Restriction map for pF1KB7865
 (Linear) (Six-base) MAPSORT of: KIBB7865.seq  Check: 8490  from: 1  to: 1671



KIBB7865.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AccI GT'mk_AC

Cuts at:      0     887    1671 
   Size:        887     784
 
AflIII A'CryG_T

Cuts at:      0    1532    1634    1671 
   Size:       1532     102      37

  Fragments arranged by size: 

                1532     102      37
 
AgeI A'CCGG_T

Cuts at:      0     701    1671 
   Size:        701     970
 
AlwNI CAG_nnn'CTG

Cuts at:      0     606    1431    1671 
   Size:        606     825     240

  Fragments arranged by size: 

                 825     606     240
 
ApaI G_GGCC'C

Cuts at:      0     719    1671 
   Size:        719     952
 
BanI G'GyrC_C

Cuts at:      0     469    1671 
   Size:        469    1202
 
BanII G_rGCy'C

Cuts at:      0      55     167     238     677     719     862    1471    1671 
   Size:         55     112      71     439      42     143     609     200

  Fragments arranged by size: 

                 609     439     200     143     112      71      55      42
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     174     487    1631    1671 
   Size:        174     313    1144      40

  Fragments arranged by size: 

                1144     313     174      40
 
BclI T'GATC_A

Cuts at:      0     371    1671 
   Size:        371    1300
 
BlpI GC'TnA_GC

Cuts at:      0      49    1671 
   Size:         49    1622
 
Bme1580I G_kGCm'C

Cuts at:      0     472     719    1671 
   Size:        472     247     952

  Fragments arranged by size: 

                 952     472     247
 
BmgBI CAC'GTC

Cuts at:      0      17    1671 
   Size:         17    1654
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1228    1554    1671 
   Size:       1228     326     117

  Fragments arranged by size: 

                1228     326     117
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     553    1671 
   Size:        553    1118
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     148    1337    1671 
   Size:          7     141    1189     334

  Fragments arranged by size: 

                1189     334     141       7
 
Bpu10I CC'TnA_GC

Cuts at:      0      37    1671 
   Size:         37    1634
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259    1671 
   Size:        259    1412
 
BsaHI Gr'CG_yC

Cuts at:      0     947    1671 
   Size:        947     724
 
BsaWI w'CCGG_w

Cuts at:      0     332     701    1671 
   Size:        332     369     970

  Fragments arranged by size: 

                 970     369     332
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     220     250    1249    1279    1671 
   Size:        220      30     999      30     392

  Fragments arranged by size: 

                 999     392     220      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     316     931    1011    1016    1077    1174    1235 
   Size:        106     210     615      80       5      61      97      61

Cuts at:   1235    1671 
   Size:        436

  Fragments arranged by size: 

                 615     436     210     106      97      80      61      61
                   5
 
BseYI C'CCAG_C

Cuts at:      0     452     725     766    1015    1155    1280    1424    1487 
   Size:        452     273      41     249     140     125     144      63

Cuts at:   1487    1671 
   Size:        184

  Fragments arranged by size: 

                 452     273     249     184     144     140     125      63
                  41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1417    1671 
   Size:       1417     254
 
BsiEI CG_ry'CG

Cuts at:      0     753    1671 
   Size:        753     918
 
BsiHKAI G_wGCw'C

Cuts at:      0      55    1671 
   Size:         55    1616
 
BsmI GAATG_Cn'

Cuts at:      0     805     922    1671 
   Size:        805     117     749

  Fragments arranged by size: 

                 805     749     117
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     124    1671 
   Size:        124    1547
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     167     238     472     677     719     862    1471 
   Size:         55     112      71     234     205      42     143     609

Cuts at:   1471    1671 
   Size:        200

  Fragments arranged by size: 

                 609     234     205     200     143     112      71      55
                  42
 
BspHI T'CATG_A

Cuts at:      0    1301    1671 
   Size:       1301     370
 
BsrBI CCG'CTC

Cuts at:      0     499    1671 
   Size:        499    1172
 
BsrDI GCAATG_nn'

Cuts at:      0    1598    1671 
   Size:       1598      73
 
BsrFI r'CCGG_y

Cuts at:      0     177     427     701    1387    1671 
   Size:        177     250     274     686     284

  Fragments arranged by size: 

                 686     284     274     250     177
 
BsrGI T'GTAC_A

Cuts at:      0     503    1671 
   Size:        503    1168
 
BstEII G'GTnAC_C

Cuts at:      0      60    1122    1671 
   Size:         60    1062     549

  Fragments arranged by size: 

                1062     549      60
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     568     646    1671 
   Size:        568      78    1025

  Fragments arranged by size: 

                1025     568      78
 
BstYI r'GATC_y

Cuts at:      0     533    1671 
   Size:        533    1138
 
BtgI C'CryG_G

Cuts at:      0     639     849     941     993    1104    1118    1671 
   Size:        639     210      92      52     111      14     553

  Fragments arranged by size: 

                 639     553     210     111      92      52      14
 
DraIII CAC_nnn'GTG

Cuts at:      0    1363    1671 
   Size:       1363     308
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1131    1671 
   Size:       1131     540
 
EaeI y'GGCC_r

Cuts at:      0     201     938    1385    1671 
   Size:        201     737     447     286

  Fragments arranged by size: 

                 737     447     286     201
 
EarI CTCTTCn'nnn_

Cuts at:      0     770    1671 
   Size:        770     901
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     325     337    1671 
   Size:        325      12    1334

  Fragments arranged by size: 

                1334     325      12
 
EcoICRI GAG'CTC

Cuts at:      0      53    1671 
   Size:         53    1618
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     148    1337    1671 
   Size:          7     141    1189     334

  Fragments arranged by size: 

                1189     334     141       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     348     715    1671 
   Size:        348     367     956

  Fragments arranged by size: 

                 956     367     348
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     198     234    1671 
   Size:        198      36    1437

  Fragments arranged by size: 

                1437     198      36
 
HaeII r_GCGC'y

Cuts at:      0      44     292    1118    1671 
   Size:         44     248     826     553

  Fragments arranged by size: 

                 826     553     248      44
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     220     252     652     684    1671 
   Size:        220      32     400      32     987

  Fragments arranged by size: 

                 987     400     220      32      32
 
HincII GTy'rAC

Cuts at:      0     631    1671 
   Size:        631    1040
 
MfeI C'AATT_G

Cuts at:      0    1328    1671 
   Size:       1328     343
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     564     887     983    1671 
   Size:        564     323      96     688

  Fragments arranged by size: 

                 688     564     323      96
 
MscI TGG'CCA

Cuts at:      0     940    1671 
   Size:        940     731
 
MslI CAynn'nnrTG

Cuts at:      0     475    1633    1671 
   Size:        475    1158      38

  Fragments arranged by size: 

                1158     475      38
 
MspA1I CmG'CkG

Cuts at:      0     155     318     603    1431    1507    1518    1671 
   Size:        155     163     285     828      76      11     153

  Fragments arranged by size: 

                 828     285     163     155     153      76      11
 
NaeI GCC'GGC

Cuts at:      0    1389    1671 
   Size:       1389     282
 
NcoI C'CATG_G

Cuts at:      0     639     941    1104    1118    1671 
   Size:        639     302     163      14     553

  Fragments arranged by size: 

                 639     553     302     163      14
 
NgoMIV G'CCGG_C

Cuts at:      0    1387    1671 
   Size:       1387     284
 
NspI r_CATG'y

Cuts at:      0     387    1536    1638    1671 
   Size:        387    1149     102      33

  Fragments arranged by size: 

                1149     387     102      33
 
PciI A'CATG_T

Cuts at:      0    1532    1634    1671 
   Size:       1532     102      37

  Fragments arranged by size: 

                1532     102      37
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     855    1671 
   Size:        855     816
 
PpuMI rG'GwC_Cy

Cuts at:      0     348    1671 
   Size:        348    1323
 
PspOMI G'GGCC_C

Cuts at:      0     715    1671 
   Size:        715     956
 
PstI C_TGCA'G

Cuts at:      0     287    1409    1523    1671 
   Size:        287    1122     114     148

  Fragments arranged by size: 

                1122     287     148     114
 
PvuI CG_AT'CG

Cuts at:      0     753    1671 
   Size:        753     918
 
PvuII CAG'CTG

Cuts at:      0     603    1431    1507    1518    1671 
   Size:        603     828      76      11     153

  Fragments arranged by size: 

                 828     603     153      76      11
 
SacI G_AGCT'C

Cuts at:      0      55    1671 
   Size:         55    1616
 
SexAI A'CCwGG_T

Cuts at:      0     508    1671 
   Size:        508    1163
 
SfcI C'TryA_G

Cuts at:      0     283     951    1053    1405    1519    1671 
   Size:        283     668     102     352     114     152

  Fragments arranged by size: 

                 668     352     283     152     114     102
 
SmlI C'TyrA_G

Cuts at:      0     274    1671 
   Size:        274    1397
 
StuI AGG'CCT

Cuts at:      0      85     745     787    1671 
   Size:         85     660      42     884

  Fragments arranged by size: 

                 884     660      85      42
 
StyI C'CwwG_G

Cuts at:      0      65      86     209     352     639     941    1104    1118 
   Size:         65      21     123     143     287     302     163      14

Cuts at:   1118    1671 
   Size:        553

  Fragments arranged by size: 

                 553     302     287     163     143     123      65      21
                  14
 
TatI w'GTAC_w

Cuts at:      0     503    1621    1671 
   Size:        503    1118      50

  Fragments arranged by size: 

                1118     503      50
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     254     286     327     359    1249    1281    1349    1381 
   Size:        254      32      41      32     890      32      68      32

Cuts at:   1381    1671 
   Size:        290

  Fragments arranged by size: 

                 890     290     254      68      41      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      72    1671 
   Size:         72    1599
 

 Enzymes that do cut:

     AccI   AflIII     AgeI    AlwNI     ApaI     BanI    BanII     BbsI
     BclI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BstEII    BstXI    BstYI     BtgI   DraIII     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
   HincII     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI     PciI    PflMI    PpuMI   PspOMI     PstI     PvuI
    PvuII     SacI    SexAI     SfcI     SmlI     StuI     StyI     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BmtI     BsaI    BsaAI    BsaBI    BsiWI
    BspEI    BspMI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI     EagI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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