(Circular) (Six-base) MAPSORT of: pF1KB5264.seq Check: 7013 from: 1 to: 4049 pF1KB5264 4049 bp With 182 enzymes: SgfI * November 18, 2016 17:23 .. AccI GT'mk_AC Cuts at: 1006 1087 1006 Size: 81 3968 Acc65I G'GTAC_C Cuts at: 1065 1065 Size: 4049 AclI AA'CG_TT Cuts at: 1043 3889 1043 Size: 2846 1203 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1045 1874 2306 3053 3321 1045 Size: 829 432 747 268 1773 Fragments arranged by size: 1773 829 747 432 268 AfeI AGC'GCT Cuts at: 117 117 Size: 4049 AflIII A'CryG_T Cuts at: 1401 2505 3482 1401 Size: 1104 977 1968 Fragments arranged by size: 1968 1104 977 AgeI A'CCGG_T Cuts at: 2437 2437 Size: 4049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 700 700 Size: 4049 AlwNI CAG_nnn'CTG Cuts at: 1354 2921 1354 Size: 1567 2482 ApaLI G'TGCA_C Cuts at: 2819 2819 Size: 4049 ApoI r'AATT_y Cuts at: 397 1053 3402 3658 397 Size: 656 2349 256 788 Fragments arranged by size: 2349 788 656 256 AseI AT'TA_AT Cuts at: 20 587 20 Size: 567 3482 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4049 AvaI C'yCGr_G Cuts at: 246 1069 246 Size: 823 3226 BamHI G'GATC_C Cuts at: 1074 1074 Size: 4049 BanI G'GyrC_C Cuts at: 157 1065 1479 1727 1762 157 Size: 908 414 248 35 2444 Fragments arranged by size: 2444 908 414 248 35 BanII G_rGCy'C Cuts at: 254 946 1017 1063 2093 254 Size: 692 71 46 1030 2210 Fragments arranged by size: 2210 1030 692 71 46 BbeI G_GCGC'C Cuts at: 1483 1731 1483 Size: 248 3801 BciVI GTATCCnnnnn_n' Cuts at: 536 913 1947 2708 4049 536 Size: 377 1034 761 1341 536 Fragments arranged by size: 1341 1034 761 536 377 BclI T'GATC_A Cuts at: 566 1569 566 Size: 1003 3046 BglII A'GATC_T Cuts at: 1564 3601 1564 Size: 2037 2012 BlpI GC'TnA_GC Cuts at: 1161 1161 Size: 4049 Bme1580I G_kGCm'C Cuts at: 160 1674 1767 2823 160 Size: 1514 93 1056 1386 Fragments arranged by size: 1514 1386 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 153 1261 1342 1677 3821 153 Size: 1108 81 335 2144 381 Fragments arranged by size: 2144 1108 381 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 4049 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1225 2104 2596 2894 1225 Size: 879 492 298 2380 Fragments arranged by size: 2380 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3753 3753 Size: 4049 BsaAI yAC'GTr Cuts at: 1402 1402 Size: 4049 BsaBI GATnn'nnATC Cuts at: 6 1563 3600 6 Size: 1557 2037 455 Fragments arranged by size: 2037 1557 455 BsaHI Gr'CG_yC Cuts at: 779 1480 1728 2430 3926 779 Size: 701 248 702 1496 902 Fragments arranged by size: 1496 902 702 701 248 BsaWI w'CCGG_w Cuts at: 833 861 1238 1759 2437 2711 2858 3269 833 Size: 28 377 521 678 274 147 411 1613 Fragments arranged by size: 1613 678 521 411 377 274 147 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 775 775 Size: 4049 BseYI C'CCAG_C Cuts at: 1474 2232 2809 3305 1474 Size: 758 577 496 2218 Fragments arranged by size: 2218 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 808 808 Size: 4049 BsiEI CG_ry'CG Cuts at: 104 1591 1637 2845 104 Size: 1487 46 1208 1308 Fragments arranged by size: 1487 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1063 1841 2031 2823 1063 Size: 778 190 792 2289 Fragments arranged by size: 2289 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 859 859 Size: 4049 Bsp1286I G_dGCh'C Cuts at: 160 254 946 1017 1063 1674 1767 1841 2031 Size: 94 692 71 46 611 93 74 190 Cuts at: 2031 2093 2823 160 Size: 62 730 1386 Fragments arranged by size: 1386 730 692 611 190 94 93 74 71 62 46 BspEI T'CCGG_A Cuts at: 833 861 3269 833 Size: 28 2408 1613 Fragments arranged by size: 2408 1613 28 BspHI T'CATG_A Cuts at: 989 3225 989 Size: 2236 1813 BspMI ACCTGCnnnn'nnnn_ Cuts at: 968 1099 1313 1615 1996 968 Size: 131 214 302 381 3021 Fragments arranged by size: 3021 381 302 214 131 BsrBI CCG'CTC Cuts at: 50 2341 2395 3879 4044 50 Size: 2291 54 1484 165 55 Fragments arranged by size: 2291 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 419 1961 419 Size: 1542 2507 BsrFI r'CCGG_y Cuts at: 130 2047 2228 2437 130 Size: 1917 181 209 1742 Fragments arranged by size: 1917 1742 209 181 BssSI C'ACGA_G Cuts at: 2320 2678 2320 Size: 358 3691 BstAPI GCAn_nnn'nTGC Cuts at: 1426 1426 Size: 4049 BstBI TT'CG_AA Cuts at: 2410 2410 Size: 4049 BstYI r'GATC_y Cuts at: 1 643 1074 1541 1564 1899 2145 3157 3243 Size: 642 431 467 23 335 246 1012 86 Cuts at: 3243 3255 3601 1 Size: 12 346 449 Fragments arranged by size: 1012 642 467 449 431 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 161 107 Size: 54 3995 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1999 2180 2343 1999 Size: 181 163 3705 Fragments arranged by size: 3705 181 163 BtsI GCAGTG_nn' Cuts at: 684 1292 684 Size: 608 3441 DraI TTT'AAA Cuts at: 1048 1048 Size: 4049 DrdI GACnn_nn'nnGTC Cuts at: 1755 2613 1755 Size: 858 3191 EaeI y'GGCC_r Cuts at: 1634 1808 2199 2226 3571 1634 Size: 174 391 27 1345 2112 Fragments arranged by size: 2112 1345 391 174 27 EagI C'GGCC_G Cuts at: 1634 1634 Size: 4049 EarI CTCTTCn'nnn_ Cuts at: 202 305 2072 2282 202 Size: 103 1767 210 1969 Fragments arranged by size: 1969 1767 210 103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2257 2567 2713 3859 2257 Size: 310 146 1146 2447 Fragments arranged by size: 2447 1146 310 146 EcoICRI GAG'CTC Cuts at: 1061 1061 Size: 4049 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1045 1874 2306 3053 3321 1045 Size: 829 432 747 268 1773 Fragments arranged by size: 1773 829 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 757 757 Size: 4049 EcoO109I rG'GnC_Cy Cuts at: 1188 1188 Size: 4049 EcoRI G'AATT_C Cuts at: 1053 1053 Size: 4049 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 798 2068 798 Size: 1270 2779 FspI TGC'GCA Cuts at: 1830 1830 Size: 4049 HaeII r_GCGC'y Cuts at: 119 1483 1731 2753 3732 119 Size: 1364 248 1022 979 436 Fragments arranged by size: 1364 1022 979 436 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 421 453 727 759 841 873 911 918 943 Size: 32 274 32 82 32 38 7 25 Cuts at: 943 950 1891 1921 1923 1953 421 Size: 7 941 30 2 30 2517 Fragments arranged by size: 2517 941 274 82 38 32 32 32 30 30 25 7 7 2 HincII GTy'rAC Cuts at: 429 1088 429 Size: 659 3390 KasI G'GCGC_C Cuts at: 1479 1727 1479 Size: 248 3801 KpnI G_GTAC'C Cuts at: 1069 1069 Size: 4049 MfeI C'AATT_G Cuts at: 616 616 Size: 4049 MluI A'CGCG_T Cuts at: 3482 3482 Size: 4049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2720 2904 2720 Size: 184 3865 MscI TGG'CCA Cuts at: 1810 1810 Size: 4049 MslI CAynn'nnrTG Cuts at: 496 994 2038 2165 496 Size: 498 1044 127 2380 Fragments arranged by size: 2380 1044 498 127 MspA1I CmG'CkG Cuts at: 1160 1474 1834 2847 3092 1160 Size: 314 360 1013 245 2117 Fragments arranged by size: 2117 1013 360 314 245 NaeI GCC'GGC Cuts at: 132 2230 132 Size: 2098 1951 NarI GG'CG_CC Cuts at: 1480 1728 1480 Size: 248 3801 NcoI C'CATG_G Cuts at: 107 107 Size: 4049 NdeI CA'TA_TG Cuts at: 303 303 Size: 4049 NgoMIV G'CCGG_C Cuts at: 130 2228 130 Size: 2098 1951 NspI r_CATG'y Cuts at: 687 1102 1602 2509 687 Size: 415 500 907 2227 Fragments arranged by size: 2227 907 500 415 PciI A'CATG_T Cuts at: 2505 2505 Size: 4049 PmeI GTTT'AAAC Cuts at: 1048 1048 Size: 4049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3188 3220 3188 Size: 32 4017 PstI C_TGCA'G Cuts at: 1096 1781 1096 Size: 685 3364 PvuI CG_AT'CG Cuts at: 104 104 Size: 4049 PvuII CAG'CTG Cuts at: 1474 1834 1474 Size: 360 3689 RsrII CG'GwC_CG Cuts at: 2244 2244 Size: 4049 SacI G_AGCT'C Cuts at: 1063 1063 Size: 4049 SalI G'TCGA_C Cuts at: 1086 1086 Size: 4049 SapI GCTCTTCn'nnn_ Cuts at: 202 2072 2282 202 Size: 1870 210 1969 Fragments arranged by size: 1969 1870 210 SbfI CC_TGCA'GG Cuts at: 1096 1096 Size: 4049 ScaI AGT'ACT Cuts at: 282 3566 282 Size: 3284 765 SexAI A'CCwGG_T Cuts at: 371 371 Size: 4049 SfcI C'TryA_G Cuts at: 33 1092 1777 2770 2961 33 Size: 1059 685 993 191 1121 Fragments arranged by size: 1121 1059 993 685 191 SfoI GGC'GCC Cuts at: 1481 1729 1481 Size: 248 3801 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4049 SmaI CCC'GGG Cuts at: 248 1071 248 Size: 823 3226 SmlI C'TyrA_G Cuts at: 1204 2119 2611 2873 1204 Size: 915 492 262 2380 Fragments arranged by size: 2380 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4049 SphI G_CATG'C Cuts at: 687 1102 1602 687 Size: 415 500 3134 Fragments arranged by size: 3134 500 415 SspI AAT'ATT Cuts at: 585 591 585 Size: 6 4043 StyI C'CwwG_G Cuts at: 107 1183 1537 107 Size: 1076 354 2619 Fragments arranged by size: 2619 1076 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1632 1765 2224 2433 1632 Size: 133 459 209 3248 Fragments arranged by size: 3248 459 209 133 TatI w'GTAC_w Cuts at: 140 280 3564 140 Size: 140 3284 625 Fragments arranged by size: 3284 625 140 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3566 3598 3566 Size: 32 4017 Tth111I GACn'n_nGTC Cuts at: 1846 1846 Size: 4049 XbaI T'CTAG_A Cuts at: 1080 1080 Size: 4049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1544 1544 Size: 4049 XmaI C'CCGG_G Cuts at: 246 1069 246 Size: 823 3226 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI