Restriction map for pF1KB6187
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspHI       AspI        
AvrII       BalI        BamHI       BbeI        BbrPI       
BcgI        BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       Bsp1407I    
BspEI       BspHI       BsrGI       BssHII      Bst1107I    
BstBI       BstYI       BstZ17I     Bsu36I      DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        Hin1I       HindIII     HpaI        
KasI        KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1457  GGTACC               G^GTACC
AccII        1738  CGCG                 CG^CG
AcsI          910  AAATTC               [GA]^AATT[CT]
AflIII        109  ACATGT               A^C[GA][CT]GT
AlwNI         122  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          910  AAATTC               [GA]^AATT[CT]
Asp718I      1457  GGTACC               G^GTACC
AvaI          647  CCCGAG               C^[CT]CG[GA]G
BanII         579  GGGCTC               G[GA]GC[CT]^C
BciVI        1011  GGATAC               GTATCC(6/5)
BglI         1057  GCCATGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1052  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1052  GCTCAGC              GC^T[ACGT]AGC
BsaWI        1008  ACCGGA               [AT]^CCGG[AT]
BseRI        1770  CTCCTC               GAGGAG(10/8)
BsgI         1931  CTGCAC               GTGCAG(16/14)
BsmI          393  GAATGC               GAATGC(1/-1)
BsoBI         647  CCCGAG               C^[CT]CG[GA]G
BspDI        1861  ATCGAT               AT^CGAT
BspLU11I      109  ACATGT               A^CATGT
BsrBI        1500  CCGCTC               CCGCTC(-3/-3)
BsrDI         133  CATTGC               GCAATG(2/0)
BssSI        1855  CACGAG               CACGAG(-5/-1)
BstAPI       1631  GCAGGCTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII         65  GGTCACC              G^GT[ACGT]ACC
BstPI          65  GGTCACC              G^GT[ACGT]ACC
BstUI        1738  CGCG                 CG^CG
BstXI         330  CCAGCCTATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1052  GCTCAGC              GC^T[ACGT]AGC
ClaI         1861  ATCGAT               AT^CGAT
CpoI         1718  CGGACCG              CG^G[AT]CCG
DrdI         1410  GACTCACTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1254  CCATGG               C^C[GA][CT]GG
EaeI         1758  TGGCCG               [CT]^GGCC[GA]
EarI          921  GAAGAG               CTCTTC(1/4)
EcoNI        1565  CCTCCCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I        65  GGTCACC              G^GT[ACGT]ACC
HaeII         123  AGCGCC               [GA]GCGC^[CT]
KpnI         1457  GGTACC               GGTAC^C
Ksp632I       921  GAAGAG               CTCTTC(1/4)
MaeII        1334  ACGT                 A^CGT
MseI         1654  TTAA                 T^TAA
MvnI         1738  CGCG                 CG^CG
NcoI         1254  CCATGG               C^CATGG
NspI          109  ACATGT               [GA]CATG^[CT]
PflMI         373  CCACCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          694  CTGCAG               CTGCA^G
RsrII        1718  CGGACCG              CG^G[AT]CCG
SexAI        1464  ACCAGGT              A^CC[AT]GGT
StuI         1196  AGGCCT               AGG^CCT
Tru9I        1654  TTAA                 T^TAA
Van91I        373  CCACCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          785  CCAAAACTGTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI         1816  GATCC                GGATC(4/5)
             1660  GGATC               
AviII        1628  TGCGCA               TGC^GCA
             1886  TGCGCA              
BbvCI        1619  CCTCAGC              CCTCAGC(-5/-2)
             1819  CCTCAGC             
BfaI           73  CTAG                 C^TAG
              517  CTAG                
BmyI          254  GTGCCC               G[AGT]GC[ACT]^C
              579  GGGCTC              
BsmAI         650  GAGAC                GTCTC(1/5)
              686  GAGAC               
Bsp1286I      254  GTGCCC               G[AGT]GC[ACT]^C
              579  GGGCTC              
BspMI         692  ACCTGC               ACCTGC(4/8)
              978  ACCTGC              
BsrFI        1577  ACCGGC               [GA]^CCGG[CT]
             1958  ACCGGC              
BsrI          629  ACTGG                ACTGG(1/-1)
              682  ACTGG               
Cfr10I       1577  ACCGGC               [GA]^CCGG[CT]
             1958  ACCGGC              
EcoT14I      1254  CCATGG               C^C[AT][AT]GG
             1505  CCTTGG              
FspI         1628  TGCGCA               TGC^GCA
             1886  TGCGCA              
HgaI          426  GACGC                GACGC(5/10)
              397  GCGTC               
HincII        313  GTTGAC               GT[CT]^[GA]AC
             1318  GTCAAC              
HindII        313  GTTGAC               GT[CT]^[GA]AC
             1318  GTCAAC              
MaeI           73  CTAG                 C^TAG
              517  CTAG                
MslI         1255  CATGGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1896  CATGATGATG          
SmlI         1243  CTCAAG               C^T[CT][GA]AG
             1936  CTCAAG              
StyI         1254  CCATGG               C^C[AT][AT]GG
             1505  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanI        
BbsI        BbvI        BcnI        BpmI        BpuAI       
BsaJI       BsiYI       BslI        BsmFI       BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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