Restriction map for pF1KB5751
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       AclI        AcsI        AflII       AgeI        
AhdI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AviII       AvrII       
BamHI       BbeI        BcgI        BciVI       BfrI        
BglII       BlnI        BsaBI       BsaI        BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstZ17I     Cfr10I      ClaI        
DraI        DrdI        Eam1105I    Eco47III    EcoRI       
EcoRV       EcoT22I     FseI        FspI        HpaI        
KasI        MamI        MfeI        MluI        MslI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        SacI        SalI        
SbfI        SfiI        SfoI        SgrAI       SmlI        
SnaBI       SphI        Sse8387I    SspBI       SwaI        
Tsp509I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         2086  GTAGAC               GT^[AC][GT]AC
AcyI          166  GGCGTC               G[GA]^CG[CT]C
AflIII       1052  ACACGT               A^C[GA][CT]GT
Alw44I        946  GTGCAC               G^TGCAC
ApaI         2039  GGGCCC               GGGCC^C
ApaLI         946  GTGCAC               G^TGCAC
AspI          163  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         943  CACGTG               CAC^GTG
BbsI         1961  GTCTTC               GAAGAC(2/6)
BbvCI        2286  CCTCAGC              CCTCAGC(-5/-2)
BclI         1175  TGATCA               T^GATCA
BglI         1279  GCCATTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1961  GTCTTC               GAAGAC(2/6)
BsaAI         943  CACGTG               [CT]AC^GT[GA]
BsaHI         166  GGCGTC               G[GA]^CG[CT]C
BsiEI         150  CGGCCG               CG[GA][CT]^CG
BstBI        1310  TTCGAA               TT^CGAA
BstYI         920  GGATCT               [GA]^GATC[CT]
CpoI          289  CGGACCG              CG^G[AT]CCG
DraIII       1646  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          150  CGGCCG               C^GGCCG
EarI         1809  GAAGAG               CTCTTC(1/4)
EclXI         150  CGGCCG               C^GGCCG
Eco52I        150  CGGCCG               C^GGCCG
EcoNI         984  CCTGCGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1175  TGATCA               T^GATCA
Hin1I         166  GGCGTC               G[GA]^CG[CT]C
HindIII      1314  AAGCTT               A^AGCTT
Ksp632I      1809  GAAGAG               CTCTTC(1/4)
MflI          920  GGATCT               [GA]^GATC[CT]
MseI          862  TTAA                 T^TAA
NheI          184  GCTAGC               G^CTAGC
NspV         1310  TTCGAA               TT^CGAA
PflFI         163  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         943  CACGTG               CAC^GTG
PmlI          943  CACGTG               CAC^GTG
RsrII         289  CGGACCG              CG^G[AT]CCG
SapI         1809  GAAGAGC              GCTCTTC(1/4)
ScaI          255  AGTACT               AGT^ACT
SexAI        1106  ACCTGGT              A^CC[AT]GGT
SfuI         1310  TTCGAA               TT^CGAA
SmaI          544  CCCGGG               CCC^GGG
SpeI         1423  ACTAGT               A^CTAGT
StuI         1116  AGGCCT               AGG^CCT
Tru9I         862  TTAA                 T^TAA
Tth111I       163  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         920  GGATCT               [GA]^GATC[CT]
XmaCI         544  CCCGGG               C^CCGGG
XmaI          544  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccIII        287  TCCGGA               T^CCGGA
             2153  TCCGGA              
AocI          405  CCTCAGG              CC^T[ACGT]AGG
             2065  CCTCAGG             
BalI          785  TGGCCA               TGG^CCA
             1277  TGGCCA              
BlpI            4  GCTGAGC              GC^T[ACGT]AGC
              836  GCTCAGC             
BpmI         2417  CTCCAG               CTGGAG(16/14)
              901  CTGGAG              
Bpu1102I        4  GCTGAGC              GC^T[ACGT]AGC
              836  GCTCAGC             
BsaWI         287  TCCGGA               [AT]^CCGG[AT]
             2153  TCCGGA              
BseAI         287  TCCGGA               T^CCGGA
             2153  TCCGGA              
BsmBI         727  GAGACG               CGTCTC(1/5)
              994  GAGACG              
BspEI         287  TCCGGA               T^CCGGA
             2153  TCCGGA              
BstEII       1167  GGTCACC              G^GT[ACGT]ACC
             1479  GGTGACC             
BstPI        1167  GGTCACC              G^GT[ACGT]ACC
             1479  GGTGACC             
Bsu36I        405  CCTCAGG              CC^T[ACGT]AGG
             2065  CCTCAGG             
CelII           4  GCTGAGC              GC^T[ACGT]AGC
              836  GCTCAGC             
Eco81I        405  CCTCAGG              CC^T[ACGT]AGG
             2065  CCTCAGG             
EcoO65I      1167  GGTCACC              G^GT[ACGT]ACC
             1479  GGTGACC             
HaeII        1782  GGCGCT               [GA]GCGC^[CT]
             2332  GGCGCT              
HincII       1408  GTCAAC               GT[CT]^[GA]AC
             1659  GTTGAC              
HindII       1408  GTCAAC               GT[CT]^[GA]AC
             1659  GTTGAC              
KspI          207  CCGCGG               CCGC^GG
              551  CCGCGG              
MaeII         944  ACGT                 A^CGT
             1054  ACGT                
MluNI         785  TGGCCA               TGG^CCA
             1277  TGGCCA              
MroI          287  TCCGGA               T^CCGGA
             2153  TCCGGA              
MscI          785  TGGCCA               TGG^CCA
             1277  TGGCCA              
PpuMI         401  GGGTCCT              [GA]G^G[AT]CC[CT]
             1791  GGGACCC             
SacII         207  CCGCGG               CCGC^GG
              551  CCGCGG              
SspI         1611  AATATT               AAT^ATT
             1972  AATATT              
TaqI          855  TCGA                 T^CGA
             1311  TCGA                
TthHB8I       855  TCGA                 T^CGA
             1311  TCGA                
XcmI          890  CCAGTGCAGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2186  CCACCAGTGCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

Acc65I      AccII       AciI        AfaI        AluI        
AlwI        AlwNI       Asp718I     AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BmyI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        KpnI        MaeI        
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        TseI        Tsp45I      TspRI       Van91I      
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