Restriction map for pF1KB9950
 (Linear) (Six-base) MAPSORT of: KIBB9950.seq  Check: 161  from: 1  to: 2025



KIBB9950.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     353    2025 
   Size:        353    1672
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     875    1888    2025 
   Size:        532     343    1013     137

  Fragments arranged by size: 

                1013     532     343     137
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1206    2025 
   Size:       1206     819
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1076    2025 
   Size:       1076     949
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     370     402    1704    1736    2025 
   Size:        370      32    1302      32     289

  Fragments arranged by size: 

                1302     370     289      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     532    1943    2025 
   Size:        532    1411      82

  Fragments arranged by size: 

                1411     532      82
 
ApoI r'AATT_y

Cuts at:      0     832     966    1366    1420    2025 
   Size:        832     134     400      54     605

  Fragments arranged by size: 

                 832     605     400     134      54
 
AvaI C'yCGr_G

Cuts at:      0     202     521    2025 
   Size:        202     319    1504

  Fragments arranged by size: 

                1504     319     202
 
BamHI G'GATC_C

Cuts at:      0     157    1796    2025 
   Size:        157    1639     229

  Fragments arranged by size: 

                1639     229     157
 
BanII G_rGCy'C

Cuts at:      0    1351    2025 
   Size:       1351     674
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     719    1982    2025 
   Size:        719    1263      43

  Fragments arranged by size: 

                1263     719      43
 
BclI T'GATC_A

Cuts at:      0    1151    1337    2025 
   Size:       1151     186     688

  Fragments arranged by size: 

                1151     688     186
 
BfrBI ATG'CAT

Cuts at:      0     277    2025 
   Size:        277    1748
 
BglII A'GATC_T

Cuts at:      0    1638    2025 
   Size:       1638     387
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     888    1724    2025 
   Size:        888     836     301

  Fragments arranged by size: 

                 888     836     301
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1878    2025 
   Size:       1878     147
 
BsaAI yAC'GTr

Cuts at:      0    1050    2025 
   Size:       1050     975
 
BsaBI GATnn'nnATC

Cuts at:      0     912    2025 
   Size:        912    1113
 
BsaHI Gr'CG_yC

Cuts at:      0     214    2025 
   Size:        214    1811
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     592     622    2025 
   Size:        592      30    1403

  Fragments arranged by size: 

                1403     592      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     220    1518    2025 
   Size:        220    1298     507

  Fragments arranged by size: 

                1298     507     220
 
BseYI C'CCAG_C

Cuts at:      0     611     677    1266    1296    1532    2025 
   Size:        611      66     589      30     236     493

  Fragments arranged by size: 

                 611     589     493     236      66      30
 
Bsp1286I G_dGCh'C

Cuts at:      0    1351    2025 
   Size:       1351     674
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     353    2025 
   Size:        353    1672
 
BsrDI GCAATG_nn'

Cuts at:      0    1485    2025 
   Size:       1485     540
 
BsrFI r'CCGG_y

Cuts at:      0    1895    2025 
   Size:       1895     130
 
BsrGI T'GTAC_A

Cuts at:      0     986    1934    2025 
   Size:        986     948      91

  Fragments arranged by size: 

                 986     948      91
 
BssSI C'ACGA_G

Cuts at:      0    1948    2025 
   Size:       1948      77
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1484    2025 
   Size:       1484     541
 
BstEII G'GTnAC_C

Cuts at:      0     334    2025 
   Size:        334    1691
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1730    2025 
   Size:       1730     295
 
BstYI r'GATC_y

Cuts at:      0     157    1638    1796    2025 
   Size:        157    1481     158     229

  Fragments arranged by size: 

                1481     229     158     157
 
Bsu36I CC'TnA_GG

Cuts at:      0    1501    2025 
   Size:       1501     524
 
BtsI GCAGTG_nn'

Cuts at:      0     768    2025 
   Size:        768    1257
 
DraIII CAC_nnn'GTG

Cuts at:      0    1173    2025 
   Size:       1173     852
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1696    2025 
   Size:       1696     329
 
EaeI y'GGCC_r

Cuts at:      0    1275    2025 
   Size:       1275     750
 
EarI CTCTTCn'nnn_

Cuts at:      0     281     467     854    1238    2025 
   Size:        281     186     387     384     787

  Fragments arranged by size: 

                 787     387     384     281     186
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1153    2025 
   Size:       1153     872
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     875     888    1724    1888    2025 
   Size:        532     343      13     836     164     137

  Fragments arranged by size: 

                 836     532     343     164     137      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1194    2025 
   Size:       1194     831
 
EcoRI G'AATT_C

Cuts at:      0     832    1366    2025 
   Size:        832     534     659

  Fragments arranged by size: 

                 832     659     534
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      46     103     277    2025 
   Size:         46      57     174    1748

  Fragments arranged by size: 

                1748     174      57      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     282     314     416     448     538     570     574 
   Size:         27     255      32     102      32      90      32       4

Cuts at:    574     606     640     672    1192    1224    2025 
   Size:         32      34      32     520      32     801

  Fragments arranged by size: 

                 801     520     255     102      90      34      32      32
                  32      32      32      32      27       4
 
HincII GTy'rAC

Cuts at:      0    1178    2025 
   Size:       1178     847
 
HindIII A'AGCT_T

Cuts at:      0    1114    2025 
   Size:       1114     911
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1689    2025 
   Size:       1689     336
 
MscI TGG'CCA

Cuts at:      0    1277    2025 
   Size:       1277     748
 
MslI CAynn'nnrTG

Cuts at:      0    1058    2025 
   Size:       1058     967
 
MspA1I CmG'CkG

Cuts at:      0    1296    1940    2025 
   Size:       1296     644      85

  Fragments arranged by size: 

                1296     644      85
 
NdeI CA'TA_TG

Cuts at:      0     444    2025 
   Size:        444    1581
 
NsiI A_TGCA'T

Cuts at:      0     279    2025 
   Size:        279    1746
 
NspI r_CATG'y

Cuts at:      0     696    2025 
   Size:        696    1329
 
PasI CC'CwG_GG

Cuts at:      0    1212    1878    2025 
   Size:       1212     666     147

  Fragments arranged by size: 

                1212     666     147
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     348    2025 
   Size:        348    1677
 
PmlI CAC'GTG

Cuts at:      0    1050    2025 
   Size:       1050     975
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      17      49     911     943    2025 
   Size:         17      32     862      32    1082

  Fragments arranged by size: 

                1082     862      32      32      17
 
PpuMI rG'GwC_Cy

Cuts at:      0    1194    2025 
   Size:       1194     831
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1060    1092    2025 
   Size:       1060      32     933

  Fragments arranged by size: 

                1060     933      32
 
PstI C_TGCA'G

Cuts at:      0     465    2025 
   Size:        465    1560
 
PvuII CAG'CTG

Cuts at:      0    1296    1940    2025 
   Size:       1296     644      85

  Fragments arranged by size: 

                1296     644      85
 
SfcI C'TryA_G

Cuts at:      0     218     461    2025 
   Size:        218     243    1564

  Fragments arranged by size: 

                1564     243     218
 
SmlI C'TyrA_G

Cuts at:      0     202    2025 
   Size:        202    1823
 
StyI C'CwwG_G

Cuts at:      0     706    2025 
   Size:        706    1319
 
TatI w'GTAC_w

Cuts at:      0     986    1934    2025 
   Size:        986     948      91

  Fragments arranged by size: 

                 986     948      91
 
XbaI T'CTAG_A

Cuts at:      0      18    2025 
   Size:         18    2007
 
XhoI C'TCGA_G

Cuts at:      0     202    2025 
   Size:        202    1823
 
XmnI GAAnn'nnTTC

Cuts at:      0      26      29    1370    2003    2025 
   Size:         26       3    1341     633      22

  Fragments arranged by size: 

                1341     633      26      22       3
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AlfI     AloI    AlwNI     ApoI     AvaI
    BamHI    BanII     BbsI     BclI    BfrBI    BglII     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI Bsp1286I    BspMI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtsI   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     MmeI     MscI
     MslI   MspA1I     NdeI     NsiI     NspI     PasI    PflMI     PmlI
     PpiI    PpuMI     PsrI     PstI    PvuII     SfcI     SmlI     StyI
     TatI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BanI     BbeI    BbvCI     BcgI    BciVI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
   BssHII    BstBI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
     EagI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PciI     PfoI     PmeI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XcmI     XmaI     ZraI
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