Restriction map for pF1KB6187
 (Linear) (Six-base) MAPSORT of: KIBB6187.seq  Check: 4336  from: 1  to: 1980



KIBB6187.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     987    1980 
   Size:        987     993
 
Acc65I G'GTAC_C

Cuts at:      0    1457    1980 
   Size:       1457     523
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1218    1939    1980 
   Size:       1218     721      41

  Fragments arranged by size: 

                1218     721      41
 
AflIII A'CryG_T

Cuts at:      0     109    1980 
   Size:        109    1871
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1891    1980 
   Size:       1891      89
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     202     234    1980 
   Size:        202      32    1746

  Fragments arranged by size: 

                1746     202      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     127    1980 
   Size:        127    1853
 
ApoI r'AATT_y

Cuts at:      0     910    1980 
   Size:        910    1070
 
AvaI C'yCGr_G

Cuts at:      0     647    1980 
   Size:        647    1333
 
BanI G'GyrC_C

Cuts at:      0     253     632     706    1457    1574    1980 
   Size:        253     379      74     751     117     406

  Fragments arranged by size: 

                 751     406     379     253     117      74
 
BanII G_rGCy'C

Cuts at:      0     583    1980 
   Size:        583    1397
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     392     430    1224    1980 
   Size:        392      38     794     756

  Fragments arranged by size: 

                 794     756     392      38
 
BbvCI CC'TCA_GC

Cuts at:      0    1620    1820    1980 
   Size:       1620     200     160

  Fragments arranged by size: 

                1620     200     160
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1005    1980 
   Size:       1005     975
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1063    1980 
   Size:       1063     917
 
BlpI GC'TnA_GC

Cuts at:      0    1053    1980 
   Size:       1053     927
 
Bme1580I G_kGCm'C

Cuts at:      0     258    1980 
   Size:        258    1722
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     971    1287    1980 
   Size:        971     316     693

  Fragments arranged by size: 

                 971     693     316
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704    1369    1626    1750    1980 
   Size:        704     665     257     124     230

  Fragments arranged by size: 

                 704     665     257     230     124
 
Bpu10I CC'TnA_GC

Cuts at:      0    1620    1820    1980 
   Size:       1620     200     160

  Fragments arranged by size: 

                1620     200     160
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1228    1921    1980 
   Size:       1228     693      59

  Fragments arranged by size: 

                1228     693      59
 
BsaWI w'CCGG_w

Cuts at:      0    1008    1980 
   Size:       1008     972
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     984    1014    1980 
   Size:        984      30     966

  Fragments arranged by size: 

                 984     966      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1761    1980 
   Size:       1761     219
 
BseYI C'CCAG_C

Cuts at:      0     120     555     766    1048    1311    1923    1980 
   Size:        120     435     211     282     263     612      57

  Fragments arranged by size: 

                 612     435     282     263     211     120      57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1916    1980 
   Size:       1916      64
 
BsmI GAATG_Cn'

Cuts at:      0     399    1980 
   Size:        399    1581
 
Bsp1286I G_dGCh'C

Cuts at:      0     258     583    1980 
   Size:        258     325    1397

  Fragments arranged by size: 

                1397     325     258
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     701     987    1980 
   Size:        701     286     993

  Fragments arranged by size: 

                 993     701     286
 
BsrBI CCG'CTC

Cuts at:      0    1502    1980 
   Size:       1502     478
 
BsrDI GCAATG_nn'

Cuts at:      0     132    1980 
   Size:        132    1848
 
BsrFI r'CCGG_y

Cuts at:      0    1577    1958    1980 
   Size:       1577     381      22

  Fragments arranged by size: 

                1577     381      22
 
BssSI C'ACGA_G

Cuts at:      0    1855    1980 
   Size:       1855     125
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1637    1980 
   Size:       1637     343
 
BstEII G'GTnAC_C

Cuts at:      0      65    1980 
   Size:         65    1915
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     337    1980 
   Size:        337    1643
 
BtgI C'CryG_G

Cuts at:      0    1254    1980 
   Size:       1254     726
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     464    1754    1980 
   Size:        464    1290     226

  Fragments arranged by size: 

                1290     464     226
 
BtsI GCAGTG_nn'

Cuts at:      0    1424    1613    1896    1980 
   Size:       1424     189     283      84

  Fragments arranged by size: 

                1424     283     189      84
 
ClaI AT'CG_AT

Cuts at:      0    1862    1980 
   Size:       1862     118
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     210     222     245     257    1536    1571    1980 
   Size:        210      12      23      12    1279      35     409

  Fragments arranged by size: 

                1279     409     210      35      23      12      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1416    1980 
   Size:       1416     564
 
EaeI y'GGCC_r

Cuts at:      0    1758    1980 
   Size:       1758     222
 
EarI CTCTTCn'nnn_

Cuts at:      0     916    1980 
   Size:        916    1064
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704    1218    1369    1626    1750    1939    1980 
   Size:        704     514     151     257     124     189      41

