Restriction map for pF1KB5751
 (Linear) (Six-base) MAPSORT of: KIBB5751.seq  Check: 7296  from: 1  to: 2424



KIBB5751.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     430    1233    1908    2424 
   Size:        430     803     675     516

  Fragments arranged by size: 

                 803     675     516     430
 
AccI GT'mk_AC

Cuts at:      0    2087    2424 
   Size:       2087     337
 
Acc65I G'GTAC_C

Cuts at:      0     103    1103    1223    2424 
   Size:        103    1000     120    1201

  Fragments arranged by size: 

                1201    1000     120     103
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99    2424 
   Size:         99    2325
 
AflIII A'CryG_T

Cuts at:      0    1052    2424 
   Size:       1052    1372
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1940    2423    2424 
   Size:       1940     483       1

  Fragments arranged by size: 

                1940     483       1
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1233    1749    1822    1839    1866    2023    2232    2424 
   Size:       1233     516      73      17      27     157     209     192

  Fragments arranged by size: 

                1233     516     209     192     157      73      27      17
            
 
ApaI G_GGCC'C

Cuts at:      0    2043    2424 
   Size:       2043     381
 
ApaLI G'TGCA_C

Cuts at:      0     946    2424 
   Size:        946    1478
 
AvaI C'yCGr_G

Cuts at:      0     544     649    1037    2141    2424 
   Size:        544     105     388    1104     283

  Fragments arranged by size: 

                1104     544     388     283     105
 
BanI G'GyrC_C

Cuts at:      0     103     212     321     442     663    1103    1223    2424 
   Size:        103     109     109     121     221     440     120    1201

  Fragments arranged by size: 

                1201     440     221     121     120     109     109     103
            
 
BanII G_rGCy'C

Cuts at:      0      57     543     724    1600    2043    2424 
   Size:         57     486     181     876     443     381

  Fragments arranged by size: 

                 876     486     443     381     181      57
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1954    2424 
   Size:       1954     470
 
BbvCI CC'TCA_GC

Cuts at:      0    2287    2424 
   Size:       2287     137
 
BclI T'GATC_A

Cuts at:      0    1175    2424 
   Size:       1175    1249
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1285    2424 
   Size:       1285    1139
 
BlpI GC'TnA_GC

Cuts at:      0       5     837    2424 
   Size:          5     832    1587

  Fragments arranged by size: 

                1587     832       5
 
Bme1580I G_kGCm'C

Cuts at:      0     326     447     668     950    2043    2196    2424 
   Size:        326     121     221     282    1093     153     228

  Fragments arranged by size: 

                1093     326     282     228     221     153     121
 
BmgBI CAC'GTC

Cuts at:      0    1055    2424 
   Size:       1055    1369
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     285     496     585    1643    2397    2424 
   Size:        285     211      89    1058     754      27

  Fragments arranged by size: 

                1058     754     285     211      89      27
 
BmtI G_CTAG'C

Cuts at:      0     188    2424 
   Size:        188    2236
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     253     546    1088    2271    2424 
   Size:        253     293     542    1183     153

  Fragments arranged by size: 

                1183     542     293     253     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922    2402    2424 
   Size:        922    1480      22

  Fragments arranged by size: 

                1480     922      22
 
Bpu10I CC'TnA_GC

Cuts at:      0     364    2246    2287    2424 
   Size:        364    1882      41     137

  Fragments arranged by size: 

                1882     364     137      41
 
BsaAI yAC'GTr

Cuts at:      0     945    2424 
   Size:        945    1479
 
BsaHI Gr'CG_yC

Cuts at:      0     167    2424 
   Size:        167    2257
 
BsaWI w'CCGG_w

Cuts at:      0     287    2153    2424 
   Size:        287    1866     271

  Fragments arranged by size: 

                1866     287     271
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     985    1015    2424 
   Size:        985      30    1409

  Fragments arranged by size: 

                1409     985      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     759    1342    1590    2424 
   Size:        759     583     248     834

  Fragments arranged by size: 

                 834     759     583     248
 
BseYI C'CCAG_C

Cuts at:      0      75     246     371     387    2095    2279    2424 
   Size:         75     171     125      16    1708     184     145

  Fragments arranged by size: 

                1708     184     171     145     125      75      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556     914    1261    1894    2419    2424 
   Size:        556     358     347     633     525       5

  Fragments arranged by size: 

                 633     556     525     358     347       5
 
BsiEI CG_ry'CG

Cuts at:      0     153    2424 
   Size:        153    2271
 
BsiHKAI G_wGCw'C

Cuts at:      0     383     950    1816    1901    2424 
   Size:        383     567     866      85     523

  Fragments arranged by size: 

                 866     567     523     383      85
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     721     988    2424 
   Size:        721     267    1436

  Fragments arranged by size: 

                1436     721     267
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     326     383     447     543     668     724     950 
   Size:         57     269      57      64      96     125      56     226

Cuts at:    950    1600    1816    1901    2043    2196    2424 
   Size:        650     216      85     142     153     228

  Fragments arranged by size: 

                 650     269     228     226     216     153     142     125
                  96      85      64      57      57      56
 
BspEI T'CCGG_A

Cuts at:      0     287    2153    2424 
   Size:        287    1866     271

  Fragments arranged by size: 

                1866     287     271
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     430    1233    1908    1916    2424 
   Size:        430     803     675       8     508

  Fragments arranged by size: 

                 803     675     508     430       8
 
BsrBI CCG'CTC

Cuts at:      0     202    2250    2261    2424 
   Size:        202    2048      11     163

  Fragments arranged by size: 

                2048     202     163      11
 
BstBI TT'CG_AA

Cuts at:      0    1311    2424 
   Size:       1311    1113
 
BstEII G'GTnAC_C

Cuts at:      0    1167    1479    2424 
   Size:       1167     312     945

  Fragments arranged by size: 

                1167     945     312
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1212    1275    2196    2424 
   Size:       1212      63     921     228

  Fragments arranged by size: 

                1212     921     228      63
 
BstYI r'GATC_y

Cuts at:      0     920    2424 
   Size:        920    1504
 
Bsu36I CC'TnA_GG

Cuts at:      0     406    2066    2424 
   Size:        406    1660     358

  Fragments arranged by size: 

                1660     406     358
 
BtgI C'CryG_G

Cuts at:      0     207     551     935    1163    2393    2424 
   Size:        207     344     384     228    1230      31

  Fragments arranged by size: 

                1230     384     344     228     207      31
 
BtsI GCAGTG_nn'

Cuts at:      0     337     619     902    1506    1934    2135    2424 
   Size:        337     282     283     604     428     201     289

  Fragments arranged by size: 

                 604     428     337     289     283     282     201
 
DraIII CAC_nnn'GTG

Cuts at:      0    1651    2424 
   Size:       1651     773
 
EaeI y'GGCC_r

Cuts at:      0     150     785    1277    1295    2424 
   Size:        150     635     492      18    1129

  Fragments arranged by size: 

                1129     635     492     150      18
 
EagI C'GGCC_G

Cuts at:      0     150    2424 
   Size:        150    2274
 
EarI CTCTTCn'nnn_

Cuts at:      0    1804    2424 
   Size:       1804     620
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     922    2402    2424 
   Size:         99     823    1480      22

  Fragments arranged by size: 

                1480     823      99      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     988    2424 
   Size:        988    1436
 
EcoO109I rG'GnC_Cy

Cuts at:      0     119     392     402    1680    1792    2039    2424 
   Size:        119     273      10    1278     112     247     385

  Fragments arranged by size: 

                1278     385     273     247     119     112      10
 
HaeII r_GCGC'y

Cuts at:      0    1786    2336    2424 
   Size:       1786     550      88

  Fragments arranged by size: 

                1786     550      88
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     512     544    1039    1068    1071    1100    1276    1308 
   Size:        512      32     495      29       3      29     176      32

Cuts at:   1308    2135    2167    2321    2353    2424 
   Size:        827      32     154      32      71

  Fragments arranged by size: 

                 827     512     495     176     154      71      32      32
                  32      32      29      29       3
 
HincII GTy'rAC

Cuts at:      0    1410    1661    2424 
   Size:       1410     251     763

  Fragments arranged by size: 

                1410     763     251
 
HindIII A'AGCT_T

Cuts at:      0    1314    2424 
   Size:       1314    1110
 
KpnI G_GTAC'C

Cuts at:      0     107    1107    1227    2424 
   Size:        107    1000     120    1197

  Fragments arranged by size: 

                1197    1000     120     107
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1032    1850    2424 
   Size:       1032     818     574

  Fragments arranged by size: 

                1032     818     574
 
MscI TGG'CCA

Cuts at:      0     787    1279    2424 
   Size:        787     492    1145

  Fragments arranged by size: 

                1145     787     492
 
MspA1I CmG'CkG

Cuts at:      0     209     218     371     553     616    1476    1863    2020 
   Size:        209       9     153     182      63     860     387     157

Cuts at:   2020    2106    2424 
   Size:         86     318

  Fragments arranged by size: 

                 860     387     318     209     182     157     153      86
                  63       9
 
NcoI C'CATG_G

Cuts at:      0     935    1163    2393    2424 
   Size:        935     228    1230      31

  Fragments arranged by size: 

                1230     935     228      31
 
NheI G'CTAG_C

Cuts at:      0     184    2424 
   Size:        184    2240
 
PasI CC'CwG_GG

Cuts at:      0     447    2424 
   Size:        447    1977
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1233    1551    1651    2424 
   Size:       1233     318     100     773

  Fragments arranged by size: 

                1233     773     318     100
 
PfoI T'CCnGG_A

Cuts at:      0    1414    2174    2339    2424 
   Size:       1414     760     165      85

  Fragments arranged by size: 

                1414     760     165      85
 
PmlI CAC'GTG

Cuts at:      0     945    2424 
   Size:        945    1479
 
PpuMI rG'GwC_Cy

Cuts at:      0     402    1792    2424 
   Size:        402    1390     632

  Fragments arranged by size: 

                1390     632     402
 
PspOMI G'GGCC_C

Cuts at:      0    2039    2424 
   Size:       2039     385
 
PstI C_TGCA'G

Cuts at:      0    1835    1929    2028    2412    2424 
   Size:       1835      94      99     384      12

  Fragments arranged by size: 

                1835     384      99      94      12
 
PvuII CAG'CTG

Cuts at:      0    1476    1863    2020    2106    2424 
   Size:       1476     387     157      86     318

  Fragments arranged by size: 

                1476     387     318     157      86
 
RsrII CG'GwC_CG

Cuts at:      0     290    2424 
   Size:        290    2134
 
SacII CC_GC'GG

Cuts at:      0     210     554    2424 
   Size:        210     344    1870

  Fragments arranged by size: 

                1870     344     210
 
SanDI GG'GwC_CC

Cuts at:      0    1792    2424 
   Size:       1792     632
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1804    2424 
   Size:       1804     620
 
ScaI AGT'ACT

Cuts at:      0     257    2424 
   Size:        257    2167
 
SexAI A'CCwGG_T

Cuts at:      0    1106    2424 
   Size:       1106    1318
 
SfcI C'TryA_G

Cuts at:      0    1304    1741    1831    1925    2024    2408    2424 
   Size:       1304     437      90      94      99     384      16

  Fragments arranged by size: 

                1304     437     384      99      94      90      16
 
SmaI CCC'GGG

Cuts at:      0     546    2424 
   Size:        546    1878
 
SpeI A'CTAG_T

Cuts at:      0    1423    2424 
   Size:       1423    1001
 
SspI AAT'ATT

Cuts at:      0    1613    1974    2424 
   Size:       1613     361     450

  Fragments arranged by size: 

                1613     450     361
 
StuI AGG'CCT

Cuts at:      0    1118    2424 
   Size:       1118    1306
 
StyI C'CwwG_G

Cuts at:      0     935    1163    2393    2424 
   Size:        935     228    1230      31

  Fragments arranged by size: 

                1230     935     228      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     267     651     901    1558    1662    2293    2424 
   Size:        267     384     250     657     104     631     131

  Fragments arranged by size: 

                 657     631     384     267     250     131     104
 
TatI w'GTAC_w

Cuts at:      0     255    1581    2166    2424 
   Size:        255    1326     585     258

  Fragments arranged by size: 

                1326     585     258     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     680     712    2148    2180    2424 
   Size:        680      32    1436      32     244

  Fragments arranged by size: 

                1436     680     244      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     166    2424 
   Size:        166    2258
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     897    2193    2424 
   Size:        897    1296     231

  Fragments arranged by size: 

                1296     897     231
 
XmaI C'CCGG_G

Cuts at:      0     544    2424 
   Size:        544    1880
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AlfI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI     BclI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EagI
     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII  HindIII
     KpnI     MmeI     MscI   MspA1I     NcoI     NheI     PasI    PflMI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII    RsrII    SacII
    SanDI     SapI     ScaI    SexAI     SfcI     SmaI     SpeI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI    BfrBI    BglII    BpuEI     BsaI    BsaBI    BsiWI
     BsmI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI     HpaI     KasI
     MfeI     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI     SacI     SalI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SphI     SrfI     SwaI
     XbaI     XhoI     XmnI     ZraI
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