Restriction map for pF1KE1084
 (Circular) (Six-base) MAPSORT of: pF1KE1084.seq  Check: 2638  from: 1  to: 3749



pF1KE1084  3749 bp

 With 182 enzymes: SgfI * 

                        June 30, 2009 14:14  ..

AccI GT'mk_AC

Cuts at:    479     787     479 
   Size:        308    3441
 
Acc65I G'GTAC_C

Cuts at:    765     765 
   Size:       3749
 
AclI AA'CG_TT

Cuts at:   3589    3589 
   Size:       3749
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    239    1574    2006    2753    3021     239 
   Size:       1335     432     747     268     967

  Fragments arranged by size: 

                1335     967     747     432     268
 
AflIII A'CryG_T

Cuts at:    319     335    1101    2205    3182     319 
   Size:         16     766    1104     977     886

  Fragments arranged by size: 

                1104     977     886     766      16
 
AgeI A'CCGG_T

Cuts at:   2137    2137 
   Size:       3749
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    103     135     103 
   Size:         32    3717
 
AlwNI CAG_nnn'CTG

Cuts at:    567     663    1054    2621     567 
   Size:         96     391    1567    1695

  Fragments arranged by size: 

                1695    1567     391      96
 
ApaLI G'TGCA_C

Cuts at:   2519    2519 
   Size:       3749
 
ApoI r'AATT_y

Cuts at:    275     753    3102    3358     275 
   Size:        478    2349     256     666

  Fragments arranged by size: 

                2349     666     478     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3749
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3749
 
AvaI C'yCGr_G

Cuts at:    769     769 
   Size:       3749
 
BamHI G'GATC_C

Cuts at:    774     774 
   Size:       3749
 
BanI G'GyrC_C

Cuts at:    765    1179    1427    1462     765 
   Size:        414     248      35    3052

  Fragments arranged by size: 

                3052     414     248      35
 
BanII G_rGCy'C

Cuts at:    732     763    1793     732 
   Size:         31    1030    2688

  Fragments arranged by size: 

                2688    1030      31
 
BbeI G_GCGC'C

Cuts at:   1183    1431    1183 
   Size:        248    3501
 
BciVI GTATCCnnnnn_n'

Cuts at:   1647    2408    3749    1647 
   Size:        761    1341    1647

  Fragments arranged by size: 

                1647    1341     761
 
BclI T'GATC_A

Cuts at:   1269    1269 
   Size:       3749
 
BglII A'GATC_T

Cuts at:   1264    3301    1264 
   Size:       2037    1712
 
BlpI GC'TnA_GC

Cuts at:    861     861 
   Size:       3749
 
Bme1580I G_kGCm'C

Cuts at:   1374    1467    2523    1374 
   Size:         93    1056    2600

  Fragments arranged by size: 

                2600    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    187     961    1042    1377    3521     187 
   Size:        774      81     335    2144     415

  Fragments arranged by size: 

                2144     774     415     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2116    2116 
   Size:       3749
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    478     478 
   Size:       3749
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    925    1804    2296    2594     925 
   Size:        879     492     298    2080

  Fragments arranged by size: 

                2080     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3453    3453 
   Size:       3749
 
BsaAI yAC'GTr

Cuts at:   1102    1102 
   Size:       3749
 
BsaBI GATnn'nnATC

Cuts at:      6    1263    3300       6 
   Size:       1257    2037     455

  Fragments arranged by size: 

                2037    1257     455
 
BsaHI Gr'CG_yC

Cuts at:   1180    1428    2130    3626    1180 
   Size:        248     702    1496    1303

  Fragments arranged by size: 

                1496    1303     702     248
 
BsaWI w'CCGG_w

Cuts at:    938    1459    2137    2411    2558    2969     938 
   Size:        521     678     274     147     411    1718

  Fragments arranged by size: 

                1718     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    103     133     103 
   Size:         30    3719
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    445     445 
   Size:       3749
 
BseYI C'CCAG_C

Cuts at:   1174    1932    2509    3005    1174 
   Size:        758     577     496    1918

  Fragments arranged by size: 

                1918     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    556     556 
   Size:       3749
 
BsiEI CG_ry'CG

Cuts at:    104    1291    1337    2545     104 
   Size:       1187      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1187      46
 
BsiHKAI G_wGCw'C

Cuts at:    763    1541    1731    2523     763 
   Size:        778     190     792    1989

  Fragments arranged by size: 

                1989     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    732     763    1374    1467    1541    1731    1793    2523     732 
   Size:         31     611      93      74     190      62     730    1958

  Fragments arranged by size: 

                1958     730     611     190      93      74      62      31
            
 
BspEI T'CCGG_A

Cuts at:   2969    2969 
   Size:       3749
 
BspHI T'CATG_A

Cuts at:   2925    2925 
   Size:       3749
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    799    1013    1315    1696     799 
   Size:        214     302     381    2852

  Fragments arranged by size: 

                2852     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2041    2095    3579    3744      50 
   Size:       1991      54    1484     165      55

  Fragments arranged by size: 

                1991    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1661    1661 
   Size:       3749
 
BsrFI r'CCGG_y

Cuts at:   1747    1928    2137    1747 
   Size:        181     209    3359

  Fragments arranged by size: 

                3359     209     181
 
BssSI C'ACGA_G

Cuts at:   2020    2378    2020 
   Size:        358    3391
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1126    1126 
   Size:       3749
 
BstBI TT'CG_AA

Cuts at:   2110    2110 
   Size:       3749
 
BstYI r'GATC_y

Cuts at:      1     774    1241    1264    1599    1845    2857    2943    2955 
   Size:        773     467      23     335     246    1012      86      12

Cuts at:   2955    3301       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     773     467     449     346     335     246      86
                  23      12
 
BstZ17I GTA'TAC

Cuts at:    480     480 
   Size:       3749
 
Bsu36I CC'TnA_GG

Cuts at:    724     724 
   Size:       3749
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1699    1880    2043    1699 
   Size:        181     163    3405

  Fragments arranged by size: 

                3405     181     163
 
BtsI GCAGTG_nn'

Cuts at:    992     992 
   Size:       3749
 
DraI TTT'AAA

Cuts at:    748     748 
   Size:       3749
 
DrdI GACnn_nn'nnGTC

Cuts at:   1455    2313    1455 
   Size:        858    2891
 
EaeI y'GGCC_r

Cuts at:   1334    1508    1899    1926    3271    1334 
   Size:        174     391      27    1345    1812

  Fragments arranged by size: 

                1812    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1334    1334 
   Size:       3749
 
EarI CTCTTCn'nnn_

Cuts at:    258     737    1772    1982     258 
   Size:        479    1035     210    2025

  Fragments arranged by size: 

                2025    1035     479     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1957    2267    2413    3559    1957 
   Size:        310     146    1146    2147

  Fragments arranged by size: 

                2147    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    761     761 
   Size:       3749
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    239     478    1574    2006    2753    3021     239 
   Size:        239    1096     432     747     268     967

  Fragments arranged by size: 

                1096     967     747     432     268     239
 
EcoNI CCTnn'n_nnAGG

Cuts at:    310     310 
   Size:       3749
 
EcoO109I rG'GnC_Cy

Cuts at:    888     888 
   Size:       3749
 
EcoRI G'AATT_C

Cuts at:    753     753 
   Size:       3749
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1768    1768 
   Size:       3749
 
FspI TGC'GCA

Cuts at:    694    1530     694 
   Size:        836    2913
 
HaeII r_GCGC'y

Cuts at:    131    1183    1431    2453    3432     131 
   Size:       1052     248    1022     979     448

  Fragments arranged by size: 

                1052    1022     979     448     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    230     262     283     315     666     698    1591    1621    1623 
   Size:         32      21      32     351      32     893      30       2

Cuts at:   1623    1653     230 
   Size:         30    2326

  Fragments arranged by size: 

                2326     893     351      32      32      32      30      30
                  21       2
 