  Fragments arranged by size: 

                 704     514     257     189     151     124      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1569    1980 
   Size:       1569     411
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42     201     617     928    1065    1108    1764    1872 
   Size:         42     159     416     311     137      43     656     108

Cuts at:   1872    1927    1980 
   Size:         55      53

  Fragments arranged by size: 

                 656     416     311     159     137     108      55      53
                  43      42
 
FspI TGC'GCA

Cuts at:      0    1630    1888    1980 
   Size:       1630     258      92

  Fragments arranged by size: 

                1630     258      92
 
HaeII r_GCGC'y

Cuts at:      0     127    1980 
   Size:        127    1853
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     463     495    1522    1554    1669    1701    1756    1788 
   Size:        463      32    1027      32     115      32      55      32

Cuts at:   1788    1980 
   Size:        192

  Fragments arranged by size: 

                1027     463     192     115      55      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     315    1320    1980 
   Size:        315    1005     660

  Fragments arranged by size: 

                1005     660     315
 
KpnI G_GTAC'C

Cuts at:      0    1461    1980 
   Size:       1461     519
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     222    1697    1980 
   Size:        222    1475     283

  Fragments arranged by size: 

                1475     283     222
 
MslI CAynn'nnrTG

Cuts at:      0    1259    1900    1980 
   Size:       1259     641      80

  Fragments arranged by size: 

                1259     641      80
 
MspA1I CmG'CkG

Cuts at:      0     322     898    1624    1980 
   Size:        322     576     726     356

  Fragments arranged by size: 

                 726     576     356     322
 
NcoI C'CATG_G

Cuts at:      0    1254    1980 
   Size:       1254     726
 
NspI r_CATG'y

Cuts at:      0     113    1980 
   Size:        113    1867
 
PasI CC'CwG_GG

Cuts at:      0    1711    1980 
   Size:       1711     269
 
PciI A'CATG_T

Cuts at:      0     109    1980 
   Size:        109    1871
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     379    1980 
   Size:        379    1601
 
PfoI T'CCnGG_A

Cuts at:      0    1346    1805    1980 
   Size:       1346     459     175

  Fragments arranged by size: 

                1346     459     175
 
PpuMI rG'GwC_Cy

Cuts at:      0      42     201     928    1764    1980 
   Size:         42     159     727     836     216

  Fragments arranged by size: 

                 836     727     216     159      42
 
PstI C_TGCA'G

Cuts at:      0     698    1980 
   Size:        698    1282
 
PvuII CAG'CTG

Cuts at:      0     322     898    1624    1980 
   Size:        322     576     726     356

  Fragments arranged by size: 

                 726     576     356     322
 
RsrII CG'GwC_CG

Cuts at:      0    1719    1980 
   Size:       1719     261
 
SanDI GG'GwC_CC

Cuts at:      0    1764    1980 
   Size:       1764     216
 
SexAI A'CCwGG_T

Cuts at:      0    1464    1980 
   Size:       1464     516
 
SfcI C'TryA_G

Cuts at:      0      12     367     694    1980 
   Size:         12     355     327    1286

  Fragments arranged by size: 

                1286     355     327      12
 
SmlI C'TyrA_G

Cuts at:      0    1243    1936    1980 
   Size:       1243     693      44

  Fragments arranged by size: 

                1243     693      44
 
StuI AGG'CCT

Cuts at:      0    1198    1980 
   Size:       1198     782
 
StyI C'CwwG_G

Cuts at:      0    1254    1505    1980 
   Size:       1254     251     475

  Fragments arranged by size: 

                1254     475     251
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     596     867    1736    1980 
   Size:        596     271     869     244

  Fragments arranged by size: 

                 869     596     271     244
 
TatI w'GTAC_w

Cuts at:      0    1436    1907    1980 
   Size:       1436     471      73

  Fragments arranged by size: 

                1436     471      73
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     792    1980 
   Size:        792    1188
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AlfI     AloI    AlwNI     ApoI
     AvaI     BanI    BanII     BbsI    BbvCI    BciVI     BglI     BlpI
 Bme1580I     BplI     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI    BseRI
    BseYI     BsgI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FspI
    HaeII    Hin4I   HincII     KpnI     MmeI     MslI   MspA1I     NcoI
     NspI     PasI     PciI    PflMI     PfoI    PpuMI     PstI    PvuII
    RsrII    SanDI    SexAI     SfcI     SmlI     StuI     StyI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AleI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BcgI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI
     BsaI    BsaAI    BsaBI    BsaHI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrGI   BssHII    BstBI    BstYI  BstZ17I   Bsu36I
     DraI   DraIII     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI    FspAI  HindIII     HpaI     KasI     MfeI     MluI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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