(Circular) (Six-base) MAPSORT of: pF1KB6974.seq Check: 4527 from: 1 to: 3842 pF1KB6974 3842 bp With 182 enzymes: SgfI * October 7, 2009 14:23 .. AatII G_ACGT'C Cuts at: 223 223 Size: 3842 AccI GT'mk_AC Cuts at: 880 880 Size: 3842 Acc65I G'GTAC_C Cuts at: 858 858 Size: 3842 AclI AA'CG_TT Cuts at: 3682 3682 Size: 3842 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 401 1667 2099 2846 3114 401 Size: 1266 432 747 268 1129 Fragments arranged by size: 1266 1129 747 432 268 AflIII A'CryG_T Cuts at: 1194 2298 3275 1194 Size: 1104 977 1761 Fragments arranged by size: 1761 1104 977 AgeI A'CCGG_T Cuts at: 2230 2230 Size: 3842 AlwNI CAG_nnn'CTG Cuts at: 1147 2714 1147 Size: 1567 2275 ApaLI G'TGCA_C Cuts at: 2612 2612 Size: 3842 ApoI r'AATT_y Cuts at: 555 846 3195 3451 555 Size: 291 2349 256 946 Fragments arranged by size: 2349 946 291 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3842 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3842 AvaI C'yCGr_G Cuts at: 862 862 Size: 3842 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 527 560 527 Size: 33 3809 BamHI G'GATC_C Cuts at: 867 867 Size: 3842 BanI G'GyrC_C Cuts at: 858 1272 1520 1555 858 Size: 414 248 35 3145 Fragments arranged by size: 3145 414 248 35 BanII G_rGCy'C Cuts at: 856 1886 856 Size: 1030 2812 BbeI G_GCGC'C Cuts at: 1276 1524 1276 Size: 248 3594 BciVI GTATCCnnnnn_n' Cuts at: 1740 2501 3842 1740 Size: 761 1341 1740 Fragments arranged by size: 1740 1341 761 BclI T'GATC_A Cuts at: 1362 1362 Size: 3842 BglII A'GATC_T Cuts at: 1357 3394 1357 Size: 2037 1805 BlpI GC'TnA_GC Cuts at: 954 954 Size: 3842 Bme1580I G_kGCm'C Cuts at: 1467 1560 2616 1467 Size: 93 1056 2693 Fragments arranged by size: 2693 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1054 1135 1470 3614 1054 Size: 81 335 2144 1282 Fragments arranged by size: 2144 1282 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 843 2209 843 Size: 1366 2476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 163 172 251 524 796 163 Size: 9 79 273 272 3209 Fragments arranged by size: 3209 273 272 79 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 247 1018 1897 2389 2687 247 Size: 771 879 492 298 1402 Fragments arranged by size: 1402 879 771 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3546 3546 Size: 3842 BsaAI yAC'GTr Cuts at: 1195 1195 Size: 3842 BsaBI GATnn'nnATC Cuts at: 6 680 1356 3393 6 Size: 674 676 2037 455 Fragments arranged by size: 2037 676 674 455 BsaHI Gr'CG_yC Cuts at: 220 1273 1521 2223 3719 220 Size: 1053 248 702 1496 343 Fragments arranged by size: 1496 1053 702 343 248 BsaWI w'CCGG_w Cuts at: 1031 1552 2230 2504 2651 3062 1031 Size: 521 678 274 147 411 1811 Fragments arranged by size: 1811 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 154 184 154 Size: 30 3812 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 139 139 Size: 3842 BseYI C'CCAG_C Cuts at: 199 1267 2025 2602 3098 199 Size: 1068 758 577 496 943 Fragments arranged by size: 1068 943 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1384 1430 2638 104 Size: 1280 46 1208 1308 Fragments arranged by size: 1308 1280 1208 46 BsiHKAI G_wGCw'C Cuts at: 856 1634 1824 2616 856 Size: 778 190 792 2082 Fragments arranged by size: 2082 792 778 190 Bsp1286I G_dGCh'C Cuts at: 856 1467 1560 1634 1824 1886 2616 856 Size: 611 93 74 190 62 730 2082 Fragments arranged by size: 2082 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3062 3062 Size: 3842 BspHI T'CATG_A Cuts at: 3018 3018 Size: 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 892 1106 1408 1789 892 Size: 214 302 381 2945 Fragments arranged by size: 2945 381 302 214 BsrBI CCG'CTC Cuts at: 50 2134 2188 3672 3837 50 Size: 2084 54 1484 165 55 Fragments arranged by size: 2084 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1754 1754 Size: 3842 BsrFI r'CCGG_y Cuts at: 1840 2021 2230 1840 Size: 181 209 3452 Fragments arranged by size: 3452 209 181 BssSI C'ACGA_G Cuts at: 2113 2471 2113 Size: 358 3484 BstAPI GCAn_nnn'nTGC Cuts at: 1219 1219 Size: 3842 BstBI TT'CG_AA Cuts at: 2203 2203 Size: 3842 BstYI r'GATC_y Cuts at: 1 867 1334 1357 1692 1938 2950 3036 3048 Size: 866 467 23 335 246 1012 86 12 Cuts at: 3048 3394 1 Size: 346 449 Fragments arranged by size: 1012 866 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 531 531 Size: 3842 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1792 1973 2136 1792 Size: 181 163 3498 Fragments arranged by