Restriction map for pF1KB5995
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AfaI        
AflII       AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BbeI        
BbrPI       BbsI        BcgI        BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsgI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BsrBI       
BsrFI       BsrGI       BssHII      BstBI       BstEII      
BstPI       CelII       Cfr10I      ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         238  CGCG                 CG^CG
AccIII        549  TCCGGA               T^CCGGA
AflIII        479  ACATGT               A^C[GA][CT]GT
AhdI          351  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         351  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1080  TGGCCA               TGG^CCA
BanI          319  GGTGCC               G^G[CT][GA]CC
BanII        1135  GAGCCC               G[GA]GC[CT]^C
BbvCI         613  CCTCAGC              CCTCAGC(-5/-2)
BsaWI         549  TCCGGA               [AT]^CCGG[AT]
BseAI         549  TCCGGA               T^CCGGA
BspEI         549  TCCGGA               T^CCGGA
BspHI        1602  TCATGA               T^CATGA
BspLU11I      479  ACATGT               A^CATGT
BspMI        1626  GCAGGT               ACCTGC(4/8)
BstAPI        208  GCACTTAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         238  CGCG                 CG^CG
DraI         1695  TTTAAA               TTT^AAA
DraIII        209  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1080  TGGCCA               [CT]^GGCC[GA]
Eam1105I      351  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         241  GAATTC               G^AATTC
EcoT22I       709  ATGCAT               ATGCA^T
HindIII       740  AAGCTT               A^AGCTT
HphI          337  TCACC                GGTGA(8/7)
MaeII          45  ACGT                 A^CGT
MluNI        1080  TGGCCA               TGG^CCA
MroI          549  TCCGGA               T^CCGGA
MscI         1080  TGGCCA               TGG^CCA
MslI          712  CATGTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          238  CGCG                 CG^CG
NsiI          709  ATGCAT               ATGCA^T
PaeR7I        953  CTCGAG               C^TCGAG
PshAI         863  GACCAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1643  CTGCAG               CTGCA^G
PvuII         782  CAGCTG               CAG^CTG
RcaI         1602  TCATGA               T^CATGA
SfcI         1643  CTGCAG               C^T[GA][CT]AG
SfiI          681  GGCCCCTCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1573  CCCGGG               CCC^GGG
SmlI          953  CTCGAG               C^T[CT][GA]AG
TfiI          672  GAATC                G^A[AT]TC
XhoI          953  CTCGAG               C^TCGAG
XmaCI        1573  CCCGGG               C^CCGGG
XmaI         1573  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          142  GTATAC               GT^[AC][GT]AC
             1538  GTATAC              
AcsI          241  GAATTC               [GA]^AATT[CT]
              566  AAATTC              
ApoI          241  GAATTC               [GA]^AATT[CT]
              566  AAATTC              
AspHI         254  GAGCAC               G[AT]GC[AT]^C
             1724  GAGCAC              
BciVI        1395  GGATAC               GTATCC(6/5)
             1685  GTATCC              
BcnI         1573  CCCGG                CC^[GC]GG
             1574  CCGGG               
BsaI           20  GAGACC               GGTCTC(1/5)
              349  GAGACC              
BseRI         728  CTCCTC               GAGGAG(10/8)
              824  GAGGAG              
BsiHKAI       254  GAGCAC               G[AT]GC[AT]^C
             1724  GAGCAC              
BssSI        1235  CACGAG               CACGAG(-5/-1)
             1325  CTCGTG              
Bst1107I      142  GTATAC               GTA^TAC
             1538  GTATAC              
BstZ17I       142  GTATAC               GTA^TAC
             1538  GTATAC              
CfoI          237  GCGC                 GCG^C
              722  GCGC                
EcoNI         637  CCTGGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              820  CCTGGAGGAGG         
FauI            4  GCGGG                CCCGC(4/6)
              921  GCGGG               
HapII         550  CCGG                 C^CGG
             1574  CCGG                
HhaI          237  GCGC                 GCG^C
              722  GCGC                
HinP1I        237  GCGC                 G^CGC
              722  GCGC                
HpaII         550  CCGG                 C^CGG
             1574  CCGG                
MseI         1463  TTAA                 T^TAA
             1696  TTAA                
MspI          550  CCGG                 C^CGG
             1574  CCGG                
NciI         1573  CCCGG                CC^[GC]GG
             1574  CCGGG               
NspI          479  ACATGT               [GA]CATG^[CT]
              711  GCATGT              
PleI          334  GAGTC                GAGTC(4/5)
              376  GAGTC               
TaqI          261  TCGA                 T^CGA
              954  TCGA                
Tru9I        1463  TTAA                 T^TAA
             1696  TTAA                
TthHB8I       261  TCGA                 T^CGA
              954  TCGA                
XcmI          693  CCAGTCTTTGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1139  CCAGACCTCTAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AocI        
AvaI        AvaII       BamHI       BbvI        BfaI        
BglI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MspA1I      MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PpuMI       SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XhoII       
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