Restriction map for
pF1KE0322
(Circular) (Six-base) MAPSORT of: pF1KE0322.seq Check: 6022 from: 1 to: 4853
pF1KE0322 4853 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 1138 1891 1138
Size: 753 4100
Acc65I G'GTAC_C
Cuts at: 191 1869 191
Size: 1678 3175
AclI AA'CG_TT
Cuts at: 4693 4693
Size: 4853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1446 1543 2678 3110 3857 4125 1446
Size: 97 1135 432 747 268 2174
Fragments arranged by size:
2174 1135 747 432 268 97
AfeI AGC'GCT
Cuts at: 729 729
Size: 4853
AflIII A'CryG_T
Cuts at: 2205 3309 4286 2205
Size: 1104 977 2772
Fragments arranged by size:
2772 1104 977
AgeI A'CCGG_T
Cuts at: 3241 3241
Size: 4853
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1034 1066 1034
Size: 32 4821
AlwNI CAG_nnn'CTG
Cuts at: 1482 2158 3725 1482
Size: 676 1567 2610
Fragments arranged by size:
2610 1567 676
ApaI G_GGCC'C
Cuts at: 493 493
Size: 4853
ApaLI G'TGCA_C
Cuts at: 3623 3623
Size: 4853
ApoI r'AATT_y
Cuts at: 1857 4206 4462 1857
Size: 2349 256 2248
Fragments arranged by size:
2349 2248 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4853
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
AvaI C'yCGr_G
Cuts at: 297 486 997 1561 1873 297
Size: 189 511 564 312 3277
Fragments arranged by size:
3277 564 511 312 189
BamHI G'GATC_C
Cuts at: 180 432 1878 180
Size: 252 1446 3155
Fragments arranged by size:
3155 1446 252
BanI G'GyrC_C
Cuts at: 191 283 394 648 1869 2283 2531 2566 191
Size: 92 111 254 1221 414 248 35 2478
Fragments arranged by size:
2478 1221 414 254 248 111 92 35
BanII G_rGCy'C
Cuts at: 310 419 493 982 1496 1867 2897 310
Size: 109 74 489 514 371 1030 2266
Fragments arranged by size:
2266 1030 514 489 371 109 74
BbeI G_GCGC'C
Cuts at: 398 2287 2535 398
Size: 1889 248 2716
Fragments arranged by size:
2716 1889 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 501 535 501
Size: 34 4819
BciVI GTATCCnnnnn_n'
Cuts at: 2751 3512 4853 2751
Size: 761 1341 2751
Fragments arranged by size:
2751 1341 761
BclI T'GATC_A
Cuts at: 2373 2373
Size: 4853
BglI GCCn_nnn'nGGC
Cuts at: 352 1384 1477 352
Size: 1032 93 3728
Fragments arranged by size:
3728 1032 93
BglII A'GATC_T
Cuts at: 2368 4405 2368
Size: 2037 2816
BlpI GC'TnA_GC
Cuts at: 1504 1965 1504
Size: 461 4392
Bme1580I G_kGCm'C
Cuts at: 493 2478 2571 3627 493
Size: 1985 93 1056 1719
Fragments arranged by size:
1985 1719 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 111 1184 1647 2065 2146 2481 4625 111
Size: 1073 463 418 81 335 2144 339
Fragments arranged by size:
2144 1073 463 418 339 335 81
BmtI G_CTAG'C
Cuts at: 1640 1640
Size: 4853
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 876 3220 876
Size: 2344 2509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 1057 1070 294
Size: 763 13 4077
Fragments arranged by size:
4077 763 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 2029 2908 3400 3698 811
Size: 1218 879 492 298 1966
Fragments arranged by size:
1966 1218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4557 4557
Size: 4853
BsaAI yAC'GTr
Cuts at: 2206 2206
Size: 4853
BsaBI GATnn'nnATC
Cuts at: 6 2367 4404 6
Size: 2361 2037 455
Fragments arranged by size:
2361 2037 455
BsaHI Gr'CG_yC
Cuts at: 395 2284 2532 3234 4730 395
Size: 1889 248 702 1496 518
Fragments arranged by size:
1889 1496 702 518 248
BsaWI w'CCGG_w
Cuts at: 774 2042 2563 3241 3515 3662 4073 774
Size: 1268 521 678 274 147 411 1554
Fragments arranged by size:
1554 1268 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 681 711 103
Size: 30 548 30 4245
Fragments arranged by size:
4245 548 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 329 834 1378 1381 329
Size: 505 544 3 3801
Fragments arranged by size:
3801 544 505 3
BseYI C'CCAG_C
Cuts at: 1594 2278 3036 3613 4109 1594
Size: 684 758 577 496 2338
Fragments arranged by size:
2338 758 684 577 496
BsiEI CG_ry'CG
Cuts at: 104 146 2395 2441 3649 104
Size: 42 2249 46 1208 1308
Fragments arranged by size:
2249 1308 1208 46 42
BsiHKAI G_wGCw'C
Cuts at: 751 827 1867 2645 2835 3627 751
Size: 76 1040 778 190 792 1977
Fragments arranged by size:
1977 1040 792 778 190 76
BsmI GAATG_Cn'
Cuts at: 1035 1035
Size: 4853
Bsp1286I G_dGCh'C
Cuts at: 310 419 493 751 827 982 1496 1867 2478
Size: 109 74 258 76 155 514 371 611
Cuts at: 2478 2571 2645 2835 2897 3627 310
Size: 93 74 190 62 730 1536
Fragments arranged by size:
1536 730 611 514 371 258 190 155
109 93 76 74 74 62
BspEI T'CCGG_A
Cuts at: 4073 4073
Size: 4853
BspHI T'CATG_A
Cuts at: 1691 4029 1691
Size: 2338 2515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 683 1775 1903 2117 2419 2800 683
Size: 1092 128 214 302 381 2736
Fragments arranged by size:
2736 1092 381 302 214 128
BsrBI CCG'CTC
Cuts at: 50 1649 3145 3199 4683 4848 50
Size: 1599 1496 54 1484 165 55
Fragments arranged by size:
1599 1496 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2765 2765
Size: 4853
BsrFI r'CCGG_y
Cuts at: 188 397 788 2851 3032 3241 188
Size: 209 391 2063 181 209 1800
Fragments arranged by size:
2063 1800 391 209 209 181
BssHII G'CGCG_C
Cuts at: 162 1451 162
Size: 1289 3564
BssSI C'ACGA_G
Cuts at: 1318 3124 3482 1318
Size: 1806 358 2689
Fragments arranged by size:
2689 1806 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2230 2230
Size: 4853
BstBI TT'CG_AA
Cuts at: 3214 3214
Size: 4853
BstYI r'GATC_y
Cuts at: 1 180 432 1878 2345 2368 2703 2949 3961
Size: 179 252 1446 467 23 335 246 1012
Cuts at: 3961 4047 4059 4405 1
Size: 86 12 346 449
Fragments arranged by size:
1446 1012 467 449 346 335 252 246
179 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 501 605 501
Size: 104 4749
BtgI C'CryG_G
Cuts at: 107 480 974 107
Size: 373 494 3986
Fragments arranged by size:
3986 494 373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2803 2984 3147 2803
Size: 181 163 4509
Fragments arranged by size:
4509 181 163
BtsI GCAGTG_nn'
Cuts at: 1710 2096 1710
Size: 386 4467
DraI TTT'AAA
Cuts at: 1852 1852
Size: 4853
DrdI GACnn_nn'nnGTC
Cuts at: 1402 2559 3417 1402
Size: 1157 858 2838
Fragments arranged by size:
2838 1157 858
EaeI y'GGCC_r
Cuts at: 143 344 1752 2438 2612 3003 3030 4375 143
Size: 201 1408 686 174 391 27 1345 621
Fragments arranged by size:
1408 1345 686 621 391 201 174 27
EagI C'GGCC_G
Cuts at: 143 2438 143
Size: 2295 2558
EarI CTCTTCn'nnn_
Cuts at: 1501 2876 3086 1501
Size: 1375 210 3268
Fragments arranged by size:
3268 1375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3061 3371 3517 4663 3061
Size: 310 146 1146 3251
Fragments arranged by size:
3251 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1865 1865
Size: 4853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 1057 1070 1446 1543 2678 3110 3857 4125
Size: 763 13 376 97 1135 432 747 268
Cuts at: 4125 294
Size: 1022
Fragments arranged by size:
1135 1022 763 747 432 376 268 97
13
EcoO109I rG'GnC_Cy
Cuts at: 490 1001 1101 1824 1992 490
Size: 511 100 723 168 3351
Fragments arranged by size:
3351 723 511 168 100
EcoRI G'AATT_C
Cuts at: 1857 1857
Size: 4853
EcoRV GAT'ATC
Cuts at: 873 873
Size: 4853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1170 1321 2872 1170
Size: 151 1551 3151
Fragments arranged by size:
3151 1551 151
FspI TGC'GCA
Cuts at: 2634 2634
Size: 4853
HaeII r_GCGC'y
Cuts at: 202 368 398 731 1374 2287 2535 3557 4536
Size: 166 30 333 643 913 248 1022 979
Cuts at: 4536 202
Size: 519
Fragments arranged by size:
1022 979 913 643 519 333 248 166
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 681 713 2695 2725 2727 2757 681
Size: 32 1982 30 2 30 2777
Fragments arranged by size:
2777 1982 32 30 30 2
HincII GTy'rAC
Cuts at: 1892 1892
Size: 4853
KasI G'GCGC_C
Cuts at: 394 2283 2531 394
Size: 1889 248 2716
Fragments arranged by size:
2716 1889 248
KpnI G_GTAC'C
Cuts at: 195 1873 195
Size: 1678 3175
MluI A'CGCG_T
Cuts at: 4286 4286
Size: 4853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3524 3708 3524
Size: 184 4669
MscI TGG'CCA
Cuts at: 2614 2614
Size: 4853
MslI CAynn'nnrTG
Cuts at: 2842 2969 2842
Size: 127 4726
MspA1I CmG'CkG
Cuts at: 153 1026 1062 1356 1964 2278 2638 3651 3896
Size: 873 36 294 608 314 360 1013 245
Cuts at: 3896 153
Size: 1110
Fragments arranged by size:
1110 1013 873 608 360 314 294 245
36
NaeI GCC'GGC
Cuts at: 399 790 3034 399
Size: 391 2244 2218
Fragments arranged by size:
2244 2218 391
NarI GG'CG_CC
Cuts at: 395 2284 2532 395
Size: 1889 248 2716
Fragments arranged by size:
2716 1889 248
NcoI C'CATG_G
Cuts at: 107 974 107
Size: 867 3986
NgoMIV G'CCGG_C
Cuts at: 397 788 3032 397
Size: 391 2244 2218
Fragments arranged by size:
2244 2218 391
NheI G'CTAG_C
Cuts at: 1636 1636
Size: 4853
NspI r_CATG'y
Cuts at: 1339 1906 2406 3313 1339
Size: 567 500 907 2879
Fragments arranged by size:
2879 907 567 500
PasI CC'CwG_GG
Cuts at: 421 595 421
Size: 174 4679
PciI A'CATG_T
Cuts at: 3309 3309
Size: 4853
PfoI T'CCnGG_A
Cuts at: 1438 1610 1438
Size: 172 4681
PmeI GTTT'AAAC
Cuts at: 1852 1852
Size: 4853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 829 861 3992 4024 829
Size: 32 3131 32 1658
Fragments arranged by size:
3131 1658 32 32
PpuMI rG'GwC_Cy
Cuts at: 1001 1824 1001
Size: 823 4030
PspOMI G'GGCC_C
Cuts at: 489 489
Size: 4853
PstI C_TGCA'G
Cuts at: 466 689 1532 1900 2585 466
Size: 223 843 368 685 2734
Fragments arranged by size:
2734 843 685 368 223
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4853
PvuII CAG'CTG
Cuts at: 1062 1356 2278 2638 1062
Size: 294 922 360 3277
Fragments arranged by size:
3277 922 360 294
RsrII CG'GwC_CG
Cuts at: 3048 3048
Size: 4853
SacI G_AGCT'C
Cuts at: 1867 1867
Size: 4853
SalI G'TCGA_C
Cuts at: 1890 1890
Size: 4853
SapI GCTCTTCn'nnn_
Cuts at: 1501 2876 3086 1501
Size: 1375 210 3268
Fragments arranged by size:
3268 1375 210
SbfI CC_TGCA'GG
Cuts at: 1900 1900
Size: 4853
ScaI AGT'ACT
Cuts at: 4370 4370
Size: 4853
SfcI C'TryA_G
Cuts at: 33 462 685 1528 1896 2581 3574 3765 33
Size: 429 223 843 368 685 993 191 1121
Fragments arranged by size:
1121 993 843 685 429 368 223 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 352 352
Size: 4853
SfoI GGC'GCC
Cuts at: 396 2285 2533 396
Size: 1889 248 2716
Fragments arranged by size:
2716 1889 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
SgrAI Cr'CCGG_yG
Cuts at: 397 397
Size: 4853
SmaI CCC'GGG
Cuts at: 299 488 999 1875 299
Size: 189 511 876 3277
Fragments arranged by size:
3277 876 511 189
SmlI C'TyrA_G
Cuts at: 826 2008 2923 3415 3677 826
Size: 1182 915 492 262 2002
Fragments arranged by size:
2002 1182 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4853
SphI G_CATG'C
Cuts at: 1339 1906 2406 1339
Size: 567 500 3786
Fragments arranged by size:
3786 567 500
SrfI GCCC'GGGC
Cuts at: 299 488 299
Size: 189 4664
StyI C'CwwG_G
Cuts at: 107 974 1094 1428 1987 2341 107
Size: 867 120 334 559 354 2619
Fragments arranged by size:
2619 867 559 354 334 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 493 1189 2436 2569 3028 3237 348
Size: 145 696 1247 133 459 209 1964
Fragments arranged by size:
1964 1247 696 459 209 145 133
TatI w'GTAC_w
Cuts at: 134 221 4368 134
Size: 87 4147 619
Fragments arranged by size:
4147 619 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 1195 1227 4370 4402 103
Size: 32 1060 32 3143 32 554
Fragments arranged by size:
3143 1060 554 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 946 1399 2650 946
Size: 453 1251 3149
Fragments arranged by size:
3149 1251 453
XbaI T'CTAG_A
Cuts at: 1884 1884
Size: 4853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 522 1101 1197 2348 522
Size: 579 96 1151 3027
Fragments arranged by size:
3027 1151 579 96
XmaI C'CCGG_G
Cuts at: 297 486 997 1873 297
Size: 189 511 876 3277
Fragments arranged by size:
3277 876 511 189
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI Bpu10I BsgI BsiWI
BsmBI BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SnaBI SspI StuI SwaI
XhoI XmnI ZraI