size: 3498 181 163 BtsI GCAGTG_nn' Cuts at: 1085 1085 Size: 3842 DraI TTT'AAA Cuts at: 359 841 359 Size: 482 3360 DrdI GACnn_nn'nnGTC Cuts at: 1548 2406 1548 Size: 858 2984 EaeI y'GGCC_r Cuts at: 1427 1601 1992 2019 3364 1427 Size: 174 391 27 1345 1905 Fragments arranged by size: 1905 1345 391 174 27 EagI C'GGCC_G Cuts at: 1427 1427 Size: 3842 EarI CTCTTCn'nnn_ Cuts at: 465 1865 2075 465 Size: 1400 210 2232 Fragments arranged by size: 2232 1400 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2050 2360 2506 3652 2050 Size: 310 146 1146 2240 Fragments arranged by size: 2240 1146 310 146 EcoICRI GAG'CTC Cuts at: 854 854 Size: 3842 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 163 172 251 401 524 796 1667 2099 2846 Size: 9 79 150 123 272 871 432 747 Cuts at: 2846 3114 163 Size: 268 891 Fragments arranged by size: 891 871 747 432 272 268 150 123 79 9 EcoO109I rG'GnC_Cy Cuts at: 147 981 147 Size: 834 3008 EcoRI G'AATT_C Cuts at: 846 846 Size: 3842 EcoRV GAT'ATC Cuts at: 699 699 Size: 3842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 319 406 1861 319 Size: 87 1455 2300 Fragments arranged by size: 2300 1455 87 FspI TGC'GCA Cuts at: 1623 1623 Size: 3842 HaeII r_GCGC'y Cuts at: 1276 1524 2546 3525 1276 Size: 248 1022 979 1593 Fragments arranged by size: 1593 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 396 428 1684 1714 1716 1746 396 Size: 32 1256 30 2 30 2492 Fragments arranged by size: 2492 1256 32 30 30 2 HincII GTy'rAC Cuts at: 881 881 Size: 3842 KasI G'GCGC_C Cuts at: 1272 1520 1272 Size: 248 3594 KpnI G_GTAC'C Cuts at: 862 862 Size: 3842 MfeI C'AATT_G Cuts at: 548 548 Size: 3842 MluI A'CGCG_T Cuts at: 3275 3275 Size: 3842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2513 2697 2513 Size: 184 3658 MscI TGG'CCA Cuts at: 1603 1603 Size: 3842 MslI CAynn'nnrTG Cuts at: 214 1831 1958 214 Size: 1617 127 2098 Fragments arranged by size: 2098 1617 127 MspA1I CmG'CkG Cuts at: 424 832 953 1267 1627 2640 2885 424 Size: 408 121 314 360 1013 245 1381 Fragments arranged by size: 1381 1013 408 360 314 245 121 NaeI GCC'GGC Cuts at: 2023 2023 Size: 3842 NarI GG'CG_CC Cuts at: 1273 1521 1273 Size: 248 3594 NcoI C'CATG_G Cuts at: 531 531 Size: 3842 NgoMIV G'CCGG_C Cuts at: 2021 2021 Size: 3842 NspI r_CATG'y Cuts at: 895 1395 2302 895 Size: 500 907 2435 Fragments arranged by size: 2435 907 500 PciI A'CATG_T Cuts at: 2298 2298 Size: 3842 PflMI CCAn_nnn'nTGG Cuts at: 708 759 708 Size: 51 3791 PmeI GTTT'AAAC Cuts at: 841 841 Size: 3842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 733 765 2981 3013 733 Size: 32 2216 32 1562 Fragments arranged by size: 2216 1562 32 32 PstI C_TGCA'G Cuts at: 889 1574 889 Size: 685 3157 PvuI CG_AT'CG Cuts at: 104 104 Size: 3842 PvuII CAG'CTG Cuts at: 424 832 1267 1627 424 Size: 408 435 360 2639 Fragments arranged by size: 2639 435 408 360 RsrII CG'GwC_CG Cuts at: 2037 2037 Size: 3842 SacI G_AGCT'C Cuts at: 856 856 Size: 3842 SalI G'TCGA_C Cuts at: 879 879 Size: 3842 SapI GCTCTTCn'nnn_ Cuts at: 1865 2075 1865 Size: 210 3632 SbfI CC_TGCA'GG Cuts at: 889 889 Size: 3842 ScaI AGT'ACT Cuts at: 3359 3359 Size: 3842 SfcI C'TryA_G Cuts at: 33 885 1570 2563 2754 33 Size: 852 685 993 191 1121 Fragments arranged by size: 1121 993 852 685 191 SfoI GGC'GCC Cuts at: 1274 1522 1274 Size: 248 3594 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3842 SmaI CCC'GGG Cuts at: 864 864 Size: 3842 SmlI C'TyrA_G Cuts at: 262 997 1912 2404 2666 262 Size: 735 915 492 262 1438 Fragments arranged by size: 1438 915 735 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3842 SphI G_CATG'C Cuts at: 895 1395 895 Size: 500 3342 SspI AAT'ATT Cuts at: 314 314 Size: 3842 StyI C'CwwG_G Cuts at: 531 976 1330 531 Size: 445 354 3043 Fragments arranged by size: 3043 445 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 213 1425 1558 2017 2226 213 Size: 1212 133 459 209 1829 Fragments arranged by size: 1829 1212 459 209 133 TatI w'GTAC_w Cuts at: 3357 3357 Size: 3842 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 713 745 3359 3391 713 Size: 32 2614 32 1164 Fragments arranged by size: 2614 1164 32 32 Tth111I GACn'n_nGTC Cuts at: 1639 1639 Size: 3842 XbaI T'CTAG_A Cuts at: 330 873 330 Size: 543 3299 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1337 1337 Size: 3842 XmaI C'CCGG_G Cuts at: 862 862 Size: 3842 ZraI GAC'GTC Cuts at: 221 221 Size: 3842 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI