Restriction map for pF1KE0908
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# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        AlwI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BsrI        
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        TfiI        Tru9I       Tsp509I     Tth111I     
TthHB8I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   31 enzymes) #
###################################

AcyI          445  GGCGTC               G[GA]^CG[CT]C
Alw44I         45  GTGCAC               G^TGCAC
ApaI          195  GGGCCC               GGGCC^C
ApaLI          45  GTGCAC               G^TGCAC
BanI           59  GGCACC               G^G[CT][GA]CC
BcgI           13  GCAGGGGACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          379  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      379  GCTGAGC              GC^T[ACGT]AGC
BsaHI         445  GGCGTC               G[GA]^CG[CT]C
BsmAI         244  GAGAC                GTCTC(1/5)
BsrDI         438  CATTGC               GCAATG(2/0)
BsrFI         608  ACCGGC               [GA]^CCGG[CT]
BssSI          21  CTCGTG               CACGAG(-5/-1)
CelII         379  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        608  ACCGGC               [GA]^CCGG[CT]
DdeI          380  CTGAG                C^T[ACGT]AG
EaeI          593  CGGCCA               [CT]^GGCC[GA]
EcoT14I       596  CCAAGG               C^C[AT][AT]GG
HaeII         587  GGCGCT               [GA]GCGC^[CT]
HapII         609  CCGG                 C^CGG
HgaI          446  GCGTC                GACGC(5/10)
Hin1I         445  GGCGTC               G[GA]^CG[CT]C
HincII        265  GTTGAC               GT[CT]^[GA]AC
HindII        265  GTTGAC               GT[CT]^[GA]AC
HpaII         609  CCGG                 C^CGG
MspI          609  CCGG                 C^CGG
PvuII         638  CAGCTG               CAG^CTG
SfaNI         624  GATGC                GCATC(5/9)
SmlI          613  CTCAAG               C^T[CT][GA]AG
StuI          323  AGGCCT               AGG^CCT
StyI          596  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BanII         195  GGGCCC               G[GA]GC[CT]^C
              662  GGGCTC              
BglI          416  GCCTCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              425  GCCTGCTGGGC         
BsgI          408  CTGCAC               GTGCAG(16/14)
              652  CTGCAC              
BstF5I        117  CATCC                GGATG(2/0)
               29  GGATG               
CfoI          387  GCGC                 GCG^C
              588  GCGC                
FokI          117  CATCC                GGATG(9/13)
               29  GGATG               
HhaI          387  GCGC                 GCG^C
              588  GCGC                
HinP1I        387  GCGC                 G^CGC
              588  GCGC                
HphI          137  TCACC                GGTGA(8/7)
              647  TCACC               
MspA1I        481  CCGCTG               C[AC]G^C[GT]G
              638  CAGCTG              
NlaIII        450  CATG                 CATG^
              667  CATG                
PflMI         100  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              537  CCAGAAGATGG         
PpuMI          95  GGGACCC              [GA]G^G[AT]CC[CT]
              175  GGGACCT             
Tsp45I        527  GTGAC                ^GT[GC]AC
              646  GTCAC               
Van91I        100  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              537  CCAGAAGATGG         
XcmI          260  CCACTGTTGACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              477  CCAGCCGCTGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       AvaII       
BbvI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BssKI       
BstNI       Cac8I       Cfr13I      DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HinfI       
ItaI        MaeIII      MboII       MnlI        MvaI        
MwoI        NlaIV       PleI        PstI        Sau96I      
ScrFI       SfcI        TseI        TspRI       
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