Restriction map for pF1KE0322
 (Linear) (Six-base) MAPSORT of: KIEE0322.seq  Check: 6542  from: 1  to: 1740



KIEE0322.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0    1030    1740 
   Size:       1030     710
 
Acc65I G'GTAC_C

Cuts at:      0      83    1740 
   Size:         83    1657
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1338    1435    1740 
   Size:       1338      97     305

  Fragments arranged by size: 

                1338     305      97
 
AfeI AGC'GCT

Cuts at:      0     621    1740 
   Size:        621    1119
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     926     958    1740 
   Size:        926      32     782

  Fragments arranged by size: 

                 926     782      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1374    1740 
   Size:       1374     366
 
ApaI G_GGCC'C

Cuts at:      0     385    1740 
   Size:        385    1355
 
AvaI C'yCGr_G

Cuts at:      0     189     378     889    1453    1740 
   Size:        189     189     511     564     287

  Fragments arranged by size: 

                 564     511     287     189     189
 
BamHI G'GATC_C

Cuts at:      0      72     324    1740 
   Size:         72     252    1416

  Fragments arranged by size: 

                1416     252      72
 
BanI G'GyrC_C

Cuts at:      0      83     175     286     540    1740 
   Size:         83      92     111     254    1200

  Fragments arranged by size: 

                1200     254     111      92      83
 
BanII G_rGCy'C

Cuts at:      0     202     311     385     874    1388    1740 
   Size:        202     109      74     489     514     352

  Fragments arranged by size: 

                 514     489     352     202     109      74
 
BbeI G_GCGC'C

Cuts at:      0     290    1740 
   Size:        290    1450
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     393     427    1740 
   Size:        393      34    1313

  Fragments arranged by size: 

                1313     393      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     244    1276    1369    1740 
   Size:        244    1032      93     371

  Fragments arranged by size: 

                1032     371     244      93
 
BlpI GC'TnA_GC

Cuts at:      0    1396    1740 
   Size:       1396     344
 
Bme1580I G_kGCm'C

Cuts at:      0     385    1740 
   Size:        385    1355
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       3    1076    1539    1740 
   Size:          3    1073     463     201

  Fragments arranged by size: 

                1073     463     201       3
 
BmtI G_CTAG'C

Cuts at:      0    1532    1740 
   Size:       1532     208
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     768    1740 
   Size:        768     972
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     949     962    1740 
   Size:        186     763      13     778

  Fragments arranged by size: 

                 778     763     186      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703    1740 
   Size:        703    1037
 
BsaHI Gr'CG_yC

Cuts at:      0     287    1740 
   Size:        287    1453
 
BsaWI w'CCGG_w

Cuts at:      0     666    1740 
   Size:        666    1074
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     573     603    1740 
   Size:         25     548      30    1137

  Fragments arranged by size: 

                1137     548      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     221     726    1270    1273    1740 
   Size:        221     505     544       3     467

  Fragments arranged by size: 

                 544     505     467     221       3
 
BseYI C'CCAG_C

Cuts at:      0    1486    1740 
   Size:       1486     254
 
BsiEI CG_ry'CG

Cuts at:      0      38    1740 
   Size:         38    1702
 
BsiHKAI G_wGCw'C

Cuts at:      0     643     719    1740 
   Size:        643      76    1021

  Fragments arranged by size: 

                1021     643      76
 
BsmI GAATG_Cn'

Cuts at:      0     927    1740 
   Size:        927     813
 
Bsp1286I G_dGCh'C

Cuts at:      0     202     311     385     643     719     874    1388    1740 
   Size:        202     109      74     258      76     155     514     352

  Fragments arranged by size: 

                 514     352     258     202     155     109      76      74
            
 
BspHI T'CATG_A

Cuts at:      0    1583    1740 
   Size:       1583     157
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     575    1667    1740 
   Size:        575    1092      73

  Fragments arranged by size: 

                1092     575      73
 
BsrBI CCG'CTC

Cuts at:      0    1541    1740 
   Size:       1541     199
 
BsrFI r'CCGG_y

Cuts at:      0      80     289     680    1740 
   Size:         80     209     391    1060

  Fragments arranged by size: 

                1060     391     209      80
 
BssHII G'CGCG_C

Cuts at:      0      54    1343    1740 
   Size:         54    1289     397

  Fragments arranged by size: 

                1289     397      54
 
BssSI C'ACGA_G

Cuts at:      0    1210    1740 
   Size:       1210     530
 
BstYI r'GATC_y

Cuts at:      0      72     324    1740 
   Size:         72     252    1416

  Fragments arranged by size: 

                1416     252      72
 
Bsu36I CC'TnA_GG

Cuts at:      0     393     497    1740 
   Size:        393     104    1243

  Fragments arranged by size: 

                1243     393     104
 
BtgI C'CryG_G

Cuts at:      0     372     866    1740 
   Size:        372     494     874

  Fragments arranged by size: 

                 874     494     372
 
BtsI GCAGTG_nn'

Cuts at:      0    1602    1740 
   Size:       1602     138
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1294    1740 
   Size:       1294     446
 
EaeI y'GGCC_r

Cuts at:      0      35     236    1644    1740 
   Size:         35     201    1408      96

  Fragments arranged by size: 

                1408     201      96      35
 
EagI C'GGCC_G

Cuts at:      0      35    1740 
   Size:         35    1705
 
EarI CTCTTCn'nnn_

Cuts at:      0    1393    1740 
   Size:       1393     347
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     949     962    1338    1435    1740 
   Size:        186     763      13     376      97     305

  Fragments arranged by size: 

                 763     376     305     186      97      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     382     893     993    1716    1740 
   Size:        382     511     100     723      24

  Fragments arranged by size: 

                 723     511     382     100      24
 
EcoRV GAT'ATC

Cuts at:      0     765    1740 
   Size:        765     975
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1062    1213    1740 
   Size:       1062     151     527

  Fragments arranged by size: 

                1062     527     151
 
HaeII r_GCGC'y

Cuts at:      0      94     260     290     623    1266    1740 
   Size:         94     166      30     333     643     474

  Fragments arranged by size: 

                 643     474     333     166      94      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     573     605    1740 
   Size:        573      32    1135

  Fragments arranged by size: 

                1135     573      32
 
KasI G'GCGC_C

Cuts at:      0     286    1740 
   Size:        286    1454
 
KpnI G_GTAC'C

Cuts at:      0      87    1740 
   Size:         87    1653
 
MspA1I CmG'CkG

Cuts at:      0      45     918     954    1248    1740 
   Size:         45     873      36     294     492

  Fragments arranged by size: 

                 873     492     294      45      36
 
NaeI GCC'GGC

Cuts at:      0     291     682    1740 
   Size:        291     391    1058

  Fragments arranged by size: 

                1058     391     291
 
NarI GG'CG_CC

Cuts at:      0     287    1740 
   Size:        287    1453
 
NcoI C'CATG_G

Cuts at:      0     866    1740 
   Size:        866     874
 
NgoMIV G'CCGG_C

Cuts at:      0     289     680    1740 
   Size:        289     391    1060

  Fragments arranged by size: 

                1060     391     289
 
NheI G'CTAG_C

Cuts at:      0    1528    1740 
   Size:       1528     212
 
NspI r_CATG'y

Cuts at:      0    1231    1740 
   Size:       1231     509
 
PasI CC'CwG_GG

Cuts at:      0     313     487    1740 
   Size:        313     174    1253

  Fragments arranged by size: 

                1253     313     174
 
PfoI T'CCnGG_A

Cuts at:      0    1330    1502    1740 
   Size:       1330     172     238

  Fragments arranged by size: 

                1330     238     172
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     721     753    1740 
   Size:        721      32     987

  Fragments arranged by size: 

                 987     721      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     893    1716    1740 
   Size:        893     823      24

  Fragments arranged by size: 

                 893     823      24
 
PspOMI G'GGCC_C

Cuts at:      0     381    1740 
   Size:        381    1359
 
PstI C_TGCA'G

Cuts at:      0     358     581    1424    1740 
   Size:        358     223     843     316

  Fragments arranged by size: 

                 843     358     316     223
 
PvuII CAG'CTG

Cuts at:      0     954    1248    1740 
   Size:        954     294     492

  Fragments arranged by size: 

                 954     492     294
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1393    1740 
   Size:       1393     347
 
SfcI C'TryA_G

Cuts at:      0     354     577    1420    1740 
   Size:        354     223     843     320

  Fragments arranged by size: 

                 843     354     320     223
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     244    1740 
   Size:        244    1496
 
SfoI GGC'GCC

Cuts at:      0     288    1740 
   Size:        288    1452
 
SgrAI Cr'CCGG_yG

Cuts at:      0     289    1740 
   Size:        289    1451
 
SmaI CCC'GGG

Cuts at:      0     191     380     891    1740 
   Size:        191     189     511     849

  Fragments arranged by size: 

                 849     511     191     189
 
SmlI C'TyrA_G

Cuts at:      0     718    1740 
   Size:        718    1022
 
SphI G_CATG'C

Cuts at:      0    1231    1740 
   Size:       1231     509
 
SrfI GCCC'GGGC

Cuts at:      0     191     380    1740 
   Size:        191     189    1360

  Fragments arranged by size: 

                1360     191     189
 
StyI C'CwwG_G

Cuts at:      0     866     986    1320    1740 
   Size:        866     120     334     420

  Fragments arranged by size: 

                 866     420     334     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     240     385    1081    1740 
   Size:        240     145     696     659

  Fragments arranged by size: 

                 696     659     240     145
 
TatI w'GTAC_w

Cuts at:      0      26     113    1740 
   Size:         26      87    1627

  Fragments arranged by size: 

                1627      87      26
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27    1087    1119    1740 
   Size:         27    1060      32     621

  Fragments arranged by size: 

                1060     621      32      27
 
Tth111I GACn'n_nGTC

Cuts at:      0     838    1291    1740 
   Size:        838     453     449

  Fragments arranged by size: 

                 838     453     449
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     414     993    1089    1740 
   Size:        414     579      96     651

  Fragments arranged by size: 

                 651     579     414      96
 
XmaI C'CCGG_G

Cuts at:      0     189     378     889    1740 
   Size:        189     189     511     851

  Fragments arranged by size: 

                 851     511     189     189
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI     AloI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI     BcgI     BglI     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI    BpuEI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrFI   BssHII    BssSI    BstYI   Bsu36I     BtgI     BtsI
     DrdI     EaeI     EagI     EarI  Eco57MI EcoO109I    EcoRV     FalI
    HaeII    Hin4I     KasI     KpnI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NspI     PasI     PfoI     PpiI    PpuMI   PspOMI
     PstI    PvuII     SapI     SfcI     SfiI     SfoI    SgrAI     SmaI
     SmlI     SphI     SrfI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII    BmgBI   Bpu10I
     BsaI    BsaAI    BsaBI     BsgI    BsiWI    BsmBI    BspEI    BsrDI
    BsrGI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     EciI  EcoICRI    EcoNI    EcoRI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     MmeI
     MscI     MslI     NdeI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SgfI    SnaBI     SpeI     SspI     StuI     SwaI     XbaI     XhoI
     XmnI     ZraI
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