HincII GTy'rAC

Cuts at:    788     788 
   Size:       3749
 
KasI G'GCGC_C

Cuts at:   1179    1427    1179 
   Size:        248    3501
 
KpnI G_GTAC'C

Cuts at:    769     769 
   Size:       3749
 
MluI A'CGCG_T

Cuts at:   3182    3182 
   Size:       3749
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2420    2604    2420 
   Size:        184    3565
 
MscI TGG'CCA

Cuts at:   1510    1510 
   Size:       3749
 
MslI CAynn'nnrTG

Cuts at:   1738    1865    1738 
   Size:        127    3622
 
MspA1I CmG'CkG

Cuts at:    860    1174    1534    2547    2792     860 
   Size:        314     360    1013     245    1817

  Fragments arranged by size: 

                1817    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1930    1930 
   Size:       3749
 
NarI GG'CG_CC

Cuts at:   1180    1428    1180 
   Size:        248    3501
 
NgoMIV G'CCGG_C

Cuts at:   1928    1928 
   Size:       3749
 
NspI r_CATG'y

Cuts at:    323     339     802    1302    2209     323 
   Size:         16     463     500     907    1863

  Fragments arranged by size: 

                1863     907     500     463      16
 
PasI CC'CwG_GG

Cuts at:    153     153 
   Size:       3749
 
PciI A'CATG_T

Cuts at:    319     335    2205     319 
   Size:         16    1870    1863

  Fragments arranged by size: 

                1870    1863      16
 
PmeI GTTT'AAAC

Cuts at:    748     748 
   Size:       3749
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    103     135    2888    2920     103 
   Size:         32    2753      32     932

  Fragments arranged by size: 

                2753     932      32      32
 
PstI C_TGCA'G

Cuts at:    572     647     668     716     796    1481     572 
   Size:         75      21      48      80     685    2840

  Fragments arranged by size: 

                2840     685      80      75      48      21
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3749
 
PvuII CAG'CTG

Cuts at:   1174    1534    1174 
   Size:        360    3389
 
RsrII CG'GwC_CG

Cuts at:   1944    1944 
   Size:       3749
 
SacI G_AGCT'C

Cuts at:    763     763 
   Size:       3749
 
SalI G'TCGA_C

Cuts at:    786     786 
   Size:       3749
 
SapI GCTCTTCn'nnn_

Cuts at:    737    1772    1982     737 
   Size:       1035     210    2504

  Fragments arranged by size: 

                2504    1035     210
 
SbfI CC_TGCA'GG

Cuts at:    796     796 
   Size:       3749
 
ScaI AGT'ACT

Cuts at:   3266    3266 
   Size:       3749
 
SfcI C'TryA_G

Cuts at:     33     568     643     664     712     792    1477    2470    2661 
   Size:        535      75      21      48      80     685     993     191

Cuts at:   2661      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     535     191      80      75      48
                  21
 
SfoI GGC'GCC

Cuts at:   1181    1429    1181 
   Size:        248    3501
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3749
 
SmaI CCC'GGG

Cuts at:    771     771 
   Size:       3749
 
SmlI C'TyrA_G

Cuts at:    904    1819    2311    2573     904 
   Size:        915     492     262    2080

  Fragments arranged by size: 

                2080     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3749
 
SphI G_CATG'C

Cuts at:    802    1302     802 
   Size:        500    3249
 
StyI C'CwwG_G

Cuts at:    883    1237     883 
   Size:        354    3395
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    124    1332    1465    1924    2133     124 
   Size:       1208     133     459     209    1740

  Fragments arranged by size: 

                1740    1208     459     209     133
 
TatI w'GTAC_w

Cuts at:    544    3264     544 
   Size:       2720    1029
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3266    3298    3266 
   Size:         32    3717
 
Tth111I GACn'n_nGTC

Cuts at:   1546    1546 
   Size:       3749
 
XbaI T'CTAG_A

Cuts at:    588     780     588 
   Size:        192    3557
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1244    1244 
   Size:       3749
 
XmaI C'CCGG_G

Cuts at:    769     769 
   Size:       3749
 
XmnI GAAnn'nnTTC

Cuts at:    115     115 
   Size:       3749
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI     BtgI     ClaI    CspCI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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