################################## # NO CUT ENZYMES ( 208 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI TseI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AcsI 72 AAATTC [GA]^AATT[CT] ApoI 72 AAATTC [GA]^AATT[CT] AvaII 96 GGTCC G^G[AT]CC BbsI 5 GTCTTC GAAGAC(2/6) BfaI 23 CTAG C^TAG BglI 107 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 5 GTCTTC GAAGAC(2/6) BsmAI 231 GAGAC GTCTC(1/5) BsmBI 231 GAGACG CGTCTC(1/5) Bsp1407I 212 TGTACA T^GTACA BsrGI 212 TGTACA T^GTACA BsrI 55 CCAGT ACTGG(1/-1) Cac8I 103 GCTTGC GC[ACGT]^[ACGT]GC Cfr13I 96 GGTCC G^G[ACGT]CC DraII 95 GGGTCCT [GA]G^G[ACGT]CC[CT] EarI 228 GAAGAG CTCTTC(1/4) EcoO109I 95 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoT22I 185 ATGCAT ATGCA^T HinfI 3 GAGTC G^A[ACGT]TC Ksp632I 228 GAAGAG CTCTTC(1/4) MaeI 23 CTAG C^TAG MaeIII 26 GTGAC ^GT[ACGT]AC MwoI 107 GCCTGGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 120 CATG CATG^ NlaIV 95 GGGTCC GG[ACGT]^[ACGT]CC NsiI 185 ATGCAT ATGCA^T PleI 3 GAGTC GAGTC(4/5) PpuMI 95 GGGTCCT [GA]G^G[AT]CC[CT] Sau96I 96 GGTCC G^G[ACGT]CC SfaNI 184 GATGC GCATC(5/9) SspBI 212 TGTACA T^GTACA TaqI 90 TCGA T^CGA Tsp45I 26 GTGAC ^GT[GC]AC TthHB8I 90 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AfaI 213 GTAC GT^AC 221 GTAC BpmI 77 CTGGAG CTGGAG(16/14) 133 CTGGAG BsaJI 131 CCCTGG C^C[ACGT][ACGT]GG 195 CCGAGG MnlI 92 GAGG CCTC(7/6) 197 GAGG MseI 34 TTAA T^TAA 70 TTAA RsaI 213 GTAC GT^AC 221 GTAC Tru9I 34 TTAA T^TAA 70 TTAA Tsp509I 68 AATT ^AATT 73 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### BssKI BstF5I BstNI EcoRII FokI MboII MvaI ScrFI