Restriction map for pF1KE2684
 (Circular) (Six-base) MAPSORT of: pF1KE2684.seq  Check: 8793  from: 1  to: 3764



pF1KE2684  3764 bp

 With 182 enzymes: SgfI * 

                        April 21, 2017 11:06  ..

AccI GT'mk_AC

Cuts at:    653     802     653 
   Size:        149    3615
 
Acc65I G'GTAC_C

Cuts at:    780     780 
   Size:       3764
 
AclI AA'CG_TT

Cuts at:   3604    3604 
   Size:       3764
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1589    2021    2768    3036    1589 
   Size:        432     747     268    2317

  Fragments arranged by size: 

                2317     747     432     268
 
AflIII A'CryG_T

Cuts at:   1116    2220    3197    1116 
   Size:       1104     977    1683

  Fragments arranged by size: 

                1683    1104     977
 
AgeI A'CCGG_T

Cuts at:   2152    2152 
   Size:       3764
 
AleI CACnn'nnGTG

Cuts at:    510     510 
   Size:       3764
 
AlwNI CAG_nnn'CTG

Cuts at:   1069    2636    1069 
   Size:       1567    2197
 
ApaLI G'TGCA_C

Cuts at:   2534    2534 
   Size:       3764
 
ApoI r'AATT_y

Cuts at:    768    3117    3373     768 
   Size:       2349     256    1159

  Fragments arranged by size: 

                2349    1159     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3764
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3764
 
AvaI C'yCGr_G

Cuts at:    784     784 
   Size:       3764
 
BamHI G'GATC_C

Cuts at:    789     789 
   Size:       3764
 
BanI G'GyrC_C

Cuts at:    780    1194    1442    1477     780 
   Size:        414     248      35    3067

  Fragments arranged by size: 

                3067     414     248      35
 
BanII G_rGCy'C

Cuts at:    330     778    1808     330 
   Size:        448    1030    2286

  Fragments arranged by size: 

                2286    1030     448
 
BbeI G_GCGC'C

Cuts at:   1198    1446    1198 
   Size:        248    3516
 
BbsI GAAGACnn'nnnn_

Cuts at:    323     323 
   Size:       3764
 
BciVI GTATCCnnnnn_n'

Cuts at:   1662    2423    3764    1662 
   Size:        761    1341    1662

  Fragments arranged by size: 

                1662    1341     761
 
BclI T'GATC_A

Cuts at:   1284    1284 
   Size:       3764
 
BglII A'GATC_T

Cuts at:   1279    3316    1279 
   Size:       2037    1727
 
BlpI GC'TnA_GC

Cuts at:    876     876 
   Size:       3764
 
Bme1580I G_kGCm'C

Cuts at:    240    1389    1482    2538     240 
   Size:       1149      93    1056    1466

  Fragments arranged by size: 

                1466    1149    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    976    1057    1392    3536     976 
   Size:         81     335    2144    1204

  Fragments arranged by size: 

                2144    1204     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2131    2131 
   Size:       3764
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    940    1819    2311    2609     940 
   Size:        879     492     298    2095

  Fragments arranged by size: 

                2095     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3468    3468 
   Size:       3764
 
BsaAI yAC'GTr

Cuts at:   1117    1117 
   Size:       3764
 
BsaBI GATnn'nnATC

Cuts at:      6    1278    3315       6 
   Size:       1272    2037     455

  Fragments arranged by size: 

                2037    1272     455
 
BsaHI Gr'CG_yC

Cuts at:   1195    1443    2145    3641    1195 
   Size:        248     702    1496    1318

  Fragments arranged by size: 

                1496    1318     702     248
 
BsaWI w'CCGG_w

Cuts at:    123     953    1474    2152    2426    2573    2984     123 
   Size:        830     521     678     274     147     411     903

  Fragments arranged by size: 

                 903     830     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    123     153     123 
   Size:         30    3734
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    178     178 
   Size:       3764
 
BseYI C'CCAG_C

Cuts at:   1189    1947    2524    3020    1189 
   Size:        758     577     496    1933

  Fragments arranged by size: 

                1933     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1306    1352    2560     104 
   Size:       1202      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1202      46
 
BsiHKAI G_wGCw'C

Cuts at:    778    1556    1746    2538     778 
   Size:        778     190     792    2004

  Fragments arranged by size: 

                2004     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    465     465 
   Size:       3764
 
Bsp1286I G_dGCh'C

Cuts at:    240     330     778    1389    1482    1556    1746    1808    2538 
   Size:         90     448     611      93      74     190      62     730

Cuts at:   2538     240 
   Size:       1466

  Fragments arranged by size: 

                1466     730     611     448     190      93      90      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2984    2984 
   Size:       3764
 
BspHI T'CATG_A

Cuts at:   2940    2940 
   Size:       3764
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    293     814    1028    1330    1711     293 
   Size:        521     214     302     381    2346

  Fragments arranged by size: 

                2346     521     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     728    2056    2110    3594    3759      50 
   Size:        678    1328      54    1484     165      55

  Fragments arranged by size: 

                1484    1328     678     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1676    1676 
   Size:       3764
 
BsrFI r'CCGG_y

Cuts at:   1762    1943    2152    1762 
   Size:        181     209    3374

  Fragments arranged by size: 

                3374     209     181
 
BsrGI T'GTAC_A

Cuts at:    332     332 
   Size:       3764
 
BssSI C'ACGA_G

Cuts at:   2035    2393    2035 
   Size:        358    3406
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1141    1141 
   Size:       3764
 
BstBI TT'CG_AA

Cuts at:   2125    2125 
   Size:       3764
 
BstYI r'GATC_y

Cuts at:      1     789    1256    1279    1614    1860    2872    2958    2970 
   Size:        788     467      23     335     246    1012      86      12

Cuts at:   2970    3316       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     788     467     449     346     335     246      86
                  23      12
 
BstZ17I GTA'TAC

Cuts at:    654     654 
   Size:       3764
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3764
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1714    1895    2058    1714 
   Size:        181     163    3420

  Fragments arranged by size: 

                3420     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1007    1007 
   Size:       3764
 
DraI TTT'AAA

Cuts at:    252     763     252 
   Size:        511    3253
 
DrdI GACnn_nn'nnGTC

Cuts at:   1470    2328    1470 
   Size:        858    2906
 
EaeI y'GGCC_r

Cuts at:   1349    1523    1914    1941    3286    1349 
   Size:        174     391      27    1345    1827

  Fragments arranged by size: 

                1827    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1349    1349 
   Size:       3764
 
EarI CTCTTCn'nnn_

Cuts at:    155    1787    1997     155 
   Size:       1632     210    1922

  Fragments arranged by size: 

                1922    1632     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1972    2282    2428    3574    1972 
   Size:        310     146    1146    2162

  Fragments arranged by size: 

                2162    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    776     776 
   Size:       3764
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1589    2021    2768    3036    1589 
   Size:        432     747     268    2317

  Fragments arranged by size: 

                2317     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    903     903 
   Size:       3764
 
EcoRI G'AATT_C

Cuts at:    768     768 
   Size:       3764
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    287    1783     287 
   Size:       1496    2268
 
FspI TGC'GCA

Cuts at:   1545    1545 
   Size:       3764
 
HaeII r_GCGC'y

Cuts at:    563    1198    1446    2468    3447     563 
   Size:        635     248    1022     979     880

  Fragments arranged by size: 

                1022     979     880     635     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    224     256     681     713    1606    1636    1638    1668     224 
   Size:         32     425      32     893      30       2      30    2320

  Fragments arranged by size: 

                2320     893     425      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    803     803 
   Size:       3764
 
KasI G'GCGC_C

Cuts at:   1194    1442    1194 
   Size:        248    3516
 
KpnI G_GTAC'C

Cuts at:    784     784 
   Size:       3764
 
MluI A'CGCG_T

Cuts at:   3197    3197 
   Size:       3764
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2435    2619    2435 
   Size:        184    3580
 
MscI TGG'CCA

Cuts at:   1525    1525 
   Size:       3764
 
MslI CAynn'nnrTG

Cuts at:    510    1753    1880     510 
   Size:       1243     127    2394

  Fragments arranged by size: 

                2394    1243     127
 
MspA1I CmG'CkG

Cuts at:    348     875    1189    1549    2562    2807     348 
   Size:        527     314     360    1013     245    1305

  Fragments arranged by size: 

                1305    1013     527     360     314     245
 
NaeI GCC'GGC

Cuts at:   1945    1945 
   Size:       3764
 
NarI GG'CG_CC

Cuts at:   1195    1443    1195 
   Size:        248    3516
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3764
 
NgoMIV G'CCGG_C

Cuts at:   1943    1943 
   Size:       3764
 
NspI r_CATG'y

Cuts at:    817    1317    2224     817 
   Size:        500     907    2357

  Fragments arranged by size: 

                2357     907     500
 
PciI A'CATG_T

Cuts at:   2220    2220 
   Size:       3764
 
PmeI GTTT'AAAC

Cuts at:    763     763 
   Size:       3764
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2903    2935    2903 
   Size:         32    3732
 
PstI C_TGCA'G

Cuts at:    811    1496     811 
   Size:        685    3079
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3764
 
PvuII CAG'CTG

Cuts at:    348    1189    1549     348 
   Size:        841     360    2563

  Fragments arranged by size: 

                2563     841     360
 
RsrII CG'GwC_CG

Cuts at:   1959    1959 
   Size:       3764
 
SacI G_AGCT'C

Cuts at:    778     778 
   Size:       3764
 
SalI G'TCGA_C

Cuts at:    801     801 
   Size:       3764
 
SapI GCTCTTCn'nnn_

Cuts at:   1787    1997    1787 
   Size:        210    3554
 
SbfI CC_TGCA'GG

Cuts at:    811     811 
   Size:       3764
 
ScaI AGT'ACT

Cuts at:   3281    3281 
   Size:       3764
 
SfcI C'TryA_G

Cuts at:     33     155     223     540     807    1492    2485    2676      33 
   Size:        122      68     317     267     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     317     267     191     122      68
            
 
SfoI GGC'GCC

Cuts at:   1196    1444    1196 
   Size:        248    3516
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3764
 
SmaI CCC'GGG

Cuts at:    786     786 
   Size:       3764
 
SmlI C'TyrA_G

Cuts at:    919    1834    2326    2588     919 
   Size:        915     492     262    2095

  Fragments arranged by size: 

                2095     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3764
 
SphI G_CATG'C

Cuts at:    817    1317     817 
   Size:        500    3264
 
StyI C'CwwG_G

Cuts at:    107     898    1252     107 
   Size:        791     354    2619

  Fragments arranged by size: 

                2619     791     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1347    1480    1939    2148    1347 
   Size:        133     459     209    2963

  Fragments arranged by size: 

                2963     459     209     133
 
TatI w'GTAC_w

Cuts at:    332    3279     332 
   Size:       2947     817
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    123     155    3281    3313     123 
   Size:         32    3126      32     574

  Fragments arranged by size: 

                3126     574      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1561    1561 
   Size:       3764
 
XbaI T'CTAG_A

Cuts at:    795     795 
   Size:       3764
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1259    1259 
   Size:       3764
 
XmaI C'CCGG_G

Cuts at:    784     784 
   Size:       3764
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I     BsgI    BsiWI     BsmI   BssHII   BstEII
    BstXI   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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