Restriction map for pF1KB6957
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstF5I      BstXI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        Ksp632I     KspI        MaeI        MaeII       
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tsp509I     Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I        457  GGTACC               G^GTACC
AcyI          543  GACGCC               G[GA]^CG[CT]C
AlwNI         240  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        65  AGCGCT               AGC^GCT
Asp718I       457  GGTACC               G^GTACC
BanI          457  GGTACC               G^G[CT][GA]CC
BbsI          612  GAAGAC               GAAGAC(2/6)
BpuAI         612  GAAGAC               GAAGAC(2/6)
BsaHI         543  GACGCC               G[GA]^CG[CT]C
BsmAI         404  GTCTC                GTCTC(1/5)
BspHI         407  TCATGA               T^CATGA
BspMI         203  GCAGGT               ACCTGC(4/8)
Eco47III       65  AGCGCT               AGC^GCT
HaeII          65  AGCGCT               [GA]GCGC^[CT]
Hin1I         543  GACGCC               G[GA]^CG[CT]C
HinfI         428  GAATC                G^A[ACGT]TC
KpnI          457  GGTACC               GGTAC^C
MboII         612  GAAGA                GAAGA(8/7)
MseI          319  TTAA                 T^TAA
MslI          395  CACTGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          105  CCATGG               C^CATGG
NspI          285  GCATGT               [GA]CATG^[CT]
PstI          626  CTGCAG               CTGCA^G
RcaI          407  TCATGA               T^CATGA
SacI          373  GAGCTC               GAGCT^C
SfaNI         130  GCATC                GCATC(5/9)
SfiI           16  GGCCAGGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          251  CCCGGG               CCC^GGG
SmlI           28  CTTGAG               C^T[CT][GA]AG
TfiI          428  GAATC                G^A[AT]TC
Tru9I         319  TTAA                 T^TAA
XmaCI         251  CCCGGG               C^CCGGG
XmaI          251  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         220  CGCG                 CG^CG
              519  CGCG                
AfaI          458  GTAC                 GT^AC
              516  GTAC                
AocI           57  CCTCAGG              CC^T[ACGT]AGG
              186  CCTGAGG             
ApaI          143  GGGCCC               GGGCC^C
              150  GGGCCC              
AspHI         373  GAGCTC               G[AT]GC[AT]^C
              600  GAGCAC              
AvaI          251  CCCGGG               C^[CT]CG[GA]G
              547  CCCGAG              
BseRI         189  GAGGAG               GAGGAG(10/8)
              370  GAGGAG              
BsiHKAI       373  GAGCTC               G[AT]GC[AT]^C
              600  GAGCAC              
BsoBI         251  CCCGGG               C^[CT]CG[GA]G
              547  CCCGAG              
BsrFI         176  GCCGGT               [GA]^CCGG[CT]
              194  GCCGGT              
BsrI          232  ACTGG                ACTGG(1/-1)
              396  ACTGG               
BstEII         99  GGTAACC              G^GT[ACGT]ACC
              438  GGTGACC             
BstPI          99  GGTAACC              G^GT[ACGT]ACC
              438  GGTGACC             
BstUI         220  CGCG                 CG^CG
              519  CGCG                
Bsu36I         57  CCTCAGG              CC^T[ACGT]AGG
              186  CCTGAGG             
Cfr10I        176  GCCGGT               [GA]^CCGG[CT]
              194  GCCGGT              
EaeI           92  TGGCCG               [CT]^GGCC[GA]
              521  CGGCCA              
Eco81I         57  CCTCAGG              CC^T[ACGT]AGG
              186  CCTGAGG             
EcoO65I        99  GGTAACC              G^GT[ACGT]ACC
              438  GGTGACC             
EcoT14I       105  CCATGG               C^C[AT][AT]GG
              329  CCAAGG              
HgaI          282  GACGC                GACGC(5/10)
              543  GACGC               
MvnI          220  CGCG                 CG^CG
              519  CGCG                
PflMI         239  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              471  CCAGGGCGTGG         
PpuMI         301  GGGACCC              [GA]G^G[AT]CC[CT]
              559  AGGTCCC             
PvuII         125  CAGCTG               CAG^CTG
              576  CAGCTG              
RsaI          458  GTAC                 GT^AC
              516  GTAC                
SfcI          573  CTACAG               C^T[GA][CT]AG
              626  CTGCAG              
StyI          105  CCATGG               C^C[AT][AT]GG
              329  CCAAGG              
Van91I        239  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              471  CCAGGGCGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BcnI        BglI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EcoO109I    EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeIII      MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       Sau96I      ScrFI       TseI        
Tsp45I      TspRI       
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