(Linear) (Six-base) MAPSORT of: KIEE0666.seq  Check: 9754  from: 1  to: 1071



KIEE0666.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AfeI AGC'GCT

Cuts at:      0     133    1071 
   Size:        133     938
 
AhdI GACnn_n'nnGTC

Cuts at:      0     572    1071 
   Size:        572     499
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      61      93    1071 
   Size:         61      32     978

  Fragments arranged by size: 

                 978      61      32
 
ApoI r'AATT_y

Cuts at:      0     706    1071 
   Size:        706     365
 
AseI AT'TA_AT

Cuts at:      0     841    1071 
   Size:        841     230
 
BanI G'GyrC_C

Cuts at:      0      27     307    1071 
   Size:         27     280     764

  Fragments arranged by size: 

                 764     280      27
 
BanII G_rGCy'C

Cuts at:      0     215    1071 
   Size:        215     856
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     124     216    1071 
   Size:        124      92     855

  Fragments arranged by size: 

                 855     124      92
 
BfrBI ATG'CAT

Cuts at:      0     718    1071 
   Size:        718     353
 
BglII A'GATC_T

Cuts at:      0     858    1071 
   Size:        858     213
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     344    1071 
   Size:        344     727
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     368     823    1071 
   Size:         32     336     455     248

  Fragments arranged by size: 

                 455     336     248      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     301    1071 
   Size:         88     213     770

  Fragments arranged by size: 

                 770     213      88
 
BsaHI Gr'CG_yC

Cuts at:      0     870    1071 
   Size:        870     201
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      61      91    1071 
   Size:         61      30     980

  Fragments arranged by size: 

                 980      61      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82     190     967    1071 
   Size:         82     108     777     104

  Fragments arranged by size: 

                 777     108     104      82
 
BseYI C'CCAG_C

Cuts at:      0     214    1071 
   Size:        214     857
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     500    1071 
   Size:        500     571
 
BsmI GAATG_Cn'

Cuts at:      0     550    1071 
   Size:        550     521
 
Bsp1286I G_dGCh'C

Cuts at:      0     215    1071 
   Size:        215     856
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     756    1071 
   Size:        756     315
 
BsrFI r'CCGG_y

Cuts at:      0      24    1071 
   Size:         24    1047
 
BstBI TT'CG_AA

Cuts at:      0     197    1071 
   Size:        197     874
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     276    1071 
   Size:        276     795
 
BstYI r'GATC_y

Cuts at:      0     858    1071 
   Size:        858     213
 
BtgI C'CryG_G

Cuts at:      0     240     593    1071 
   Size:        240     353     478

  Fragments arranged by size: 

                 478     353     240
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     199     944    1071 
   Size:        148      51     745     127

  Fragments arranged by size: 

                 745     148     127      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     368     823    1071 
   Size:         32     336     455     248

  Fragments arranged by size: 

                 455     336     248      32
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210    1071 
   Size:        210     861
 
HaeII r_GCGC'y

Cuts at:      0       7     135    1071 
   Size:          7     128     936

  Fragments arranged by size: 

                 936     128       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     558     590     703     735     843     875    1071 
   Size:        558      32     113      32     108      32     196

  Fragments arranged by size: 

                 558     196     113     108      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1008    1071 
   Size:       1008      63
 
MslI CAynn'nnrTG

Cuts at:      0     723    1071 
   Size:        723     348
 
NaeI GCC'GGC

Cuts at:      0      26    1071 
   Size:         26    1045
 
NcoI C'CATG_G

Cuts at:      0     593    1071 
   Size:        593     478
 
NgoMIV G'CCGG_C

Cuts at:      0      24    1071 
   Size:         24    1047
 
NsiI A_TGCA'T

Cuts at:      0     720    1071 
   Size:        720     351
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93    1071 
   Size:         61      32     978

  Fragments arranged by size: 

                 978      61      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0     199    1071 
   Size:        199     872
 
SfcI C'TryA_G

Cuts at:      0     476    1071 
   Size:        476     595
 
SmlI C'TyrA_G

Cuts at:      0     103     316    1071 
   Size:        103     213     755

  Fragments arranged by size: 

                 755     213     103
 
StyI C'CwwG_G

Cuts at:      0     593    1071 
   Size:        593     478
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     798    1071 
   Size:        798     273
 

 Enzymes that do cut:

     AfeI     AhdI     AloI     ApoI     AseI     BanI    BanII     BbsI
    BfrBI    BglII     BplI     BpmI    BpuEI    BsaHI    BsaXI    BseRI
    BseYI     BsgI     BsmI Bsp1286I    BspMI    BsrFI    BstBI    BstXI
    BstYI     BtgI     EarI  Eco57MI     FalI    HaeII    Hin4I     MmeI
     MslI     NaeI     NcoI   NgoMIV     NsiI     PpiI     SapI     SfcI
     SmlI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NarI     NdeI
     NheI     NotI     NruI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI