Restriction map for pF1KB7990
 (Linear) (Six-base) MAPSORT of: KIBB7990.seq  Check: 5176  from: 1  to: 978



KIBB7990.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     819     978 
   Size:        819     159
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     978 
   Size:        383     595
 
AfeI AGC'GCT

Cuts at:      0     727     978 
   Size:        727     251
 
AhdI GACnn_n'nnGTC

Cuts at:      0      33     978 
   Size:         33     945
 
AleI CACnn'nnGTG

Cuts at:      0     325     978 
   Size:        325     653
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     435     978 
   Size:        435     543
 
AlwNI CAG_nnn'CTG

Cuts at:      0     177     610     978 
   Size:        177     433     368

  Fragments arranged by size: 

                 433     368     177
 
ApoI r'AATT_y

Cuts at:      0     103     978 
   Size:        103     875
 
AvaI C'yCGr_G

Cuts at:      0      16      38     583     978 
   Size:         16      22     545     395

  Fragments arranged by size: 

                 545     395      22      16
 
BanI G'GyrC_C

Cuts at:      0     319     826     978 
   Size:        319     507     152

  Fragments arranged by size: 

                 507     319     152
 
BanII G_rGCy'C

Cuts at:      0     674     779     978 
   Size:        674     105     199

  Fragments arranged by size: 

                 674     199     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     834     978 
   Size:        834     144
 
BclI T'GATC_A

Cuts at:      0     203     305     978 
   Size:        203     102     673

  Fragments arranged by size: 

                 673     203     102
 
BlpI GC'TnA_GC

Cuts at:      0     512     978 
   Size:        512     466
 
Bme1580I G_kGCm'C

Cuts at:      0     829     978 
   Size:        829     149
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     559     978 
   Size:        559     419
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      93     978 
   Size:         93     885
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     496     559     883     978 
   Size:         63     433      63     324      95

  Fragments arranged by size: 

                 433     324      95      63      63
 
Bpu10I CC'TnA_GC

Cuts at:      0     126     978 
   Size:        126     852
 
BsaHI Gr'CG_yC

Cuts at:      0     432     978 
   Size:        432     546
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     472     502     532     562     856     886     978 
   Size:        472      30      30      30     294      30      92

  Fragments arranged by size: 

                 472     294      92      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     354     382     385     484     493     496     601     787 
   Size:        354      28       3      99       9       3     105     186

Cuts at:    787     978 
   Size:        191

  Fragments arranged by size: 

                 354     191     186     105      99      28       9       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     603     722     754     978 
   Size:        603     119      32     224

  Fragments arranged by size: 

                 603     224     119      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     766     978 
   Size:        211     555     212

  Fragments arranged by size: 

                 555     212     211
 
BsiEI CG_ry'CG

Cuts at:      0     598     978 
   Size:        598     380
 
BsmI GAATG_Cn'

Cuts at:      0     136     978 
   Size:        136     842
 
Bsp1286I G_dGCh'C

Cuts at:      0     674     779     829     978 
   Size:        674     105      50     149

  Fragments arranged by size: 

                 674     149     105      50
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     185     819     978 
   Size:        185     634     159

  Fragments arranged by size: 

                 634     185     159
 
BsrDI GCAATG_nn'

Cuts at:      0     749     978 
   Size:        749     229
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     624     978 
   Size:        624     354
 
BstEII G'GTnAC_C

Cuts at:      0     798     978 
   Size:        798     180
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     969     978 
   Size:        969       9
 
BtgI C'CryG_G

Cuts at:      0     326     978 
   Size:        326     652
 
BtsI GCAGTG_nn'

Cuts at:      0     138     187     636     978 
   Size:        138      49     449     342

  Fragments arranged by size: 

                 449     342     138      49
 
EaeI y'GGCC_r

Cuts at:      0      93     978 
   Size:         93     885
 
EarI CTCTTCn'nnn_

Cuts at:      0     359     674     978 
   Size:        359     315     304

  Fragments arranged by size: 

                 359     315     304
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     475     978 
   Size:        475     503
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     383     496     559     883     978 
   Size:         63     320     113      63     324      95

  Fragments arranged by size: 

                 324     320     113      95      63      63
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     125     499     679     865     978 
   Size:        125     374     180     186     113

  Fragments arranged by size: 

                 374     186     180     125     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0     174     978 
   Size:        174     804
 
FspI TGC'GCA

Cuts at:      0      62     628     978 
   Size:         62     566     350

  Fragments arranged by size: 

                 566     350      62
 
HaeII r_GCGC'y

Cuts at:      0     594     653     729     978 
   Size:        594      59      76     249

  Fragments arranged by size: 

                 594     249      76      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      19      51     340     372     472     504     580     612 
   Size:         19      32     289      32     100      32      76      32

Cuts at:    612     676     708     856     888     925     957     978 
   Size:         64      32     148      32      37      32      21

  Fragments arranged by size: 

                 289     148     100      76      64      37      32      32
                  32      32      32      32      32      21      19
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     978 
   Size:        242     736
 
MslI CAynn'nnrTG

Cuts at:      0     211     325     550     796     978 
   Size:        211     114     225     246     182

  Fragments arranged by size: 

                 246     225     211     182     114
 
MspA1I CmG'CkG

Cuts at:      0      50      83     354     929     978 
   Size:         50      33     271     575      49

  Fragments arranged by size: 

                 575     271      50      49      33
 
PasI CC'CwG_GG

Cuts at:      0     610     978 
   Size:        610     368
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     298     610     978 
   Size:        298     312     368

  Fragments arranged by size: 

                 368     312     298
 
PfoI T'CCnGG_A

Cuts at:      0     860     978 
   Size:        860     118
 
PpuMI rG'GwC_Cy

Cuts at:      0     174     978 
   Size:        174     804
 
PstI C_TGCA'G

Cuts at:      0     182     414     458     500     521     620     978 
   Size:        182     232      44      42      21      99     358

  Fragments arranged by size: 

                 358     232     182      99      44      42      21
 
PvuI CG_AT'CG

Cuts at:      0     598     978 
   Size:        598     380
 
PvuII CAG'CTG

Cuts at:      0     354     978 
   Size:        354     624
 
SfcI C'TryA_G

Cuts at:      0     178     243     410     454     496     517     616     653 
   Size:        178      65     167      44      42      21      99      37

Cuts at:    653     978 
   Size:        325

  Fragments arranged by size: 

                 325     178     167      99      65      44      42      37
                  21
 
SmaI CCC'GGG

Cuts at:      0      18      40     978 
   Size:         18      22     938

  Fragments arranged by size: 

                 938      22      18
 
StuI AGG'CCT

Cuts at:      0     494     978 
   Size:        494     484
 
StyI C'CwwG_G

Cuts at:      0     902     978 
   Size:        902      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      55      87     166     198     978 
   Size:         55      32      79      32     780

  Fragments arranged by size: 

                 780      79      55      32      32
 
XmaI C'CCGG_G

Cuts at:      0      16      38     978 
   Size:         16      22     940

  Fragments arranged by size: 

                 940      22      16
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AhdI     AleI     AlfI    AlwNI     ApoI
     AvaI     BanI    BanII     BbsI     BclI     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI     BsmI Bsp1286I    BspMI    BsrDI   BstAPI   BstEII    BstXI
     BtgI     BtsI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FspI    HaeII    Hin4I     MmeI     MslI   MspA1I     PasI    PflMI
     PfoI    PpuMI     PstI     PvuI    PvuII     SfcI     SmaI     StuI
     StyI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII    BmgBI
     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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