(Circular) (Six-base) MAPSORT of: pF1KB3491.seq Check: 721 from: 1 to: 5147 pF1KB3491 5147 bp With 182 enzymes: SgfI * April 24, 2009 13:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1681 1737 1681 Size: 56 5091 AccI GT'mk_AC Cuts at: 2185 2185 Size: 5147 Acc65I G'GTAC_C Cuts at: 2163 2163 Size: 5147 AclI AA'CG_TT Cuts at: 4987 4987 Size: 5147 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 932 1012 2972 3404 3757 4419 932 Size: 80 1960 432 353 662 1660 Fragments arranged by size: 1960 1660 662 432 353 80 AfeI AGC'GCT Cuts at: 232 232 Size: 5147 AflIII A'CryG_T Cuts at: 1650 2499 4299 4580 1650 Size: 849 1800 281 2217 Fragments arranged by size: 2217 1800 849 281 AhdI GACnn_n'nnGTC Cuts at: 1501 1501 Size: 5147 AleI CACnn'nnGTG Cuts at: 1135 2015 1135 Size: 880 4267 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1769 1769 Size: 5147 AlwNI CAG_nnn'CTG Cuts at: 576 724 1114 2452 3890 576 Size: 148 390 1338 1438 1833 Fragments arranged by size: 1833 1438 1338 390 148 ApaI G_GGCC'C Cuts at: 1826 1826 Size: 5147 ApaLI G'TGCA_C Cuts at: 3985 3985 Size: 5147 ApoI r'AATT_y Cuts at: 446 499 553 862 1354 1535 2062 2083 2151 Size: 53 54 309 492 181 527 21 68 Cuts at: 2151 4500 4756 446 Size: 2349 256 837 Fragments arranged by size: 2349 837 527 492 309 256 181 68 54 53 21 AseI AT'TA_AT Cuts at: 20 20 Size: 5147 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5147 AvaI C'yCGr_G Cuts at: 1853 2167 1853 Size: 314 4833 BamHI G'GATC_C Cuts at: 141 2172 141 Size: 2031 3116 BanI G'GyrC_C Cuts at: 1555 2163 2577 2825 2860 1555 Size: 608 414 248 35 3842 Fragments arranged by size: 3842 608 414 248 35 BanII G_rGCy'C Cuts at: 1108 1826 2161 3191 1108 Size: 718 335 1030 3064 Fragments arranged by size: 3064 1030 718 335 BbeI G_GCGC'C Cuts at: 2581 2829 2581 Size: 248 4899 BbsI GAAGACnn'nnnn_ Cuts at: 224 1165 224 Size: 941 4206 BbvCI CC'TCA_GC Cuts at: 995 1827 995 Size: 832 4315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 758 792 758 Size: 34 5113 BciVI GTATCCnnnnn_n' Cuts at: 1151 2014 3045 4101 5147 1151 Size: 863 1031 1056 1046 1151 Fragments arranged by size: 1151 1056 1046 1031 863 BclI T'GATC_A Cuts at: 300 2007 2667 300 Size: 1707 660 2780 Fragments arranged by size: 2780 1707 660 BfrBI ATG'CAT Cuts at: 769 769 Size: 5147 BglI GCCn_nnn'nGGC Cuts at: 991 1065 991 Size: 74 5073 BglII A'GATC_T Cuts at: 676 1149 2662 4699 676 Size: 473 1513 2037 1124 Fragments arranged by size: 2037 1513 1124 473 BlpI GC'TnA_GC Cuts at: 2259 2259 Size: 5147 Bme1580I G_kGCm'C Cuts at: 1826 2772 2865 3989 1826 Size: 946 93 1124 2984 Fragments arranged by size: 2984 1124 946 93 BmrI ACTGGGnnnn_n' Cuts at: 498 1869 2359 2440 2775 4919 498 Size: 1371 490 81 335 2144 726 Fragments arranged by size: 2144 1371 726 490 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3514 3514 Size: 5147 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 350 553 746 1054 1136 1292 1424 1436 1499 Size: 203 193 308 82 156 132 12 63 Cuts at: 1499 1700 1894 350 Size: 201 194 3603 Fragments arranged by size: 3603 308 203 201 194 193 156 132 82 63 12 Bpu10I CC'TnA_GC Cuts at: 995 1827 995 Size: 832 4315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2323 3202 3916 4214 2323 Size: 879 714 298 3256 Fragments arranged by size: 3256 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 4851 4851 Size: 5147 BsaAI yAC'GTr Cuts at: 1653 2500 1653 Size: 847 4300 BsaBI GATnn'nnATC Cuts at: 6 2661 4698 6 Size: 2655 2037 455 Fragments arranged by size: 2655 2037 455 BsaHI Gr'CG_yC Cuts at: 2578 2826 3528 5024 2578 Size: 248 702 1496 2701 Fragments arranged by size: 2701 1496 702 248 BsaWI w'CCGG_w Cuts at: 527 2336 2857 3535 3946 4093 4367 527 Size: 1809 521 678 411 147 274 1307 Fragments arranged by size: 1809 1307 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1195 1225 1195 Size: 30 5117 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 718 718 Size: 5147 BseYI C'CCAG_C Cuts at: 1698 2572 3330 3995 4403 1698 Size: 874 758 665 408 2442 Fragments arranged by size: 2442 874 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 142 298 142 Size: 156 4991 BsiEI CG_ry'CG Cuts at: 104 2689 2735 3965 104 Size: 2585 46 1230 1286 Fragments arranged by size: 2585 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 366 1523 2161 2939 3129 3989 366 Size: 1157 638 778 190 860 1524 Fragments arranged by size: 1524 1157 860 778 638 190 BsmI GAATG_Cn' Cuts at: 1172 1172 Size: 5147 Bsp1286I G_dGCh'C Cuts at: 366 1108 1523 1826 2161 2772 2865 2939 3129 Size: 742 415 303 335 611 93 74 190 Cuts at: 3129 3191 3989 366 Size: 62 798 1524 Fragments arranged by size: 1524 798 742 611 415 335 303 190 93 74 62 BspHI T'CATG_A Cuts at: 147 3579 147 Size: 3432 1715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1681 1737 2197 2411 2713 3094 1681 Size: 56 460 214 302 381 3734 Fragments arranged by size: 3734 460 381 302 214 56 BsrBI CCG'CTC Cuts at: 50 3439 3493 4977 5142 50 Size: 3389 54 1484 165 55 Fragments arranged by size: 3389 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1617 1979 3059 1617 Size: 362 1080 3705 Fragments arranged by size: 3705 1080 362 BsrFI r'CCGG_y Cuts at: 3145 3326 3145 Size: 181 4966 BsrGI T'GTAC_A Cuts at: 1211 1211 Size: 5147 BssSI C'ACGA_G Cuts at: 3418 4126 3418 Size: 708 4439 BstAPI GCAn_nnn'nTGC Cuts at: 2524 2524 Size: 5147 BstBI TT'CG_AA Cuts at: 3508 3508 Size: 5147 BstEII G'GTnAC_C Cuts at: 666 1713 666 Size: 1047 4100 BstXI CCAn_nnnn'nTGG Cuts at: 1446 1446 Size: 5147 BstYI r'GATC_y Cuts at: 1 141 676 1149 2172 2639 2662 2997 3243 Size: 140 535 473 1023 467 23 335 246 Cuts at: 3243 3549 3561 3647 4699 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1052 1023 535 473 467 449 335 306 246 140 86 23 12 BtgI C'CryG_G Cuts at: 107 1490 1685 107 Size: 1383 195 3569 Fragments arranged by size: 3569 1383 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3097 3278 3441 3097 Size: 181 163 4803 Fragments arranged by size: 4803 181 163 BtsI GCAGTG_nn' Cuts at: 1130 1142 2132 2390 1130 Size: 12 990 258 3887 Fragments arranged by size: 3887 990 258 12 DraI TTT'AAA Cuts at: 1664 2146 1664 Size: 482 4665 DrdI GACnn_nn'nnGTC Cuts at: 2853 4197 2853 Size: 1344 3803 EaeI y'GGCC_r Cuts at: 1764 2732 2906 3297 3324 4669 1764 Size: 968 174 391 27 1345 2242 Fragments arranged by size: 2242 1345 968 391 174 27 EagI C'GGCC_G Cuts at: 2732 2732 Size: 5147 EarI CTCTTCn'nnn_ Cuts at: 1359 3170 3380 1359 Size: 1811 210 3126 Fragments arranged by size: 3126 1811 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3355 4097 4243 4957 3355 Size: 742 146 714 3545 Fragments arranged by size: 3545 742 714 146 EcoICRI GAG'CTC Cuts at: 2159 2159 Size: 5147 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 350 553 746 932 1012 1054 1136 1292 1424 Size: 203 193 186 80 42 82 156 132 Cuts at: 1424 1436 1499 1700 1894 2972 3404 3757 4419 Size: 12 63 201 194 1078 432 353 662 Cuts at: 4419 350 Size: 1078 Fragments arranged by size: 1078 1078 662 432 353 203 201 194 193 186 156 132 82 80 63 42 12 EcoNI CCTnn'n_nnAGG Cuts at: 1049 1049 Size: 5147 EcoO109I rG'GnC_Cy Cuts at: 1001 1495 1822 1823 2286 1001 Size: 494 327 1 463 3862 Fragments arranged by size: 3862 494 463 327 1 EcoRI G'AATT_C Cuts at: 553 2151 553 Size: 1598 3549 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1260 3166 1260 Size: 1906 3241 FspI TGC'GCA Cuts at: 2928 2928 Size: 5147 HaeII r_GCGC'y Cuts at: 234 957 1511 2581 2829 4059 4830 234 Size: 723 554 1070 248 1230 771 551 Fragments arranged by size: 1230 1070 771 723 554 551 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 312 344 627 659 719 751 1487 1519 1836 Size: 32 283 32 60 32 736 32 317 Cuts at: 1836 1868 2012 2044 2989 3019 3021 3051 312 Size: 32 144 32 945 30 2 30 2408 Fragments arranged by size: 2408 945 736 317 283 144 60 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2186 2186 Size: 5147 KasI G'GCGC_C Cuts at: 2577 2825 2577 Size: 248 4899 KpnI G_GTAC'C Cuts at: 2167 2167 Size: 5147 MfeI C'AATT_G Cuts at: 580 580 Size: 5147 MluI A'CGCG_T Cuts at: 4580 4580 Size: 5147 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 531 3906 4090 531 Size: 3375 184 1588 Fragments arranged by size: 3375 1588 184 MscI TGG'CCA Cuts at: 1766 2908 1766 Size: 1142 4005 MslI CAynn'nnrTG Cuts at: 1135 2015 3136 3263 1135 Size: 880 1121 127 3019 Fragments arranged by size: 3019 1121 880 127 MspA1I CmG'CkG Cuts at: 724 1678 1702 1914 1951 2258 2572 2932 3716 Size: 954 24 212 37 307 314 360 784 Cuts at: 3716 3961 724 Size: 245 1910 Fragments arranged by size: 1910 954 784 360 314 307 245 212 37 24 NaeI GCC'GGC Cuts at: 3328 3328 Size: 5147 NarI GG'CG_CC Cuts at: 2578 2826 2578 Size: 248 4899 NcoI C'CATG_G Cuts at: 107 1685 107 Size: 1578 3569 NgoMIV G'CCGG_C Cuts at: 3326 3326 Size: 5147 NsiI A_TGCA'T Cuts at: 771 771 Size: 5147 NspI r_CATG'y Cuts at: 592 687 2200 2700 4303 592 Size: 95 1513 500 1603 1436 Fragments arranged by size: 1603 1513 1436 500 95 PciI A'CATG_T Cuts at: 4299 4299 Size: 5147 PfoI T'CCnGG_A Cuts at: 569 1523 1811 569 Size: 954 288 3905 Fragments arranged by size: 3905 954 288 PmeI GTTT'AAAC Cuts at: 2146 2146 Size: 5147 PmlI CAC'GTG Cuts at: 1653 1653 Size: 5147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 3593 Size: 32 5115 PpuMI rG'GwC_Cy Cuts at: 1495 1495 Size: 5147 PshAI GACnn'nnGTC Cuts at: 1038 1038 Size: 5147 PspOMI G'GGCC_C Cuts at: 1822 1822 Size: 5147 PspXI vC'TCGA_Gb Cuts at: 1853 1853 Size: 5147 PstI C_TGCA'G Cuts at: 1137 1748 1851 2194 2879 1137 Size: 611 103 343 685 3405 Fragments arranged by size: 3405 685 611 343 103 PvuI CG_AT'CG Cuts at: 104 104 Size: 5147 PvuII CAG'CTG Cuts at: 724 1678 1702 2572 2932 724 Size: 954 24 870 360 2939 Fragments arranged by size: 2939 954 870 360 24 RsrII CG'GwC_CG Cuts at: 3342 3342 Size: 5147 SacI G_AGCT'C Cuts at: 2161 2161 Size: 5147 SalI G'TCGA_C Cuts at: 2184 2184 Size: 5147 SanDI GG'GwC_CC Cuts at: 1495 1495 Size: 5147 SapI GCTCTTCn'nnn_ Cuts at: 3170 3380 3170 Size: 210 4937 SbfI CC_TGCA'GG Cuts at: 2194 2194 Size: 5147 ScaI AGT'ACT Cuts at: 183 4664 183 Size: 4481 666 SexAI A'CCwGG_T Cuts at: 743 743 Size: 5147 SfcI C'TryA_G Cuts at: 33 169 1133 1145 1272 1744 1847 2190 2875 Size: 136 964 12 127 472 103 343 685 Cuts at: 2875 3843 4034 33 Size: 968 191 1146 Fragments arranged by size: 1146 968 964 685 472 343 191 136 127 103 12 SfoI GGC'GCC Cuts at: 2579 2827 2579 Size: 248 4899 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5147 SmaI CCC'GGG Cuts at: 2169 2169 Size: 5147 SmlI C'TyrA_G Cuts at: 1853 2302 3217 3931 4193 1853 Size: 449 915 714 262 2807 Fragments arranged by size: 2807 915 714 449 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5147 SphI G_CATG'C Cuts at: 2200 2700 2200 Size: 500 4647 StuI AGG'CCT Cuts at: 1059 1940 1059 Size: 881 4266 StyI C'CwwG_G Cuts at: 107 308 1685 1889 2281 2635 107 Size: 201 1377 204 392 354 2619 Fragments arranged by size: 2619 1377 392 354 204 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2730 2863 3322 3531 2730 Size: 133 459 209 4346 Fragments arranged by size: 4346 459 209 133 TatI w'GTAC_w Cuts at: 181 1211 4662 181 Size: 1030 3451 666 Fragments arranged by size: 3451 1030 666 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4664 4696 4664 Size: 32 5115 Tth111I GACn'n_nGTC Cuts at: 2944 2944 Size: 5147 XbaI T'CTAG_A Cuts at: 2178 2178 Size: 5147 XcmI CCAnnnn_n'nnnnTGG Cuts at: 381 1183 2642 381 Size: 802 1459 2886 Fragments arranged by size: 2886 1459 802 XhoI C'TCGA_G Cuts at: 1853 1853 Size: 5147 XmaI C'CCGG_G Cuts at: 2167 2167 Size: 5147 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AflII AgeI AloI AscI AvrII BaeI BmgBI BmtI BsiWI BsmBI BspEI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI PacI PasI PflMI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI