(Circular) (Six-base) MAPSORT of: pF1KE2494.seq Check: 8092 from: 1 to: 4283 pF1KE2494 4283 bp With 182 enzymes: SgfI * December 2, 2013 11:07 .. AccI GT'mk_AC Cuts at: 416 1321 416 Size: 905 3378 Acc65I G'GTAC_C Cuts at: 1299 1299 Size: 4283 AclI AA'CG_TT Cuts at: 4123 4123 Size: 4283 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 482 781 910 2108 2540 3287 3555 482 Size: 299 129 1198 432 747 268 1210 Fragments arranged by size: 1210 1198 747 432 299 268 129 AflIII A'CryG_T Cuts at: 1635 2739 3716 1635 Size: 1104 977 2202 Fragments arranged by size: 2202 1104 977 AgeI A'CCGG_T Cuts at: 2671 2671 Size: 4283 AleI CACnn'nnGTG Cuts at: 535 535 Size: 4283 AlwNI CAG_nnn'CTG Cuts at: 1588 3155 1588 Size: 1567 2716 ApaI G_GGCC'C Cuts at: 116 116 Size: 4283 ApaLI G'TGCA_C Cuts at: 3053 3053 Size: 4283 ApoI r'AATT_y Cuts at: 1287 3636 3892 1287 Size: 2349 256 1678 Fragments arranged by size: 2349 1678 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4283 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4283 AvaI C'yCGr_G Cuts at: 313 1303 313 Size: 990 3293 BamHI G'GATC_C Cuts at: 1308 1308 Size: 4283 BanI G'GyrC_C Cuts at: 1299 1713 1961 1996 1299 Size: 414 248 35 3586 Fragments arranged by size: 3586 414 248 35 BanII G_rGCy'C Cuts at: 116 1297 2327 116 Size: 1181 1030 2072 Fragments arranged by size: 2072 1181 1030 BbeI G_GCGC'C Cuts at: 1717 1965 1717 Size: 248 4035 BbsI GAAGACnn'nnnn_ Cuts at: 395 1099 395 Size: 704 3579 BbvCI CC'TCA_GC Cuts at: 692 1199 692 Size: 507 3776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 505 539 505 Size: 34 4249 BciVI GTATCCnnnnn_n' Cuts at: 2181 2942 4283 2181 Size: 761 1341 2181 Fragments arranged by size: 2181 1341 761 BclI T'GATC_A Cuts at: 236 1803 236 Size: 1567 2716 BglII A'GATC_T Cuts at: 1032 1798 3835 1032 Size: 766 2037 1480 Fragments arranged by size: 2037 1480 766 BlpI GC'TnA_GC Cuts at: 1395 1395 Size: 4283 Bme1580I G_kGCm'C Cuts at: 116 1908 2001 3057 116 Size: 1792 93 1056 1342 Fragments arranged by size: 1792 1342 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1095 1495 1576 1911 4055 1095 Size: 400 81 335 2144 1323 Fragments arranged by size: 2144 1323 400 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2650 2650 Size: 4283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 385 1228 1240 1252 385 Size: 843 12 12 3416 Fragments arranged by size: 3416 843 12 12 Bpu10I CC'TnA_GC Cuts at: 692 1199 692 Size: 507 3776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 982 1459 2338 2830 3128 982 Size: 477 879 492 298 2137 Fragments arranged by size: 2137 879 492 477 298 BsaI GGTCTCn'nnnn_ Cuts at: 904 3987 904 Size: 3083 1200 BsaAI yAC'GTr Cuts at: 1636 1636 Size: 4283 BsaBI GATnn'nnATC Cuts at: 6 1797 3834 6 Size: 1791 2037 455 Fragments arranged by size: 2037 1791 455 BsaHI Gr'CG_yC Cuts at: 1714 1962 2664 4160 1714 Size: 248 702 1496 1837 Fragments arranged by size: 1837 1496 702 248 BsaWI w'CCGG_w Cuts at: 1472 1993 2671 2945 3092 3503 1472 Size: 521 678 274 147 411 2252 Fragments arranged by size: 2252 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 482 512 889 919 1081 1111 482 Size: 30 377 30 162 30 3654 Fragments arranged by size: 3654 377 162 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 286 367 709 853 874 1195 1216 286 Size: 81 342 144 21 321 21 3353 Fragments arranged by size: 3353 342 321 144 81 21 21 BseYI C'CCAG_C Cuts at: 1708 2466 3043 3539 1708 Size: 758 577 496 2452 Fragments arranged by size: 2452 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1825 1871 3079 104 Size: 1721 46 1208 1308 Fragments arranged by size: 1721 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 866 1297 2075 2265 3057 866 Size: 431 778 190 792 2092 Fragments arranged by size: 2092 792 778 431 190 Bsp1286I G_dGCh'C Cuts at: 116 866 1297 1908 2001 2075 2265 2327 3057 Size: 750 431 611 93 74 190 62 730 Cuts at: 3057 116 Size: 1342 Fragments arranged by size: 1342 750 730 611 431 190 93 74 62 BspEI T'CCGG_A Cuts at: 3503 3503 Size: 4283 BspHI T'CATG_A Cuts at: 3459 3459 Size: 4283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1196 1333 1547 1849 2230 1196 Size: 137 214 302 381 3249 Fragments arranged by size: 3249 381 302 214 137 BsrBI CCG'CTC Cuts at: 50 2575 2629 4113 4278 50 Size: 2525 54 1484 165 55 Fragments arranged by size: 2525 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2195 2195 Size: 4283 BsrFI r'CCGG_y Cuts at: 2281 2462 2671 2281 Size: 181 209 3893 Fragments arranged by size: 3893 209 181 BssSI C'ACGA_G Cuts at: 2554 2912 2554 Size: 358 3925 BstAPI GCAn_nnn'nTGC Cuts at: 1660 1660 Size: 4283 BstBI TT'CG_AA Cuts at: 2644 2644 Size: 4283 BstYI r'GATC_y Cuts at: 1 1032 1308 1775 1798 2133 2379 3391 3477 Size: 1031 276 467 23 335 246 1012 86 Cuts at: 3477 3489 3835 1 Size: 12 346 449 Fragments arranged by size: 1031 1012 467 449 346 335 276 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 880 880 Size: 4283 BtgI C'CryG_G Cuts at: 107 107 Size: 4283 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2233 2414 2577 2233 Size: 181 163 3939 Fragments arranged by size: 3939 181 163 BtsI GCAGTG_nn' Cuts at: 142 1526 142 Size: 1384 2899 DraI TTT'AAA Cuts at: 1282 1282 Size: 4283 DraIII CAC_nnn'GTG Cuts at: 945 945 Size: 4283 DrdI GACnn_nn'nnGTC Cuts at: 174 1989 2847 174 Size: 1815 858 1610 Fragments arranged by size: 1815 1610 858 EaeI y'GGCC_r Cuts at: 440 1868 2042 2433 2460 3805 440 Size: 1428 174 391 27 1345 918 Fragments arranged by size: 1428 1345 918 391 174 27 EagI C'GGCC_G Cuts at: 1868 1868 Size: 4283 EarI CTCTTCn'nnn_ Cuts at: 794 830 2306 2516 794 Size: 36 1476 210 2561 Fragments arranged by size: 2561 1476 210 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2491 2801 2947 4093 2491 Size: 310 146 1146 2681 Fragments arranged by size: 2681 1146 310 146 EcoICRI GAG'CTC Cuts at: 1295 1295 Size: 4283 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 385 482 781 910 1228 1240 1252 2108 2540 Size: 97 299 129 318 12 12 856 432 Cuts at: 2540 3287 3555 385 Size: 747 268 1113 Fragments arranged by size: 1113 856 747 432 318 299 268 129 97 12 12 EcoO109I rG'GnC_Cy Cuts at: 112 564 1185 1422 112 Size: 452 621 237 2973 Fragments arranged by size: 2973 621 452 237 EcoRI G'AATT_C Cuts at: 1287 1287 Size: 4283 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2302 2302 Size: 4283 FspI TGC'GCA Cuts at: 2064 2064 Size: 4283 HaeII r_GCGC'y Cuts at: 1717 1965 2987 3966 1717 Size: 248 1022 979 2034 Fragments arranged by size: 2034 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 313 345 482 514 2125 2155 2157 2187 313 Size: 32 137 32 1611 30 2 30 2409 Fragments arranged by size: 2409 1611 137 32 32 30 30 2 HincII GTy'rAC Cuts at: 849 1322 849 Size: 473 3810 KasI G'GCGC_C Cuts at: 1713 1961 1713 Size: 248 4035 KpnI G_GTAC'C Cuts at: 1303 1303 Size: 4283 MluI A'CGCG_T Cuts at: 3716 3716 Size: 4283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2954 3138 2954 Size: 184 4099 MscI TGG'CCA Cuts at: 442 2044 442 Size: 1602 2681 MslI CAynn'nnrTG Cuts at: 535 2272 2399 535 Size: 1737 127 2419 Fragments arranged by size: 2419 1737 127 MspA1I CmG'CkG Cuts at: 436 691 1394 1708 2068 3081 3326 436 Size: 255 703 314 360 1013 245 1393 Fragments arranged by size: 1393 1013 703 360 314 255 245 NaeI GCC'GGC Cuts at: 2464 2464 Size: 4283 NarI GG'CG_CC Cuts at: 1714 1962 1714 Size: 248 4035 NcoI C'CATG_G Cuts at: 107 107 Size: 4283 NgoMIV G'CCGG_C Cuts at: 2462 2462 Size: 4283 NspI r_CATG'y Cuts at: 477 1336 1836 2743 477 Size: 859 500 907 2017 Fragments arranged by size: 2017 907 859 500 PciI A'CATG_T Cuts at: 2739 2739 Size: 4283 PflMI CCAn_nnn'nTGG Cuts at: 945 945 Size: 4283 PfoI T'CCnGG_A Cuts at: 362 1229 362 Size: 867 3416 PmeI GTTT'AAAC Cuts at: 1282 1282 Size: 4283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3422 3454 3422 Size: 32 4251 PpuMI rG'GwC_Cy Cuts at: 564 1185 564 Size: 621 3662 PspOMI G'GGCC_C Cuts at: 112 112 Size: 4283 PstI C_TGCA'G Cuts at: 602 1330 2015 602 Size: 728 685 2870 Fragments arranged by size: 2870 728 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4283 PvuII CAG'CTG Cuts at: 436 691 1708 2068 436 Size: 255 1017 360 2651 Fragments arranged by size: 2651 1017 360 255 RsrII CG'GwC_CG Cuts at: 2478 2478 Size: 4283 SacI G_AGCT'C Cuts at: 1297 1297 Size: 4283 SalI G'TCGA_C Cuts at: 1320 1320 Size: 4283 SapI GCTCTTCn'nnn_ Cuts at: 794 2306 2516 794 Size: 1512 210 2561 Fragments arranged by size: 2561 1512 210 SbfI CC_TGCA'GG Cuts at: 1330 1330 Size: 4283 ScaI AGT'ACT Cuts at: 3800 3800 Size: 4283 SfcI C'TryA_G Cuts at: 33 598 1326 2011 3004 3195 33 Size: 565 728 685 993 191 1121 Fragments arranged by size: 1121 993 728 685 565 191 SfoI GGC'GCC Cuts at: 1715 1963 1715 Size: 248 4035 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4283 SmaI CCC'GGG Cuts at: 1305 1305 Size: 4283 SmlI C'TyrA_G Cuts at: 997 1438 2353 2845 3107 997 Size: 441 915 492 262 2173 Fragments arranged by size: 2173 915 492 441 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4283 SphI G_CATG'C Cuts at: 1336 1836 1336 Size: 500 3783 SspI AAT'ATT Cuts at: 615 615 Size: 4283 StuI AGG'CCT Cuts at: 328 455 790 328 Size: 127 335 3821 Fragments arranged by size: 3821 335 127 StyI C'CwwG_G Cuts at: 107 1417 1771 107 Size: 1310 354 2619 Fragments arranged by size: 2619 1310 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 186 1866 1999 2458 2667 186 Size: 1680 133 459 209 1802 Fragments arranged by size: 1802 1680 459 209 133 TatI w'GTAC_w Cuts at: 3798 3798 Size: 4283 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3800 3832 3800 Size: 32 4251 Tth111I GACn'n_nGTC Cuts at: 2080 2080 Size: 4283 XbaI T'CTAG_A Cuts at: 1314 1314 Size: 4283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1778 1778 Size: 4283 XmaI C'CCGG_G Cuts at: 1303 1303 Size: 4283 XmnI GAAnn'nnTTC Cuts at: 268 268 Size: 4283 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI