(Linear) (Six-base) MAPSORT of: KIBB6195.seq Check: 8206 from: 1 to: 1989 KIBB6195.orf With 182 enzymes: SgfI * October 7, 2009 14:19 .. Acc65I G'GTAC_C Cuts at: 0 117 1989 Size: 117 1872 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 324 1178 1706 1711 1989 Size: 324 854 528 5 278 Fragments arranged by size: 854 528 324 278 5 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 253 1989 Size: 253 1736 AlwNI CAG_nnn'CTG Cuts at: 0 222 855 1519 1837 1989 Size: 222 633 664 318 152 Fragments arranged by size: 664 633 318 222 152 ApaI G_GGCC'C Cuts at: 0 1535 1662 1989 Size: 1535 127 327 Fragments arranged by size: 1535 327 127 ApoI r'AATT_y Cuts at: 0 1055 1989 Size: 1055 934 AvaI C'yCGr_G Cuts at: 0 147 1989 Size: 147 1842 AvrII C'CTAG_G Cuts at: 0 1215 1600 1989 Size: 1215 385 389 Fragments arranged by size: 1215 389 385 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 540 573 1989 Size: 540 33 1416 Fragments arranged by size: 1416 540 33 BanI G'GyrC_C Cuts at: 0 117 1497 1989 Size: 117 1380 492 Fragments arranged by size: 1380 492 117 BanII G_rGCy'C Cuts at: 0 422 1243 1437 1535 1662 1706 1850 1979 Size: 422 821 194 98 127 44 144 129 Cuts at: 1979 1989 Size: 10 Fragments arranged by size: 821 422 194 144 129 127 98 44 10 BbeI G_GCGC'C Cuts at: 0 1501 1989 Size: 1501 488 BbsI GAAGACnn'nnnn_ Cuts at: 0 86 514 544 1989 Size: 86 428 30 1445 Fragments arranged by size: 1445 428 86 30 BglI GCCn_nnn'nGGC Cuts at: 0 952 961 1432 1781 1989 Size: 952 9 471 349 208 Fragments arranged by size: 952 471 349 208 9 BglII A'GATC_T Cuts at: 0 335 1009 1989 Size: 335 674 980 Fragments arranged by size: 980 674 335 BlpI GC'TnA_GC Cuts at: 0 1880 1989 Size: 1880 109 Bme1580I G_kGCm'C Cuts at: 0 804 1202 1535 1662 1989 Size: 804 398 333 127 327 Fragments arranged by size: 804 398 333 327 127 BmrI ACTGGGnnnn_n' Cuts at: 0 14 521 1825 1989 Size: 14 507 1304 164 Fragments arranged by size: 1304 507 164 14 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 279 501 1869 1989 Size: 279 222 1368 120 Fragments arranged by size: 1368 279 222 120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 471 493 978 1057 1133 1315 1989 Size: 175 296 22 485 79 76 182 674 Fragments arranged by size: 674 485 296 182 175 79 76 22 Bpu10I CC'TnA_GC Cuts at: 0 1860 1989 Size: 1860 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1864 1989 Size: 1864 125 BsaI GGTCTCn'nnnn_ Cuts at: 0 1005 1075 1684 1989 Size: 1005 70 609 305 Fragments arranged by size: 1005 609 305 70 BsaHI Gr'CG_yC Cuts at: 0 1498 1989 Size: 1498 491 BsaWI w'CCGG_w Cuts at: 0 533 1989 Size: 533 1456 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1296 1326 1782 1812 1989 Size: 1296 30 456 30 177 Fragments arranged by size: 1296 456 177 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 490 1129 1620 1790 1842 1989 Size: 490 639 491 170 52 147 Fragments arranged by size: 639 491 490 170 147 52 BseYI C'CCAG_C Cuts at: 0 96 310 577 695 1201 1471 1534 1804 Size: 96 214 267 118 506 270 63 270 Cuts at: 1804 1929 1989 Size: 125 60 Fragments arranged by size: 506 270 270 267 214 125 118 96 63 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 359 1989 Size: 359 1630 BsiHKAI G_wGCw'C Cuts at: 0 1706 1850 1989 Size: 1706 144 139 Fragments arranged by size: 1706 144 139 BsmI GAATG_Cn' Cuts at: 0 465 1989 Size: 465 1524 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1149 1989 Size: 1149 840 Bsp1286I G_dGCh'C Cuts at: 0 422 804 1202 1243 1437 1535 1662 1706 Size: 422 382 398 41 194 98 127 44 Cuts at: 1706 1850 1979 1989 Size: 144 129 10 Fragments arranged by size: 422 398 382 194 144 129 127 98 44 41 10 BspEI T'CCGG_A Cuts at: 0 533 1989 Size: 533 1456 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 105 1989 Size: 105 1884 BsrDI GCAATG_nn' Cuts at: 0 1559 1989 Size: 1559 430 BstXI CCAn_nnnn'nTGG Cuts at: 0 888 1989 Size: 888 1101 BstYI r'GATC_y Cuts at: 0 218 335 1009 1118 1918 1989 Size: 218 117 674 109 800 71 Fragments arranged by size: 800 674 218 117 109 71 Bsu36I CC'TnA_GG Cuts at: 0 941 1696 1854 1989 Size: 941 755 158 135 Fragments arranged by size: 941 755 158 135 BtgI C'CryG_G Cuts at: 0 281 950 1229 1274 1505 1664 1989 Size: 281 669 279 45 231 159 325 Fragments arranged by size: 669 325 281 279 231 159 45 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1727 1762 1989 Size: 1727 35 227 Fragments arranged by size: 1727 227 35 DrdI GACnn_nn'nnGTC Cuts at: 0 892 1989 Size: 892 1097 EaeI y'GGCC_r Cuts at: 0 833 1989 Size: 833 1156 EarI CTCTTCn'nnn_ Cuts at: 0 520 656 1041 1682 1989 Size: 520 136 385 641 307 Fragments arranged by size: 641 520 385 307 136 EcoICRI GAG'CTC Cuts at: 0 1704 1848 1989 Size: 1704 144 141 Fragments arranged by size: 1704 144 141 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 324 471 493 978 1057 1133 1178 Size: 175 149 147 22 485 79 76 45 Cuts at: 1178 1315 1706 1711 1989 Size: 137 391 5 278 Fragments arranged by size: 485 391 278 175 149 147 137 79 76 45 22 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 1572 1989 Size: 1572 417 EcoO109I rG'GnC_Cy Cuts at: 0 194 885 1531 1625 1658 1659 1865 1989 Size: 194 691 646 94 33 1 206 124 Fragments arranged by size: 691 646 206 194 124 94 33 1 EcoRV GAT'ATC Cuts at: 0 66 318 1989 Size: 66 252 1671 Fragments arranged by size: 1671 252 66 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 354 1383 1989 Size: 354 1029 606 Fragments arranged by size: 1029 606 354 HaeII r_GCGC'y Cuts at: 0 1501 1989 Size: 1501 488 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 198 230 1782 1814 1989 Size: 198 32 1552 32 175 Fragments arranged by size: 1552 198 175 32 32 HincII GTy'rAC Cuts at: 0 278 1989 Size: 278 1711 KasI G'GCGC_C Cuts at: 0 1497 1989 Size: 1497 492 KpnI G_GTAC'C Cuts at: 0 121 1989 Size: 121 1868 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 140 1510 1762 1989 Size: 140 1370 252 227 Fragments arranged by size: 1370 252 227 140 MscI TGG'CCA Cuts at: 0 835 1989 Size: 835 1154 MslI CAynn'nnrTG Cuts at: 0 1969 1989 Size: 1969 20 MspA1I CmG'CkG Cuts at: 0 75 100 283 382 1345 1989 Size: 75 25 183 99 963 644 Fragments arranged by size: 963 644 183 99 75 25 NarI GG'CG_CC Cuts at: 0 1498 1989 Size: 1498 491 NcoI C'CATG_G Cuts at: 0 1664 1989 Size: 1664 325 NspI r_CATG'y Cuts at: 0 1066 1989 Size: 1066 923 PasI CC'CwG_GG Cuts at: 0 1020 1429 1549 1989 Size: 1020 409 120 440 Fragments arranged by size: 1020 440 409 120 PflMI CCAn_nnn'nTGG Cuts at: 0 75 749 1467 1989 Size: 75 674 718 522 Fragments arranged by size: 718 674 522 75 PfoI T'CCnGG_A Cuts at: 0 221 320 1989 Size: 221 99 1669 Fragments arranged by size: 1669 221 99 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1832 1864 1989 Size: 1832 32 125 Fragments arranged by size: 1832 125 32 PpuMI rG'GwC_Cy Cuts at: 0 194 885 1625 1865 1989 Size: 194 691 740 240 124 Fragments arranged by size: 740 691 240 194 124 PspOMI G'GGCC_C Cuts at: 0 1531 1658 1989 Size: 1531 127 331 Fragments arranged by size: 1531 331 127 PstI C_TGCA'G Cuts at: 0 1259 1989 Size: 1259 730 PvuII CAG'CTG Cuts at: 0 75 100 382 1989 Size: 75 25 282 1607 Fragments arranged by size: 1607 282 75 25 SacI G_AGCT'C Cuts at: 0 1706 1850 1989 Size: 1706 144 139 Fragments arranged by size: 1706 144 139 SacII CC_GC'GG Cuts at: 0 284 1989 Size: 284 1705 SanDI GG'GwC_CC Cuts at: 0 1865 1989 Size: 1865 124 SapI GCTCTTCn'nnn_ Cuts at: 0 656 1041 1989 Size: 656 385 948 Fragments arranged by size: 948 656 385 ScaI AGT'ACT Cuts at: 0 1305 1989 Size: 1305 684 SexAI A'CCwGG_T Cuts at: 0 887 1989 Size: 887 1102 SfcI C'TryA_G Cuts at: 0 13 1255 1589 1989 Size: 13 1242 334 400 Fragments arranged by size: 1242 400 334 13 SfiI GGCCn_nnn'nGGCC Cuts at: 0 952 961 1989 Size: 952 9 1028 Fragments arranged by size: 1028 952 9 SfoI GGC'GCC Cuts at: 0 1499 1989 Size: 1499 490 SmlI C'TyrA_G Cuts at: 0 1843 1989 Size: 1843 146 SpeI A'CTAG_T Cuts at: 0 448 1989 Size: 448 1541 StuI AGG'CCT Cuts at: 0 484 946 1381 1989 Size: 484 462 435 608 Fragments arranged by size: 608 484 462 435 StyI C'CwwG_G Cuts at: 0 542 881 1215 1600 1664 1989 Size: 542 339 334 385 64 325 Fragments arranged by size: 542 385 339 334 325 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1057 1518 1989 Size: 1057 461 471 Fragments arranged by size: 1057 471 461 TatI w'GTAC_w Cuts at: 0 143 680 1303 1989 Size: 143 537 623 686 Fragments arranged by size: 686 623 537 143 Tth111I GACn'n_nGTC Cuts at: 0 1794 1989 Size: 1794 195 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 318 888 957 1468 1989 Size: 318 570 69 511 521 Fragments arranged by size: 570 521 511 318 69 XmnI GAAnn'nnTTC Cuts at: 0 356 1989 Size: 356 1633 Enzymes that do cut: Acc65I AcuI AlfI AlwNI ApaI ApoI AvaI AvrII BaeI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BstXI BstYI Bsu36I BtgI CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI ScaI SexAI SfcI SfiI SfoI SmlI SpeI StuI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI BamHI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI DraI DraIII EagI EciI EcoRI FseI FspI FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI TstI XbaI XhoI XmaI ZraI