################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII Eam1105I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfuI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp509I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 83 GGTACC G^GTACC AccI 1029 GTCTAC GT^[AC][GT]AC AcyI 286 GGCGCC G[GA]^CG[CT]C AlwNI 1369 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 619 AGCGCT AGC^GCT ApaI 381 GGGCCC GGGCC^C Asp718I 83 GGTACC G^GTACC BbeI 286 GGCGCC GGCGC^C BcgI 404 CGACCACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1529 CTAG C^TAG BlpI 1395 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1395 GCTAAGC GC^T[ACGT]AGC BsaHI 286 GGCGCC G[GA]^CG[CT]C BsaWI 666 TCCGGT [AT]^CCGG[AT] BsiEI 35 CGGCCG CG[GA][CT]^CG BsmI 921 GAATGC GAATGC(1/-1) BspHI 1583 TCATGA T^CATGA BsrBI 1539 CCGCTC CCGCTC(-3/-3) BssSI 1210 CTCGTG CACGAG(-5/-1) BstF5I 269 GGATG GGATG(2/0) CelII 1395 GCTAAGC GC^T[ACGT]AGC DrdI 1288 GACTCCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 35 CGGCCG C^GGCCG EarI 1387 CTCTTC CTCTTC(1/4) EclXI 35 CGGCCG C^GGCCG Eco47III 619 AGCGCT AGC^GCT Eco52I 35 CGGCCG C^GGCCG EcoRV 763 GATATC GAT^ATC FokI 269 GGATG GGATG(9/13) Hin1I 286 GGCGCC G[GA]^CG[CT]C KasI 286 GGCGCC G^GCGCC KpnI 83 GGTACC GGTAC^C Ksp632I 1387 CTCTTC CTCTTC(1/4) MaeI 1529 CTAG C^TAG MseI 1178 TTAA T^TAA NarI 286 GGCGCC GG^CGCC NcoI 866 CCATGG C^CATGG NheI 1528 GCTAGC G^CTAGC NspI 1227 GCATGC [GA]CATG^[CT] RcaI 1583 TCATGA T^CATGA SapI 1386 GCTCTTC GCTCTTC(1/4) SfiI 237 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 286 GGCGCC GGC^GCC SgrAI 288 CGCCGGCG C[GA]^CCGG[CT]G SmlI 718 CTCAAG C^T[CT][GA]AG SphI 1227 GCATGC GCATG^C Tru9I 1178 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AocI 392 CCTCAGG CC^T[ACGT]AGG 496 CCTAAGG AspHI 639 GAGCAC G[AT]GC[AT]^C 715 GTGCTC AspI 835 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1288 GACTCCGTC BamHI 72 GGATCC G^GATCC 324 GGATCC BsiHKAI 639 GAGCAC G[AT]GC[AT]^C 715 GTGCTC BspMI 566 ACCTGC ACCTGC(4/8) 1658 ACCTGC BssHII 54 GCGCGC G^CGCGC 1343 GCGCGC BstYI 72 GGATCC [GA]^GATC[CT] 324 GGATCC Bsu36I 392 CCTCAGG CC^T[ACGT]AGG 496 CCTAAGG DsaI 372 CCACGG C^C[GA][CT]GG 866 CCATGG Eco81I 392 CCTCAGG CC^T[ACGT]AGG 496 CCTAAGG MboII 452 TCTTC GAAGA(8/7) 1388 TCTTC MflI 72 GGATCC [GA]^GATC[CT] 324 GGATCC NaeI 289 GCCGGC GCC^GGC 680 GCCGGC NgoMIV 289 GCCGGC G^CCGGC 680 GCCGGC PflFI 835 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1288 GACTCCGTC PpuMI 892 GGGACCT [GA]G^G[AT]CC[CT] 1715 AGGTCCT PvuII 952 CAGCTG CAG^CTG 1246 CAGCTG TaqI 22 TCGA T^CGA 1391 TCGA TfiI 301 GATTC G^A[AT]TC 770 GAATC Tth111I 835 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1288 GACTCCGTC TthHB8I 22 TCGA T^CGA 1391 TCGA XhoII 72 GGATCC [GA]^GATC[CT] 324 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TseI Tsp45I TspRI XcmI XmaCI XmaI