATG'CAT
Cuts at: 0 415 754 1561 1716
Size: 415 339 807 155
Fragments arranged by size:
807 415 339 155
BlpI GC'TnA_GC
Cuts at: 0 1457 1716
Size: 1457 259
BmgBI CAC'GTC
Cuts at: 0 1030 1279 1716
Size: 1030 249 437
Fragments arranged by size:
1030 437 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1397 1716
Size: 926 471 319
Fragments arranged by size:
926 471 319
Bpu10I CC'TnA_GC
Cuts at: 0 1445 1716
Size: 1445 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1716
Size: 539 1177
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 473 844 1716
Size: 370 103 371 872
Fragments arranged by size:
872 371 370 103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 613 1716
Size: 613 1103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1708 1716
Size: 1708 8
BsiEI CG_ry'CG
Cuts at: 0 387 1716
Size: 387 1329
BsmI GAATG_Cn'
Cuts at: 0 756 1153 1716
Size: 756 397 563
Fragments arranged by size:
756 563 397
BsmBI CGTCTCn'nnnn_
Cuts at: 0 766 1716
Size: 766 950
Bsp1286I G_dGCh'C
Cuts at: 0 8 1716
Size: 8 1708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 826 1716
Size: 56 770 890
Fragments arranged by size:
890 770 56
BsrBI CCG'CTC
Cuts at: 0 1622 1716
Size: 1622 94
BstAPI GCAn_nnn'nTGC
Cuts at: 0 716 826 1288 1716
Size: 716 110 462 428
Fragments arranged by size:
716 462 428 110
BstBI TT'CG_AA
Cuts at: 0 855 1716
Size: 855 861
BstXI CCAn_nnnn'nTGG
Cuts at: 0 904 1716
Size: 904 812
BstYI r'GATC_y
Cuts at: 0 155 228 612 1716
Size: 155 73 384 1104
Fragments arranged by size:
1104 384 155 73
BtsI GCAGTG_nn'
Cuts at: 0 1261 1716
Size: 1261 455
EaeI y'GGCC_r
Cuts at: 0 418 1347 1716
Size: 418 929 369
Fragments arranged by size:
929 418 369
EarI CTCTTCn'nnn_
Cuts at: 0 92 636 857 1325 1478 1676 1716
Size: 92 544 221 468 153 198 40
Fragments arranged by size:
544 468 221 198 153 92 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 926 1397 1700 1716
Size: 386 540 471 303 16
Fragments arranged by size:
540 471 386 303 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 73 1349 1716
Size: 73 1276 367
Fragments arranged by size:
1276 367 73
EcoRI G'AATT_C
Cuts at: 0 679 1716
Size: 679 1037
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 1326 1358 1716
Size: 537 32 757 32 358
Fragments arranged by size:
757 537 358 32 32
HincII GTy'rAC
Cuts at: 0 1710 1716
Size: 1710 6
MfeI C'AATT_G
Cuts at: 0 109 1142 1716
Size: 109 1033 574
Fragments arranged by size:
1033 574 109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 1716
Size: 1249 467
MscI TGG'CCA
Cuts at: 0 420 1349 1716
Size: 420 929 367
Fragments arranged by size:
929 420 367
MslI CAynn'nnrTG
Cuts at: 0 445 508 1070 1716
Size: 445 63 562 646
Fragments arranged by size:
646 562 445 63
NsiI A_TGCA'T
Cuts at: 0 417 756 1563 1716
Size: 417 339 807 153
Fragments arranged by size:
807 417 339 153
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1154 1186 1716
Size: 1154 32 530
Fragments arranged by size:
1154 530 32
PstI C_TGCA'G
Cuts at: 0 1251 1716
Size: 1251 465
PvuI CG_AT'CG
Cuts at: 0 387 1716
Size: 387 1329
SapI GCTCTTCn'nnn_
Cuts at: 0 857 1716
Size: 857 859
ScaI AGT'ACT
Cuts at: 0 1638 1716
Size: 1638 78
SfcI C'TryA_G
Cuts at: 0 1247 1716
Size: 1247 469
SmlI C'TyrA_G
Cuts at: 0 554 1716
Size: 554 1162
SspI AAT'ATT
Cuts at: 0 1428 1716
Size: 1428 288
StyI C'CwwG_G
Cuts at: 0 483 897 918 947 1271 1350 1716
Size: 483 414 21 29 324 79 366
Fragments arranged by size:
483 414 366 324 79 29 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 1716
Size: 46 1670
TatI w'GTAC_w
Cuts at: 0 1636 1716
Size: 1636 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1572 1604 1716
Size: 1572 32 112
Fragments arranged by size:
1572 112 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 1716
Size: 318 1398
XmnI GAAnn'nnTTC
Cuts at: 0 1166 1716
Size: 1166 550
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AseI AvrII BanII BbvCI
BclI BfrBI BlpI BmgBI BpmI Bpu10I BpuEI BsaI
BseRI BsgI BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI
BstAPI BstBI BstXI BstYI BtsI EaeI EarI Eco57MI
EcoNI EcoRI Hin4I HincII MfeI MmeI MscI MslI
NsiI PpiI PstI PvuI SapI ScaI SfcI SmlI
SspI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI
AvaI BaeI BamHI BanI BbeI BbsI BcgI BciVI
BglI BglII Bme1580I BmrI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiHKAI BsiWI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XbaI XhoI XmaI ZraI