Restriction map for pF1KB9950
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# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AspI        AviII       
AvrII       BanI        BbeI        BbvCI       BcgI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        DsaI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       EcoRV       FauI        
FseI        FspI        HaeII       HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AciI         1164  CCGC                 CCGC(-3/-1)
AcyI          213  GACGCC               G[GA]^CG[CT]C
AhdI         1201  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1500  CCTGAGG              CC^T[ACGT]AGG
AspEI        1201  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1275  TGGCCA               TGG^CCA
BanII        1347  GGGCTC               G[GA]GC[CT]^C
BbrPI        1048  CACGTG               CAC^GTG
BciVI           3  GGATAC               GTATCC(6/5)
BcnI         1602  CCGGG                CC^[GC]GG
BglII        1638  AGATCT               A^GATCT
BmyI         1347  GGGCTC               G[AGT]GC[ACT]^C
BsaAI        1048  CACGTG               [CT]AC^GT[GA]
BsaBI         908  GATCACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         213  GACGCC               G[GA]^CG[CT]C
BsaI         1872  GGTCTC               GGTCTC(1/5)
Bsp1286I     1347  GGGCTC               G[AGT]GC[ACT]^C
BspMI         344  ACCTGC               ACCTGC(4/8)
BsrDI        1478  GCAATG               GCAATG(2/0)
BsrFI        1895  ACCGGC               [GA]^CCGG[CT]
BssSI        1948  CACGAG               CACGAG(-5/-1)
BstAPI       1478  GCAATGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        334  GGTAACC              G^GT[ACGT]ACC
BstPI         334  GGTAACC              G^GT[ACGT]ACC
BstXI        1723  CCAGTCAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1500  CCTGAGG              CC^T[ACGT]AGG
CfoI          640  GCGC                 GCG^C
Cfr10I       1895  ACCGGC               [GA]^CCGG[CT]
DraII        1193  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII       1168  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1690  GACCAGTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1275  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1201  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1500  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1193  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       334  GGTAACC              G^GT[ACGT]ACC
EcoT14I       706  CCAAGG               C^C[AT][AT]GG
EcoT22I       275  ATGCAT               ATGCA^T
HhaI          640  GCGC                 GCG^C
Hin1I         213  GACGCC               G[GA]^CG[CT]C
HinP1I        640  GCGC                 G^CGC
HincII       1176  GTCAAC               GT[CT]^[GA]AC
HindII       1176  GTCAAC               GT[CT]^[GA]AC
HindIII      1114  AAGCTT               A^AGCTT
MamI          908  GATCACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1275  TGGCCA               TGG^CCA
MscI         1275  TGGCCA               TGG^CCA
MslI         1054  CATACAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI         1602  CCGGG                CC^[GC]GG
NdeI          443  CATATG               CA^TATG
NsiI          275  ATGCAT               ATGCA^T
NspI          692  ACATGC               [GA]CATG^[CT]
PaeR7I        202  CTCGAG               C^TCGAG
PflMI         342  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1048  CACGTG               CAC^GTG
PmlI         1048  CACGTG               CAC^GTG
PpuMI        1193  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          461  CTGCAG               CTGCA^G
SmlI          202  CTCGAG               C^T[CT][GA]AG
StyI          706  CCAAGG               C^C[AT][AT]GG
Van91I        342  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI           18  TCTAGA               T^CTAGA
XhoI          202  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwNI         527  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1938  CAGCTGCTG           
AvaI          202  CTCGAG               C^[CT]CG[GA]G
              521  CTCGGG              
AvaII        1194  GGTCC                G^G[AT]CC
             1290  GGTCC               
BamHI         157  GGATCC               G^GATCC
             1796  GGATCC              
BbsI          712  GAAGAC               GAAGAC(2/6)
             1989  GTCTTC              
BclI         1151  TGATCA               T^GATCA
             1337  TGATCA              
BpmI          903  CTCCAG               CTGGAG(16/14)
             1739  CTCCAG              
BpuAI         712  GAAGAC               GAAGAC(2/6)
             1989  GTCTTC              
BseRI         205  GAGGAG               GAGGAG(10/8)
             1503  GAGGAG              
BsmAI         225  GAGAC                GTCTC(1/5)
             1873  GTCTC               
BsoBI         202  CTCGAG               C^[CT]CG[GA]G
              521  CTCGGG              
Bsp1407I      986  TGTACA               T^GTACA
             1934  TGTACA              
BsrGI         986  TGTACA               T^GTACA
             1934  TGTACA              
EcoRI         832  GAATTC               G^AATTC
             1366  GAATTC              
FbaI         1151  TGATCA               T^GATCA
             1337  TGATCA              
HapII        1602  CCGG                 C^CGG
             1896  CCGG                
HgaI          213  GACGC                GACGC(5/10)
             1965  GCGTC               
HpaII        1602  CCGG                 C^CGG
             1896  CCGG                
HphI           64  TCACC                GGTGA(8/7)
             1587  TCACC               
MaeII        1049  ACGT                 A^CGT
             1781  ACGT                
MspA1I       1294  CAGCTG               C[AC]G^C[GT]G
             1938  CAGCTG              
MspI         1602  CCGG                 C^CGG
             1896  CCGG                
PvuII        1294  CAGCTG               CAG^CTG
             1938  CAGCTG              
SfcI          218  CTGTAG               C^T[GA][CT]AG
              461  CTGCAG              
SspBI         986  TGTACA               T^GTACA
             1934  TGTACA              
TaqI          203  TCGA                 T^CGA
              970  TCGA                
TthHB8I       203  TCGA                 T^CGA
              970  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     BbvI        BfaI        BsaJI       BsiYI       
BslI        BsmFI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       XmnI        
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