Restriction map for pF1KB7984
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        AlwI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DpnII       DraI        DraIII      Eam1105I    
EarI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        Ksp632I     MaeI        
MamI        MboI        MfeI        MflI        MluI        
MroI        MseI        MunI        NaeI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
XbaI        XhoII       


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcsI          780  GAATTC               [GA]^AATT[CT]
AfaI          531  GTAC                 GT^AC
Alw44I        166  GTGCAC               G^TGCAC
AlwNI         313  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         166  GTGCAC               G^TGCAC
ApoI          780  GAATTC               [GA]^AATT[CT]
Asp700I       480  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          863  TGGCCA               TGG^CCA
BbeI          244  GGCGCC               GGCGC^C
BbrPI         846  CACGTG               CAC^GTG
BbvCI         704  CCTCAGC              CCTCAGC(-5/-2)
BsaAI         846  CACGTG               [CT]AC^GT[GA]
BsgI          916  GTGCAG               GTGCAG(16/14)
BsmBI          63  CGTCTC               CGTCTC(1/5)
BsrFI         962  ACCGGC               [GA]^CCGG[CT]
Cfr10I        962  ACCGGC               [GA]^CCGG[CT]
DrdI           48  GACCAAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           59  CGGCCG               C^GGCCG
EclXI          59  CGGCCG               C^GGCCG
Eco52I         59  CGGCCG               C^GGCCG
EcoRI         780  GAATTC               G^AATTC
HaeII         244  GGCGCC               [GA]GCGC^[CT]
HincII        625  GTCAAC               GT[CT]^[GA]AC
HindII        625  GTCAAC               GT[CT]^[GA]AC
HphI           81  TCACC                GGTGA(8/7)
KasI          244  GGCGCC               G^GCGCC
KspI          785  CCGCGG               CCGC^GG
MluNI         863  TGGCCA               TGG^CCA
MscI          863  TGGCCA               TGG^CCA
MslI          678  CATGAGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          244  GGCGCC               GG^CGCC
NcoI          101  CCATGG               C^CATGG
PaeR7I        855  CTCGAG               C^TCGAG
PflMI         312  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         846  CACGTG               CAC^GTG
PmlI          846  CACGTG               CAC^GTG
PpuMI         251  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         714  GACAGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          531  GTAC                 GT^AC
SacII         785  CCGCGG               CCGC^GG
SfoI          244  GGCGCC               GGC^GCC
SmaI          723  CCCGGG               CCC^GGG
SmlI          855  CTCGAG               C^T[CT][GA]AG
TaqI          856  TCGA                 T^CGA
TfiI          423  GAATC                G^A[AT]TC
Tsp509I       781  AATT                 ^AATT
TthHB8I       856  TCGA                 T^CGA
Van91I        312  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          149  CCAAGCACAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          855  CTCGAG               C^TCGAG
XmaCI         723  CCCGGG               C^CCGGG
XmaI          723  CCCGGG               C^CCGGG
XmnI          480  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AspHI          31  GAGCAC               G[AT]GC[AT]^C
              166  GTGCAC              
BanII         505  GGGCTC               G[GA]GC[CT]^C
              726  GGGCTC              
BsiEI          59  CGGCCG               CG[GA][CT]^CG
              133  CGGTCG              
BsiHKAI        31  GAGCAC               G[AT]GC[AT]^C
              166  GTGCAC              
BspLU11I      353  ACATGT               A^CATGT
              527  ACATGT              
BstF5I         85  CATCC                GGATG(2/0)
              575  CATCC               
DraII         251  AGGACCC              [GA]G^G[ACGT]CC[CT]
              566  AGGGCCT             
DsaI          101  CCATGG               C^C[GA][CT]GG
              785  CCGCGG              
EaeI           59  CGGCCG               [CT]^GGCC[GA]
              863  TGGCCA              
EcoO109I      251  AGGACCC              [GA]G^G[ACGT]CC[CT]
              566  AGGGCCT             
EcoT14I       101  CCATGG               C^C[AT][AT]GG
              563  CCAAGG              
FauI           18  CCCGC                CCCGC(4/6)
               43  GCGGG               
FokI           85  CATCC                GGATG(9/13)
              575  CATCC               
HgaI          795  GACGC                GACGC(5/10)
              719  GCGTC               
StyI          101  CCATGG               C^C[AT][AT]GG
              563  CCAAGG              
Tsp45I         80  GTCAC                ^GT[GC]AC
              712  GTGAC               
TspRI         710  CAGTG                CA[GC]TG[ACGT][ACGT]^
              956  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AcyI        AflIII      AluI        
AvaI        AvaII       BanI        BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        EcoRII      
Fnu4HI      HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       ItaI        MaeII       
MaeIII      MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       NspI        PleI        Sau96I      ScrFI       
TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com