Restriction map for pF1KB7985
 (Linear) (Six-base) MAPSORT of: KIBB7985.seq  Check: 8058  from: 1  to: 1293



KIBB7985.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AclI AA'CG_TT

Cuts at:      0     836    1293 
   Size:        836     457
 
AflIII A'CryG_T

Cuts at:      0     716     899    1293 
   Size:        716     183     394

  Fragments arranged by size: 

                 716     394     183
 
AleI CACnn'nnGTG

Cuts at:      0     705    1293 
   Size:        705     588
 
AlwNI CAG_nnn'CTG

Cuts at:      0     934    1293 
   Size:        934     359
 
ApoI r'AATT_y

Cuts at:      0     913     937    1293 
   Size:        913      24     356

  Fragments arranged by size: 

                 913     356      24
 
AvaI C'yCGr_G

Cuts at:      0     474     703    1293 
   Size:        474     229     590

  Fragments arranged by size: 

                 590     474     229
 
BamHI G'GATC_C

Cuts at:      0     345    1293 
   Size:        345     948
 
BanI G'GyrC_C

Cuts at:      0      40    1293 
   Size:         40    1253
 
BanII G_rGCy'C

Cuts at:      0     302    1091    1293 
   Size:        302     789     202

  Fragments arranged by size: 

                 789     302     202
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     476     603    1293 
   Size:        476     127     690

  Fragments arranged by size: 

                 690     476     127
 
BbvCI CC'TCA_GC

Cuts at:      0     643    1222    1293 
   Size:        643     579      71

  Fragments arranged by size: 

                 643     579      71
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     901     935    1293 
   Size:        901      34     358

  Fragments arranged by size: 

                 901     358      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1201    1293 
   Size:       1201      92
 
BglII A'GATC_T

Cuts at:      0     769    1293 
   Size:        769     524
 
BlpI GC'TnA_GC

Cuts at:      0     400    1293 
   Size:        400     893
 
Bme1580I G_kGCm'C

Cuts at:      0      79    1293 
   Size:         79    1214
 
BmgBI CAC'GTC

Cuts at:      0     207    1293 
   Size:        207    1086
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     177    1293 
   Size:        177    1116
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1105    1293 
   Size:       1105     188
 
Bpu10I CC'TnA_GC

Cuts at:      0     405     643    1040    1222    1293 
   Size:        405     238     397     182      71

  Fragments arranged by size: 

                 405     397     238     182      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1183    1293 
   Size:       1183     110
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     410    1293 
   Size:        410     883
 
BsaAI yAC'GTr

Cuts at:      0     900    1293 
   Size:        900     393
 
BsaWI w'CCGG_w

Cuts at:      0     740    1293 
   Size:        740     553
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     857     887    1111    1141    1293 
   Size:        857      30     224      30     152

  Fragments arranged by size: 

                 857     224     152      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     466     631    1291    1293 
   Size:        466     165     660       2

  Fragments arranged by size: 

                 660     466     165       2
 
BseYI C'CCAG_C

Cuts at:      0      68      72     242     726    1293 
   Size:         68       4     170     484     567

  Fragments arranged by size: 

                 567     484     170      68       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     289     367     703    1293 
   Size:        220      69      78     336     590

  Fragments arranged by size: 

                 590     336     220      78      69
 
BsiHKAI G_wGCw'C

Cuts at:      0     740    1293 
   Size:        740     553
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     555     813    1293 
   Size:        555     258     480

  Fragments arranged by size: 

                 555     480     258
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     302     740    1091    1293 
   Size:         79     223     438     351     202

  Fragments arranged by size: 

                 438     351     223     202      79
 
BspEI T'CCGG_A

Cuts at:      0     740    1293 
   Size:        740     553
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1040    1293 
   Size:       1040     253
 
BsrDI GCAATG_nn'

Cuts at:      0     549    1293 
   Size:        549     744
 
BssSI C'ACGA_G

Cuts at:      0      99     370    1293 
   Size:         99     271     923

  Fragments arranged by size: 

                 923     271      99
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     295    1293 
   Size:        295     998
 
BstYI r'GATC_y

Cuts at:      0     192     345     769    1293 
   Size:        192     153     424     524

  Fragments arranged by size: 

                 524     424     192     153
 
BtgI C'CryG_G

Cuts at:      0      23    1131    1148    1160    1205    1293 
   Size:         23    1108      17      12      45      88

  Fragments arranged by size: 

                1108      88      45      23      17      12
 
BtsI GCAGTG_nn'

Cuts at:      0    1272    1293 
   Size:       1272      21
 
EaeI y'GGCC_r

Cuts at:      0     273    1293 
   Size:        273    1020
 
EarI CTCTTCn'nnn_

Cuts at:      0     586    1293 
   Size:        586     707
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     543     691     746    1293 
   Size:        543     148      55     547

  Fragments arranged by size: 

                 547     543     148      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1105    1293 
   Size:       1105     188
 
EcoO109I rG'GnC_Cy

Cuts at:      0     375     456     514    1194    1293 
   Size:        375      81      58     680      99

  Fragments arranged by size: 

                 680     375      99      81      58
 
HaeII r_GCGC'y

Cuts at:      0     107    1293 
   Size:        107    1186
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     184     216     857     889    1293 
   Size:        184      32     641      32     404

  Fragments arranged by size: 

                 641     404     184      32      32
 
HincII GTy'rAC

Cuts at:      0     210    1293 
   Size:        210    1083
 
MluI A'CGCG_T

Cuts at:      0     716    1293 
   Size:        716     577
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     560    1293 
   Size:        560     733
 
MslI CAynn'nnrTG

Cuts at:      0     705    1293 
   Size:        705     588
 
MspA1I CmG'CkG

Cuts at:      0      72     114     333     687    1150    1162    1293 
   Size:         72      42     219     354     463      12     131

  Fragments arranged by size: 

                 463     354     219     131      72      42      12
 
NcoI C'CATG_G

Cuts at:      0      23    1205    1293 
   Size:         23    1182      88

  Fragments arranged by size: 

                1182      88      23
 
NspI r_CATG'y

Cuts at:      0     466     993    1293 
   Size:        466     527     300

  Fragments arranged by size: 

                 527     466     300
 
PasI CC'CwG_GG

Cuts at:      0    1056    1293 
   Size:       1056     237
 
PfoI T'CCnGG_A

Cuts at:      0    1082    1293 
   Size:       1082     211
 
PpuMI rG'GwC_Cy

Cuts at:      0     375     456    1293 
   Size:        375      81     837

  Fragments arranged by size: 

                 837     375      81
 
PstI C_TGCA'G

Cuts at:      0     816    1178    1293 
   Size:        816     362     115

  Fragments arranged by size: 

                 816     362     115
 
PvuII CAG'CTG

Cuts at:      0      72     333    1293 
   Size:         72     261     960

  Fragments arranged by size: 

                 960     261      72
 
SacII CC_GC'GG

Cuts at:      0    1151    1163    1293 
   Size:       1151      12     130

  Fragments arranged by size: 

                1151     130      12
 
SbfI CC_TGCA'GG

Cuts at:      0     816    1293 
   Size:        816     477
 
SfcI C'TryA_G

Cuts at:      0      80     219     360     812    1174    1293 
   Size:         80     139     141     452     362     119

  Fragments arranged by size: 

                 452     362     141     139     119      80
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1201    1293 
   Size:       1201      92
 
SmaI CCC'GGG

Cuts at:      0     476    1293 
   Size:        476     817
 
SmlI C'TyrA_G

Cuts at:      0    1198    1293 
   Size:       1198      95
 
SpeI A'CTAG_T

Cuts at:      0     855    1293 
   Size:        855     438
 
StyI C'CwwG_G

Cuts at:      0      23    1205    1293 
   Size:         23    1182      88

  Fragments arranged by size: 

                1182      88      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     275    1061    1293 
   Size:        275     786     232

  Fragments arranged by size: 

                 786     275     232
 
TatI w'GTAC_w

Cuts at:      0     659    1293 
   Size:        659     634
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1080    1293 
   Size:       1080     213
 
XmaI C'CCGG_G

Cuts at:      0     474    1293 
   Size:        474     819
 

 Enzymes that do cut:

     AclI   AflIII     AleI    AlwNI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspMI    BsrDI    BssSI    BstXI    BstYI     BtgI     BtsI     EaeI
     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     MluI
     MmeI     MslI   MspA1I     NcoI     NspI     PasI     PfoI    PpuMI
     PstI    PvuII    SacII     SbfI     SfcI     SfiI     SmaI     SmlI
     SpeI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BciVI     BclI    BfrBI     BmtI     BplI
    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     ScaI    SexAI     SfoI
     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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