(Circular) (Six-base) MAPSORT of: pF1KB0922.seq Check: 9644 from: 1 to: 4148 pF1KB0922 4148 bp With 182 enzymes: SgfI * October 7, 2009 14:14 .. AccI GT'mk_AC Cuts at: 1186 1186 Size: 4148 Acc65I G'GTAC_C Cuts at: 1048 1164 1048 Size: 116 4032 AclI AA'CG_TT Cuts at: 3988 3988 Size: 4148 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1119 1973 2405 3152 3420 1119 Size: 854 432 747 268 1847 Fragments arranged by size: 1847 854 747 432 268 AflIII A'CryG_T Cuts at: 1500 2604 3581 1500 Size: 1104 977 2067 Fragments arranged by size: 2067 1104 977 AgeI A'CCGG_T Cuts at: 2536 2536 Size: 4148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 655 1078 655 Size: 423 3725 AlwNI CAG_nnn'CTG Cuts at: 660 916 1453 3020 660 Size: 256 537 1567 1788 Fragments arranged by size: 1788 1567 537 256 ApaI G_GGCC'C Cuts at: 348 362 1010 348 Size: 14 648 3486 Fragments arranged by size: 3486 648 14 ApaLI G'TGCA_C Cuts at: 253 2918 253 Size: 2665 1483 ApoI r'AATT_y Cuts at: 169 1152 3501 3757 169 Size: 983 2349 256 560 Fragments arranged by size: 2349 983 560 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4148 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4148 AvaI C'yCGr_G Cuts at: 264 751 1168 264 Size: 487 417 3244 Fragments arranged by size: 3244 487 417 BamHI G'GATC_C Cuts at: 1173 1173 Size: 4148 BanI G'GyrC_C Cuts at: 471 1048 1164 1578 1826 1861 471 Size: 577 116 414 248 35 2758 Fragments arranged by size: 2758 577 414 248 116 35 BanII G_rGCy'C Cuts at: 348 362 455 1010 1162 2192 348 Size: 14 93 555 152 1030 2304 Fragments arranged by size: 2304 1030 555 152 93 14 BbeI G_GCGC'C Cuts at: 1582 1830 1582 Size: 248 3900 BbsI GAAGACnn'nnnn_ Cuts at: 787 787 Size: 4148 BciVI GTATCCnnnnn_n' Cuts at: 2046 2807 4148 2046 Size: 761 1341 2046 Fragments arranged by size: 2046 1341 761 BclI T'GATC_A Cuts at: 1668 1668 Size: 4148 BglI GCCn_nnn'nGGC Cuts at: 611 611 Size: 4148 BglII A'GATC_T Cuts at: 1663 3700 1663 Size: 2037 2111 BlpI GC'TnA_GC Cuts at: 926 1260 926 Size: 334 3814 Bme1580I G_kGCm'C Cuts at: 257 348 362 1010 1773 1866 2922 257 Size: 91 14 648 763 93 1056 1483 Fragments arranged by size: 1483 1056 763 648 93 91 14 BmgBI CAC'GTC Cuts at: 276 546 276 Size: 270 3878 BmrI ACTGGGnnnn_n' Cuts at: 977 1360 1441 1776 3920 977 Size: 383 81 335 2144 1205 Fragments arranged by size: 2144 1205 383 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2515 2515 Size: 4148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 387 682 387 Size: 295 3853 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1324 2203 2695 2993 1324 Size: 879 492 298 2479 Fragments arranged by size: 2479 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 556 3852 556 Size: 3296 852 BsaAI yAC'GTr Cuts at: 700 1501 700 Size: 801 3347 BsaBI GATnn'nnATC Cuts at: 6 1662 3699 6 Size: 1656 2037 455 Fragments arranged by size: 2037 1656 455 BsaHI Gr'CG_yC Cuts at: 176 1579 1827 2529 4025 176 Size: 1403 248 702 1496 299 Fragments arranged by size: 1496 1403 702 299 248 BsaWI w'CCGG_w Cuts at: 1337 1858 2536 2810 2957 3368 1337 Size: 521 678 274 147 411 2117 Fragments arranged by size: 2117 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 885 915 885 Size: 30 4118 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1133 1133 Size: 4148 BseYI C'CCAG_C Cuts at: 1016 1100 1573 2331 2908 3404 1016 Size: 84 473 758 577 496 1760 Fragments