(Linear) (Six-base) MAPSORT of: KIBB6713.seq  Check: 6445  from: 1  to: 240



KIBB6713.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     240 
   Size:        187      53
 
ApoI r'AATT_y

Cuts at:      0      72     240 
   Size:         72     168
 
BfrBI ATG'CAT

Cuts at:      0     187     240 
   Size:        187      53
 
BglI GCCn_nnn'nGGC

Cuts at:      0     113     240 
   Size:        113     127
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     154     240 
   Size:         98      56      86

  Fragments arranged by size: 

                  98      86      56
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     225     240 
   Size:        225      15
 
BsrGI T'GTAC_A

Cuts at:      0     212     240 
   Size:        212      28
 
EarI CTCTTCn'nnn_

Cuts at:      0     223     240 
   Size:        223      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     154     187     240 
   Size:         98      56      33      53

  Fragments arranged by size: 

                  98      56      53      33
 
EcoO109I rG'GnC_Cy

Cuts at:      0      96     240 
   Size:         96     144
 
NsiI A_TGCA'T

Cuts at:      0     189     240 
   Size:        189      51
 
PpuMI rG'GwC_Cy

Cuts at:      0      96     240 
   Size:         96     144
 
TatI w'GTAC_w

Cuts at:      0     212     220     240 
   Size:        212       8      20

  Fragments arranged by size: 

                 212      20       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     191     223     240 
   Size:        191      32      17

  Fragments arranged by size: 

                 191      32      17
 

 Enzymes that do cut:

     AcuI     ApoI    BfrBI     BglI     BpmI    BsmBI    BsrGI     EarI
  Eco57MI EcoO109I     NsiI    PpuMI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI