Restriction map for pF1KB7990
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        Alw44I      
AlwI        AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiHKAI     BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FseI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     XbaI        XcmI        
XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI          103  AAATTC               [GA]^AATT[CT]
AcyI          431  GACGCC               G[GA]^CG[CT]C
AfaI            3  GTAC                 GT^AC
AhdI           28  GACACCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       725  AGCGCT               AGC^GCT
ApoI          103  AAATTC               [GA]^AATT[CT]
AspEI          28  GACACCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI          841  GTCTTC               GAAGAC(2/6)
BfaI          854  CTAG                 C^TAG
BlpI          511  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      511  GCTGAGC              GC^T[ACGT]AGC
BpuAI         841  GTCTTC               GAAGAC(2/6)
BsaHI         431  GACGCC               G[GA]^CG[CT]C
BsiEI         595  CGATCG               CG[GA][CT]^CG
BsmI          136  GCATTC               GAATGC(1/-1)
BsrDI         750  CATTGC               GCAATG(2/0)
BsrI          565  CCAGT                ACTGG(1/-1)
BstAPI        618  GCAGCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        798  GGTGACC              G^GT[ACGT]ACC
BstPI         798  GGTGACC              G^GT[ACGT]ACC
BstXI         962  CCACTCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         511  GCTGAGC              GC^T[ACGT]AGC
DraII         173  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          326  CCGTGG               C^C[GA][CT]GG
EaeI           93  CGGCCA               [CT]^GGCC[GA]
Eam1105I       28  GACACCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      725  AGCGCT               AGC^GCT
EcoO109I      173  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       798  GGTGACC              G^GT[ACGT]ACC
EcoT14I       902  CCAAGG               C^C[AT][AT]GG
Hin1I         431  GACGCC               G[GA]^CG[CT]C
MaeI          854  CTAG                 C^TAG
PpuMI         173  AGGACCT              [GA]G^G[AT]CC[CT]
PvuI          595  CGATCG               CGAT^CG
PvuII         352  CAGCTG               CAG^CTG
RsaI            3  GTAC                 GT^AC
StuI          492  AGGCCT               AGG^CCT
StyI          902  CCAAGG               C^C[AT][AT]GG
TaqI          832  TCGA                 T^CGA
TfiI          892  GAATC                G^A[AT]TC
Tsp509I       104  AATT                 ^AATT
TthHB8I       832  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         172  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              605  CAGCCCCTG           
AvaII         174  GGACC                G^G[AT]CC
              933  GGACC               
AviII          60  TGCGCA               TGC^GCA
              626  TGCGCA              
BanI          319  GGCACC               G^G[CT][GA]CC
              826  GGCACC              
BanII         670  GAGCCC               G[GA]GC[CT]^C
              775  GAGCCC              
BclI          203  TGATCA               T^GATCA
              305  TGATCA              
BsgI          781  CTGCAC               GTGCAG(16/14)
              190  GTGCAG              
BsmAI         463  GAGAC                GTCTC(1/5)
              357  GTCTC               
BspMI         176  ACCTGC               ACCTGC(4/8)
              810  ACCTGC              
DdeI          126  CTCAG                C^T[ACGT]AG
              512  CTGAG               
EarI          364  GAAGAG               CTCTTC(1/4)
              679  GAAGAG              
FbaI          203  TGATCA               T^GATCA
              305  TGATCA              
FspI           60  TGCGCA               TGC^GCA
              626  TGCGCA              
Ksp632I       364  GAAGAG               CTCTTC(1/4)
              679  GAAGAG              
PflMI         292  CCAAGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              604  CCAGCCCCTGG         
SfaNI         383  GCATC                GCATC(5/9)
              880  GCATC               
SmaI           16  CCCGGG               CCC^GGG
               38  CCCGGG              
Van91I        292  CCAAGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              604  CCAGCCCCTGG         
XmaCI          16  CCCGGG               C^CCGGG
               38  CCCGGG              
XmaI           16  CCCGGG               C^CCGGG
               38  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        AvaI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DpnII       EcoNI       EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        Sau3AI      Sau96I      ScrFI       SfcI        
TseI        Tsp45I      TspRI       
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