arranged by size: 1760 758 577 496 473 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 322 403 568 1009 322 Size: 81 165 441 3461 Fragments arranged by size: 3461 441 165 81 BsiEI CG_ry'CG Cuts at: 104 118 1690 1736 2944 104 Size: 14 1572 46 1208 1308 Fragments arranged by size: 1572 1308 1208 46 14 BsiHKAI G_wGCw'C Cuts at: 257 1162 1940 2130 2922 257 Size: 905 778 190 792 1483 Fragments arranged by size: 1483 905 792 778 190 BsmI GAATG_Cn' Cuts at: 1112 1112 Size: 4148 Bsp1286I G_dGCh'C Cuts at: 257 348 362 455 1010 1162 1773 1866 1940 Size: 91 14 93 555 152 611 93 74 Cuts at: 1940 2130 2192 2922 257 Size: 190 62 730 1483 Fragments arranged by size: 1483 730 611 555 190 152 93 93 91 74 62 14 BspEI T'CCGG_A Cuts at: 3368 3368 Size: 4148 BspHI T'CATG_A Cuts at: 3324 3324 Size: 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 317 1031 1198 1412 1714 2095 317 Size: 714 167 214 302 381 2370 Fragments arranged by size: 2370 714 381 302 214 167 BsrBI CCG'CTC Cuts at: 50 218 666 2440 2494 3978 4143 50 Size: 168 448 1774 54 1484 165 55 Fragments arranged by size: 1774 1484 448 168 165 55 54 BsrDI GCAATG_nn' Cuts at: 2060 2060 Size: 4148 BsrFI r'CCGG_y Cuts at: 2146 2327 2536 2146 Size: 181 209 3758 Fragments arranged by size: 3758 209 181 BssHII G'CGCG_C Cuts at: 133 133 Size: 4148 BssSI C'ACGA_G Cuts at: 2419 2777 2419 Size: 358 3790 BstAPI GCAn_nnn'nTGC Cuts at: 1525 1525 Size: 4148 BstBI TT'CG_AA Cuts at: 2509 2509 Size: 4148 BstXI CCAn_nnnn'nTGG Cuts at: 411 411 Size: 4148 BstYI r'GATC_y Cuts at: 1 260 746 1173 1640 1663 1998 2244 3256 Size: 259 486 427 467 23 335 246 1012 Cuts at: 3256 3342 3354 3700 1 Size: 86 12 346 449 Fragments arranged by size: 1012 486 467 449 427 346 335 259 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2098 2279 2442 2098 Size: 181 163 3804 Fragments arranged by size: 3804 181 163 BtsI GCAGTG_nn' Cuts at: 367 957 1391 367 Size: 590 434 3124 Fragments arranged by size: 3124 590 434 DraI TTT'AAA Cuts at: 1147 1147 Size: 4148 DrdI GACnn_nn'nnGTC Cuts at: 1854 2712 1854 Size: 858 3290 EaeI y'GGCC_r Cuts at: 115 350 463 487 1733 1907 2298 2325 3670 Size: 235 113 24 1246 174 391 27 1345 Cuts at: 3670 115 Size: 593 Fragments arranged by size: 1345 1246 593 391 235 174 113 27 24 EagI C'GGCC_G Cuts at: 115 1733 115 Size: 1618 2530 EarI CTCTTCn'nnn_ Cuts at: 157 2171 2381 157 Size: 2014 210 1924 Fragments arranged by size: 2014 1924 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 511 2356 2666 2812 3958 511 Size: 1845 310 146 1146 701 Fragments arranged by size: 1845 1146 701 310 146 EcoICRI GAG'CTC Cuts at: 1160 1160 Size: 4148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 387 682 1119 1973 2405 3152 3420 387 Size: 295 437 854 432 747 268 1115 Fragments arranged by size: 1115 854 747 437 432 295 268 EcoO109I rG'GnC_Cy Cuts at: 345 359 657 898 913 1006 1007 1020 1287 Size: 14 298 241 15 93 1 13 267 Cuts at: 1287 345 Size: 3206 Fragments arranged by size: 3206 298 267 241 93 15 14 13 1 EcoRI G'AATT_C Cuts at: 1152 1152 Size: 4148 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2167 2167 Size: 4148 FspI TGC'GCA Cuts at: 481 1929 481 Size: 1448 2700 HaeII r_GCGC'y Cuts at: 1582 1830 2852 3831 1582 Size: 248 1022 979 1899 Fragments arranged by size: 1899 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 885 917 1990 2020 2022 2052 885 Size: 32 1073 30 2 30 2981 Fragments arranged by size: 2981 1073 32 30 30 2 HincII GTy'rAC Cuts at: 1187 1187 Size: 4148 KasI G'GCGC_C Cuts at: 1578 1826 1578 Size: 248 3900 KpnI G_GTAC'C Cuts at: 1052 1168 1052 Size: 116 4032 MluI A'CGCG_T Cuts at: 3581 3581 Size: 4148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 210 449 2819 3003 210 Size: 239 2370 184 1355 Fragments arranged by size: 2370 1355 239 184 MscI TGG'CCA Cuts at: 352 1909 352 Size: 1557 2591 MslI CAynn'nnrTG Cuts at: 544 590 637 2137 2264 544 Size: 46 47 1500 127 2428 Fragments arranged by size: 2428 1500 127 47 46 MspA1I CmG'CkG Cuts at: 356 407 438 1259 1573 1933 2946 3191 356 Size: 51 31 821 314 360 1013 245 1313 Fragments arranged by size: 1313 1013 821 360 314 245 51 31 NaeI GCC'GGC Cuts at: 2329 2329 Size: 4148 NarI GG'CG_CC Cuts at: 1579 1827 1579 Size: 248 3900 NcoI C'CATG_G Cuts at: 107 107 Size: 4148 NgoMIV G'CCGG_C Cuts at: 2327 2327 Size: 4148 NspI r_CATG'y Cuts at: 1201 1701 2608 1201 Size: 500 907 2741 Fragments arranged by size: 2741 907 500 PasI CC'CwG_GG Cuts at: 609 654 609 Size: 45 4103 PciI A'CATG_T Cuts at: 2604 2604 Size: 4148 PflMI CCAn_nnn'nTGG Cuts at: 660 660 Size: 4148 PfoI T'CCnGG_A Cuts at: 224 672 224 Size: 448 3700 PmeI GTTT'AAAC Cuts at: 1147 1147 Size: 4148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3287 3319 3287 Size: 32 4116 PpuMI rG'GwC_Cy Cuts at: 898 1020 898 Size: 122 4026 PspOMI G'GGCC_C Cuts at: 344 358 1006 344 Size: 14 648 3486 Fragments arranged by size: 3486 648 14 PspXI vC'TCGA_Gb Cuts at: 751 751 Size: 4148 PstI C_TGCA'G Cuts at: 437 912 1195 1880 437 Size: 475 283 685 2705 Fragments arranged by size: 2705 685 475 283 PvuI CG_AT'CG Cuts at: 104 104 Size: 4148 PvuII CAG'CTG Cuts at: 1573 1933 1573 Size: 360 3788 RsrII CG'GwC_CG Cuts at: 2343 2343 Size: 4148 SacI G_AGCT'C Cuts at: 1162 1162 Size: 4148 SalI G'TCGA_C Cuts at: 1185 1185 Size: 4148 SapI GCTCTTCn'nnn_ Cuts at: 157 2171 2381 157 Size: 2014 210 1924 Fragments arranged by size: 2014 1924 210 SbfI CC_TGCA'GG Cuts at: 1195 1195 Size: 4148 ScaI AGT'ACT Cuts at: 3665 3665 Size: 4148 SexAI A'CCwGG_T Cuts at: 956 956 Size: 4148 SfcI C'TryA_G Cuts at: 33 433 908 1191 1876 2869 3060 33 Size: 400 475 283 685 993 191 1121 Fragments arranged by size: 1121 993 685 475 400 283 191 SfoI GGC'GCC Cuts at: 1580 1828 1580 Size: 248 3900 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4148 SmaI CCC'GGG Cuts at: 266 1170 266 Size: 904 3244 SmlI C'TyrA_G Cuts at: 751 1303 2218 2710 2972 751 Size: 552 915 492 262 1927 Fragments arranged by size: 1927 915 552 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4148 SphI G_CATG'C Cuts at: 1201 1701 1201 Size: 500 3648 StyI C'CwwG_G Cuts at: 107 785 1282 1636 107 Size: 678 497 354 2619 Fragments arranged by size: 2619 678 497 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1731 1864 2323 2532 1731 Size: 133 459 209 3347 Fragments arranged by size: 3347 459 209 133 TatI w'GTAC_w Cuts at: 718 3663 718 Size: 2945 1203 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3665 3697 3665 Size: 32 4116 Tth111I GACn'n_nGTC Cuts at: 958 1945 958 Size: 987 3161 XbaI T'CTAG_A Cuts at: 1179 1179 Size: 4148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1643 1643 Size: 4148 XhoI C'TCGA_G Cuts at: 751 751 Size: 4148 XmaI C'CCGG_G Cuts at: 264 1168 264 Size: 904 3244 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI