(Circular) (Six-base) MAPSORT of: pF1KE1596.seq Check: 569 from: 1 to: 4175 pF1KE1596 4175 bp With 182 enzymes: SgfI * January 5, 2010 13:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 755 755 Size: 4175 AccI GT'mk_AC Cuts at: 815 1213 815 Size: 398 3777 Acc65I G'GTAC_C Cuts at: 1191 1191 Size: 4175 AclI AA'CG_TT Cuts at: 4015 4015 Size: 4175 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 915 2000 2432 3179 3447 915 Size: 1085 432 747 268 1643 Fragments arranged by size: 1643 1085 747 432 268 AflIII A'CryG_T Cuts at: 587 1527 2631 3608 587 Size: 940 1104 977 1154 Fragments arranged by size: 1154 1104 977 940 AgeI A'CCGG_T Cuts at: 1058 2563 1058 Size: 1505 2670 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 585 617 585 Size: 32 4143 AlwNI CAG_nnn'CTG Cuts at: 1480 3047 1480 Size: 1567 2608 ApaI G_GGCC'C Cuts at: 138 138 Size: 4175 ApaLI G'TGCA_C Cuts at: 2945 2945 Size: 4175 ApoI r'AATT_y Cuts at: 1179 3528 3784 1179 Size: 2349 256 1570 Fragments arranged by size: 2349 1570 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4175 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4175 AvaI C'yCGr_G Cuts at: 129 945 1195 129 Size: 816 250 3109 Fragments arranged by size: 3109 816 250 BamHI G'GATC_C Cuts at: 1200 1200 Size: 4175 BanI G'GyrC_C Cuts at: 310 733 1191 1605 1853 1888 310 Size: 423 458 414 248 35 2597 Fragments arranged by size: 2597 458 423 414 248 35 BanII G_rGCy'C Cuts at: 138 1189 2219 138 Size: 1051 1030 2094 Fragments arranged by size: 2094 1051 1030 BbeI G_GCGC'C Cuts at: 1609 1857 1609 Size: 248 3927 BbvCI CC'TCA_GC Cuts at: 775 775 Size: 4175 BciVI GTATCCnnnnn_n' Cuts at: 2073 2834 4175 2073 Size: 761 1341 2073 Fragments arranged by size: 2073 1341 761 BclI T'GATC_A Cuts at: 1695 1695 Size: 4175 BfrBI ATG'CAT Cuts at: 892 892 Size: 4175 BglI GCCn_nnn'nGGC Cuts at: 1162 1162 Size: 4175 BglII A'GATC_T Cuts at: 903 1690 3727 903 Size: 787 2037 1351 Fragments arranged by size: 2037 1351 787 BlpI GC'TnA_GC Cuts at: 1007 1287 1007 Size: 280 3895 Bme1580I G_kGCm'C Cuts at: 138 738 1800 1893 2949 138 Size: 600 1062 93 1056 1364 Fragments arranged by size: 1364 1062 1056 600 93 BmrI ACTGGGnnnn_n' Cuts at: 1046 1387 1468 1803 3947 1046 Size: 341 81 335 2144 1274 Fragments arranged by size: 2144 1274 341 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2542 2542 Size: 4175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 784 982 784 Size: 198 3977 Bpu10I CC'TnA_GC Cuts at: 775 820 775 Size: 45 4130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1351 2230 2722 3020 1351 Size: 879 492 298 2506 Fragments arranged by size: 2506 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 565 3879 565 Size: 3314 861 BsaAI yAC'GTr Cuts at: 590 1528 590 Size: 938 3237 BsaBI GATnn'nnATC Cuts at: 6 1689 3726 6 Size: 1683 2037 455 Fragments arranged by size: 2037 1683 455 BsaHI Gr'CG_yC Cuts at: 1146 1606 1854 2556 4052 1146 Size: 460 248 702 1496 1269 Fragments arranged by size: 1496 1269 702 460 248 BsaWI w'CCGG_w Cuts at: 1058 1139 1364 1885 2563 2837 2984 3395 1058 Size: 81 225 521 678 274 147 411 1838 Fragments arranged by size: 1838 678 521 411 274 225 147 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 970 1000 970 Size: 30 4145 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 683 1096 683 Size: 413 3762 BseYI C'CCAG_C Cuts at: 141 605 1600 2358 2935 3431 141 Size: 464 995 758 577 496 885 Fragments arranged by size: 995 885 758 577 496 464 BsiEI CG_ry'CG Cuts at: 104 1717 1763 2971 104 Size: 1613 46 1208 1308 Fragments arranged by size: 1613 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 244 1189 1967 2157 2949 244 Size: 945 778 190 792 1470 Fragments arranged by size: 1470 945 792 778 190 BsmI GAATG_Cn' Cuts at: 894 894 Size: 4175 BsmBI CGTCTCn'nnnn_ Cuts at: 1137 1137 Size: 4175 Bsp1286I G_dGCh'C Cuts at: 138 244 738 1189 1800 1893 1967 2157 2219 Size: 106 494 451 611 93 74 190 62 Cuts at: 2219 2949 138 Size: 730 1364 Fragments arranged by size: 1364 730 611 494 451 190 106 93 74 62 BspEI T'CCGG_A Cuts at: 1139 3395 1139 Size: 2256 1919 BspHI T'CATG_A Cuts at: 3351 3351 Size: 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 755 989 1225 1439 1741 2122 755 Size: 234 236 214 302 381 2808 Fragments arranged by size: 2808 381 302 236 234 214 BsrBI CCG'CTC Cuts at: 50 2467 2521 4005 4170 50 Size: 2417 54 1484 165 55 Fragments arranged by size: 2417 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2087 2087 Size: 4175 BsrFI r'CCGG_y Cuts at: 1058 2173 2354 2563 1058 Size: 1115 181 209 2670 Fragments arranged by size: 2670 1115 209 181 BsrGI T'GTAC_A Cuts at: 650 650 Size: 4175 BssSI C'ACGA_G Cuts at: 2446 2804 2446 Size: 358 3817 BstAPI GCAn_nnn'nTGC Cuts at: 1552 1552 Size: 4175 BstBI TT'CG_AA Cuts at: 2536 2536 Size: 4175 BstXI CCAn_nnnn'nTGG Cuts at: 114 114 Size: 4175 BstYI r'GATC_y Cuts at: 1 903 1200 1667 1690 2025 2271 3283 3369 Size: 902 297 467 23 335 246 1012 86 Cuts at: 3369 3381 3727 1 Size: 12 346 449 Fragments arranged by size: 1012 902 467 449 346 335 297 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 991 991 Size: 4175 BtgI C'CryG_G Cuts at: 227 321 363 464 227 Size: 94 42 101 3938 Fragments arranged by size: 3938 101 94 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2125 2306 2469 2125 Size: 181 163 3831 Fragments arranged by size: 3831 181 163 BtsI GCAGTG_nn' Cuts at: 398 488 1418 398 Size: 90 930 3155 Fragments arranged by size: 3155 930 90 DraI TTT'AAA Cuts at: 1174 1174 Size: 4175 DrdI GACnn_nn'nnGTC Cuts at: 1881 2739 1881 Size: 858 3317 EaeI y'GGCC_r Cuts at: 1760 1934 2325 2352 3697 1760 Size: 174 391 27 1345 2238 Fragments arranged by size: 2238 1345 391 174 27 EagI C'GGCC_G Cuts at: 1760 1760 Size: 4175 EarI CTCTTCn'nnn_ Cuts at: 2198 2408 2198 Size: 210 3965 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1143 2383 2693 2839 3985 1143 Size: 1240 310 146 1146 1333 Fragments arranged by size: 1333 1240 1146 310 146 EcoICRI GAG'CTC Cuts at: 1187 1187 Size: 4175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 784 915 982 2000 2432 3179 3447 784 Size: 131 67 1018 432 747 268 1512 Fragments arranged by size: 1512 1018 747 432 268 131 67 EcoO109I rG'GnC_Cy Cuts at: 134 135 415 795 1164 1314 134 Size: 1 280 380 369 150 2995 Fragments arranged by size: 2995 380 369 280 150 1 EcoRI G'AATT_C Cuts at: 1179 1179 Size: 4175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 4175 FspI TGC'GCA Cuts at: 1956 1956 Size: 4175 HaeII r_GCGC'y Cuts at: 1609 1857 2879 3858 1609 Size: 248 1022 979 1926 Fragments arranged by size: 1926 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 324 356 970 1002 2017 2047 2049 2079 324 Size: 32 614 32 1015 30 2 30 2420 Fragments arranged by size: 2420 1015 614 32 32 30 30 2 HincII GTy'rAC Cuts at: 1214 1214 Size: 4175 KasI G'GCGC_C Cuts at: 1605 1853 1605 Size: 248 3927 KpnI G_GTAC'C Cuts at: 1195 1195 Size: 4175 MluI A'CGCG_T Cuts at: 3608 3608 Size: 4175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 409 499 2846 3030 409 Size: 90 2347 184 1554 Fragments arranged by size: 2347 1554 184 90 MscI TGG'CCA Cuts at: 1936 1936 Size: 4175 MslI CAynn'nnrTG Cuts at: 458 832 2164 2291 458 Size: 374 1332 127 2342 Fragments arranged by size: 2342 1332 374 127 MspA1I CmG'CkG Cuts at: 967 1011 1286 1600 1960 2973 3218 967 Size: 44 275 314 360 1013 245 1924 Fragments arranged by size: 1924 1013 360 314 275 245 44 NaeI GCC'GGC Cuts at: 2356 2356 Size: 4175 NarI GG'CG_CC Cuts at: 1606 1854 1606 Size: 248 3927 NcoI C'CATG_G Cuts at: 363 363 Size: 4175 NgoMIV G'CCGG_C Cuts at: 2354 2354 Size: 4175 NsiI A_TGCA'T Cuts at: 894 894 Size: 4175 NspI r_CATG'y Cuts at: 1228 1728 2635 1228 Size: 500 907 2768 Fragments arranged by size: 2768 907 500 PasI CC'CwG_GG Cuts at: 159 660 918 159 Size: 501 258 3416 Fragments arranged by size: 3416 501 258 PciI A'CATG_T Cuts at: 2631 2631 Size: 4175 PmeI GTTT'AAAC Cuts at: 1174 1174 Size: 4175 PmlI CAC'GTG Cuts at: 590 590 Size: 4175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3314 3346 3314 Size: 32 4143 PpuMI rG'GwC_Cy Cuts at: 415 795 415 Size: 380 3795 PspOMI G'GGCC_C Cuts at: 134 134 Size: 4175 PspXI vC'TCGA_Gb Cuts at: 945 945 Size: 4175 PstI C_TGCA'G Cuts at: 1222 1907 1222 Size: 685 3490 PvuI CG_AT'CG Cuts at: 104 104 Size: 4175 PvuII CAG'CTG Cuts at: 967 1011 1600 1960 967 Size: 44 589 360 3182 Fragments arranged by size: 3182 589 360 44 RsrII CG'GwC_CG Cuts at: 2370 2370 Size: 4175 SacI G_AGCT'C Cuts at: 1189 1189 Size: 4175 SalI G'TCGA_C Cuts at: 1212 1212 Size: 4175 SanDI GG'GwC_CC Cuts at: 415 415 Size: 4175 SapI GCTCTTCn'nnn_ Cuts at: 2198 2408 2198 Size: 210 3965 SbfI CC_TGCA'GG Cuts at: 1222 1222 Size: 4175 ScaI AGT'ACT Cuts at: 3692 3692 Size: 4175 SfcI C'TryA_G Cuts at: 33 1218 1903 2896 3087 33 Size: 1185 685 993 191 1121 Fragments arranged by size: 1185 1121 993 685 191 SfoI GGC'GCC Cuts at: 1607 1855 1607 Size: 248 3927 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4175 SmaI CCC'GGG Cuts at: 1197 1197 Size: 4175 SmlI C'TyrA_G Cuts at: 945 1330 2245 2737 2999 945 Size: 385 915 492 262 2121 Fragments arranged by size: 2121 915 492 385 262 SpeI A'CTAG_T Cuts at: 67 448 67 Size: 381 3794 SphI G_CATG'C Cuts at: 1228 1728 1228 Size: 500 3675 StuI AGG'CCT Cuts at: 676 676 Size: 4175 StyI C'CwwG_G Cuts at: 363 566 710 1309 1663 363 Size: 203 144 599 354 2875 Fragments arranged by size: 2875 599 354 203 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 240 539 1758 1891 2350 2559 240 Size: 299 1219 133 459 209 1856 Fragments arranged by size: 1856 1219 459 299 209 133 TatI w'GTAC_w Cuts at: 650 690 839 3690 650 Size: 40 149 2851 1135 Fragments arranged by size: 2851 1135 149 40 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 366 398 420 452 456 488 953 985 3692 Size: 32 22 32 4 32 465 32 2707 Cuts at: 3692 3724 366 Size: 32 817 Fragments arranged by size: 2707 817 465 32 32 32 32 32 22 4 Tth111I GACn'n_nGTC Cuts at: 1972 1972 Size: 4175 XbaI T'CTAG_A Cuts at: 1206 1206 Size: 4175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1670 1670 Size: 4175 XhoI C'TCGA_G Cuts at: 945 945 Size: 4175 XmaI C'CCGG_G Cuts at: 1195 1195 Size: 4175 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AscI AvrII BaeI BbsI BcgI BmgBI BmtI BsgI BsiWI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PflMI PfoI PshAI PsiI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI