(Circular) (Six-base) MAPSORT of: pF1KB6314.seq Check: 1605 from: 1 to: 3956 pF1KB6314 3956 bp With 182 enzymes: SgfI * April 24, 2009 13:05 .. AccI GT'mk_AC Cuts at: 457 692 740 994 457 Size: 235 48 254 3419 Fragments arranged by size: 3419 254 235 48 Acc65I G'GTAC_C Cuts at: 972 972 Size: 3956 AclI AA'CG_TT Cuts at: 3796 3796 Size: 3956 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 340 1781 2213 2960 3228 340 Size: 1441 432 747 268 1068 Fragments arranged by size: 1441 1068 747 432 268 AflIII A'CryG_T Cuts at: 1308 2412 3389 1308 Size: 1104 977 1875 Fragments arranged by size: 1875 1104 977 AgeI A'CCGG_T Cuts at: 2344 2344 Size: 3956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 736 736 Size: 3956 AlwNI CAG_nnn'CTG Cuts at: 771 1261 2828 771 Size: 490 1567 1899 Fragments arranged by size: 1899 1567 490 ApaI G_GGCC'C Cuts at: 180 828 180 Size: 648 3308 ApaLI G'TGCA_C Cuts at: 2726 2726 Size: 3956 ApoI r'AATT_y Cuts at: 960 3309 3565 960 Size: 2349 256 1351 Fragments arranged by size: 2349 1351 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3956 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3956 AvaI C'yCGr_G Cuts at: 976 976 Size: 3956 BamHI G'GATC_C Cuts at: 981 981 Size: 3956 BanI G'GyrC_C Cuts at: 972 1386 1634 1669 972 Size: 414 248 35 3259 Fragments arranged by size: 3259 414 248 35 BanII G_rGCy'C Cuts at: 180 828 899 970 2000 180 Size: 648 71 71 1030 2136 Fragments arranged by size: 2136 1030 648 71 71 BbeI G_GCGC'C Cuts at: 1390 1638 1390 Size: 248 3708 BbsI GAAGACnn'nnnn_ Cuts at: 602 602 Size: 3956 BbvCI CC'TCA_GC Cuts at: 535 535 Size: 3956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3922 BciVI GTATCCnnnnn_n' Cuts at: 770 1854 2615 3956 770 Size: 1084 761 1341 770 Fragments arranged by size: 1341 1084 770 761 BclI T'GATC_A Cuts at: 653 870 1476 653 Size: 217 606 3133 Fragments arranged by size: 3133 606 217 BglI GCCn_nnn'nGGC Cuts at: 222 222 Size: 3956 BglII A'GATC_T Cuts at: 140 1471 3508 140 Size: 1331 2037 588 Fragments arranged by size: 2037 1331 588 BlpI GC'TnA_GC Cuts at: 1068 1068 Size: 3956 Bme1580I G_kGCm'C Cuts at: 180 218 828 1581 1674 2730 180 Size: 38 610 753 93 1056 1406 Fragments arranged by size: 1406 1056 753 610 93 38 BmrI ACTGGGnnnn_n' Cuts at: 156 503 674 821 1168 1249 1584 3728 156 Size: 347 171 147 347 81 335 2144 384 Fragments arranged by size: 2144 384 347 347 335 171 147 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2323 2323 Size: 3956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 167 644 167 Size: 477 3479 Bpu10I CC'TnA_GC Cuts at: 535 727 535 Size: 192 3764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 555 1132 2011 2503 2801 555 Size: 577 879 492 298 1710 Fragments arranged by size: 1710 879 577 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3660 3660 Size: 3956 BsaAI yAC'GTr Cuts at: 1309 1309 Size: 3956 BsaBI GATnn'nnATC Cuts at: 6 1470 3507 6 Size: 1464 2037 455 Fragments arranged by size: 2037 1464 455 BsaHI Gr'CG_yC Cuts at: 1387 1635 2337 3833 1387 Size: 248 702 1496 1510 Fragments arranged by size: 1510 1496 702 248 BsaWI w'CCGG_w Cuts at: 1145 1666 2344 2618 2765 3176 1145 Size: 521 678 274 147 411 1925 Fragments arranged by size: 1925 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1381 2139 2716 3212 1381 Size: 758 577 496 2125 Fragments arranged by size: 2125 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 789 789 Size: 3956 BsiEI CG_ry'CG Cuts at: 104 1498 1544 2752 104 Size: 1394 46 1208 1308 Fragments arranged by size: 1394 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 899 970 1748 1938 2730 899 Size: 71 778 190 792 2125 Fragments arranged by size: 2125 792 778 190 71 Bsp1286I G_dGCh'C Cuts at: 180 218 828 899 970 1581 1674 1748 1938 Size: 38 610 71 71 611 93 74 190 Cuts at: 1938 2000 2730 180 Size: 62 730 1406 Fragments arranged by size: 1406 730 611 610 190 93 74 71 71 62 38 BspEI T'CCGG_A Cuts at: 3176 3176 Size: 3956 BspHI T'CATG_A Cuts at: 3132 3132 Size: 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 728 1006 1220 1522 1903 728 Size: 278 214 302 381 2781 Fragments arranged by size: 2781 381 302 278 214 BsrBI CCG'CTC Cuts at: 50 2248 2302 3786 3951 50 Size: 2198 54 1484 165 55 Fragments arranged by size: 2198 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1868 1868 Size: 3956 BsrFI r'CCGG_y Cuts at: 1954 2135 2344 1954 Size: 181 209 3566 Fragments arranged by size: 3566 209 181 BssSI C'ACGA_G Cuts at: 2227 2585 2227 Size: 358 3598 BstAPI GCAn_nnn'nTGC Cuts at: 1333 1333 Size: 3956 BstBI TT'CG_AA Cuts at: 2317 2317 Size: 3956 BstXI CCAn_nnnn'nTGG Cuts at: 585 585 Size: 3956 BstYI r'GATC_y Cuts at: 1 140 768 981 1448 1471 1806 2052 3064 Size: 139 628 213 467 23 335 246 1012 Cuts at: 3064 3150 3162 3508 1 Size: 86 12 346 449 Fragments arranged by size: 1012 628 467 449 346 335 246 213 139 86 23 12 BstZ17I GTA'TAC Cuts at: 693 693 Size: 3956 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1906 2087 2250 1906 Size: 181 163 3612 Fragments arranged by size: 3612 181 163 BtsI GCAGTG_nn' Cuts at: 165 292 854 1199 165 Size: 127 562 345 2922 Fragments arranged by size: 2922 562 345 127 DraI TTT'AAA Cuts at: 955 955 Size: 3956 DrdI GACnn_nn'nnGTC Cuts at: 1662 2520 1662 Size: 858 3098 EaeI y'GGCC_r Cuts at: 1541 1715 2106 2133 3478 1541 Size: 174 391 27 1345 2019 Fragments arranged by size: 2019 1345 391 174 27 EagI C'GGCC_G Cuts at: 1541 1541 Size: 3956 EarI CTCTTCn'nnn_ Cuts at: 319 424 1979 2189 319 Size: 105 1555 210 2086 Fragments arranged by size: 2086 1555 210 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 266 2164 2474 2620 3766 266 Size: 1898 310 146 1146 456 Fragments arranged by size: 1898 1146 456 310 146 EcoICRI GAG'CTC Cuts at: 897 968 897 Size: 71 3885 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 167 340 644 1781 2213 2960 3228 167 Size: 173 304 1137 432 747 268 895 Fragments arranged by size: 1137 895 747 432 304 268 173 EcoNI CCTnn'n_nnAGG Cuts at: 430 430 Size: 3956 EcoO109I rG'GnC_Cy Cuts at: 177 1095 177 Size: 918 3038 EcoRI G'AATT_C Cuts at: 960 960 Size: 3956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1975 1975 Size: 3956 FspI TGC'GCA Cuts at: 1737 1737 Size: 3956 HaeII r_GCGC'y Cuts at: 1390 1638 2660 3639 1390 Size: 248 1022 979 1707 Fragments arranged by size: 1707 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1798 1828 1830 1860 1798 Size: 30 2 30 3894 Fragments arranged by size: 3894 30 30 2 HincII GTy'rAC Cuts at: 885 995 885 Size: 110 3846 KasI G'GCGC_C Cuts at: 1386 1634 1386 Size: 248 3708 KpnI G_GTAC'C Cuts at: 976 976 Size: 3956 MluI A'CGCG_T Cuts at: 3389 3389 Size: 3956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 229 590 2627 2811 229 Size: 361 2037 184 1374 Fragments arranged by size: 2037 1374 361 184 MscI TGG'CCA Cuts at: 1717 1717 Size: 3956 MslI CAynn'nnrTG Cuts at: 878 1945 2072 878 Size: 1067 127 2762 Fragments arranged by size: 2762 1067 127 MspA1I CmG'CkG Cuts at: 309 1067 1381 1741 2754 2999 309 Size: 758 314 360 1013 245 1266 Fragments arranged by size: 1266 1013 758 360 314 245 NaeI GCC'GGC Cuts at: 2137 2137 Size: 3956 NarI GG'CG_CC Cuts at: 1387 1635 1387 Size: 248 3708 NgoMIV G'CCGG_C Cuts at: 2135 2135 Size: 3956 NspI r_CATG'y Cuts at: 1009 1509 2416 1009 Size: 500 907 2549 Fragments arranged by size: 2549 907 500 PasI CC'CwG_GG Cuts at: 173 173 Size: 3956 PciI A'CATG_T Cuts at: 2412 2412 Size: 3956 PflMI CCAn_nnn'nTGG Cuts at: 771 771 Size: 3956 PmeI GTTT'AAAC Cuts at: 955 955 Size: 3956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3095 3127 3095 Size: 32 3924 PspOMI G'GGCC_C Cuts at: 176 824 176 Size: 648 3308 PstI C_TGCA'G Cuts at: 1003 1688 1003 Size: 685 3271 PvuI CG_AT'CG Cuts at: 104 104 Size: 3956 PvuII CAG'CTG Cuts at: 1381 1741 1381 Size: 360 3596 RsrII CG'GwC_CG Cuts at: 2151 2151 Size: 3956 SacI G_AGCT'C Cuts at: 899 970 899 Size: 71 3885 SalI G'TCGA_C Cuts at: 993 993 Size: 3956 SapI GCTCTTCn'nnn_ Cuts at: 424 1979 2189 424 Size: 1555 210 2191 Fragments arranged by size: 2191 1555 210 SbfI CC_TGCA'GG Cuts at: 1003 1003 Size: 3956 ScaI AGT'ACT Cuts at: 3473 3473 Size: 3956 SfcI C'TryA_G Cuts at: 33 427 999 1684 2677 2868 33 Size: 394 572 685 993 191 1121 Fragments arranged by size: 1121 993 685 572 394 191 SfoI GGC'GCC Cuts at: 1388 1636 1388 Size: 248 3708 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3956 SmaI CCC'GGG Cuts at: 978 978 Size: 3956 SmlI C'TyrA_G Cuts at: 570 1111 2026 2518 2780 570 Size: 541 915 492 262 1746 Fragments arranged by size: 1746 915 541 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3956 SphI G_CATG'C Cuts at: 1009 1509 1009 Size: 500 3456 StuI AGG'CCT Cuts at: 225 347 225 Size: 122 3834 StyI C'CwwG_G Cuts at: 386 1090 1444 386 Size: 704 354 2898 Fragments arranged by size: 2898 704 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 138 1539 1672 2131 2340 138 Size: 1401 133 459 209 1754 Fragments arranged by size: 1754 1401 459 209 133 TatI w'GTAC_w Cuts at: 671 3471 671 Size: 2800 1156 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 180 212 3473 3505 180 Size: 32 3261 32 631 Fragments arranged by size: 3261 631 32 32 Tth111I GACn'n_nGTC Cuts at: 1753 1753 Size: 3956 XbaI T'CTAG_A Cuts at: 987 987 Size: 3956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1451 1451 Size: 3956 XmaI C'CCGG_G Cuts at: 976 976 Size: 3956 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BfrBI BmgBI BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI