Restriction map for pF1KB7983
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AhdI        Alw44I      
AlwI        AlwNI       AocI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BseAI       BsgI        BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          883  GTCTAC               GT^[AC][GT]AC
AfaI          335  GTAC                 GT^AC
AgeI          912  ACCGGT               A^CCGGT
Aor51HI       979  AGCGCT               AGC^GCT
ApaI          107  GGGCCC               GGGCC^C
BalI         1252  TGGCCA               TGG^CCA
BanII         107  GGGCCC               G[GA]GC[CT]^C
BbsI          824  GAAGAC               GAAGAC(2/6)
BciVI         486  GTATCC               GTATCC(6/5)
BglII         741  AGATCT               A^GATCT
BpuAI         824  GAAGAC               GAAGAC(2/6)
BseRI        1053  CTCCTC               GAGGAG(10/8)
BsiEI         112  CGGCCG               CG[GA][CT]^CG
BsrFI         912  ACCGGT               [GA]^CCGG[CT]
BstYI         741  AGATCT               [GA]^GATC[CT]
Cfr10I        912  ACCGGT               [GA]^CCGG[CT]
DpnII         742  GATC                 ^GATC
DsaI         1007  CCATGG               C^C[GA][CT]GG
EagI          112  CGGCCG               C^GGCCG
EarI          797  GAAGAG               CTCTTC(1/4)
EclXI         112  CGGCCG               C^GGCCG
Eco47III      979  AGCGCT               AGC^GCT
Eco52I        112  CGGCCG               C^GGCCG
EcoRI         131  GAATTC               G^AATTC
EcoT14I      1007  CCATGG               C^C[AT][AT]GG
HaeII         979  AGCGCT               [GA]GCGC^[CT]
HincII         46  GTCAAC               GT[CT]^[GA]AC
HindII         46  GTCAAC               GT[CT]^[GA]AC
Ksp632I       797  GAAGAG               CTCTTC(1/4)
MaeIII         11  GTCAC                ^GT[ACGT]AC
MboI          742  GATC                 ^GATC
MflI          741  AGATCT               [GA]^GATC[CT]
MluNI        1252  TGGCCA               TGG^CCA
MscI         1252  TGGCCA               TGG^CCA
NcoI         1007  CCATGG               C^CATGG
NdeI         1126  CATATG               CA^TATG
NdeII         742  GATC                 ^GATC
PinAI         912  ACCGGT               A^CCGGT
PstI          136  CTGCAG               CTGCA^G
PvuII         613  CAGCTG               CAG^CTG
RsaI          335  GTAC                 GT^AC
Sau3AI        742  GATC                 ^GATC
SphI         1067  GCATGC               GCATG^C
StyI         1007  CCATGG               C^C[AT][AT]GG
TaqI          780  TCGA                 T^CGA
Tsp45I         11  GTCAC                ^GT[GC]AC
TthHB8I       780  TCGA                 T^CGA
XcmI          998  CCAGCTTCACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         741  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          131  GAATTC               [GA]^AATT[CT]
              164  AAATTC              
ApoI          131  GAATTC               [GA]^AATT[CT]
              164  AAATTC              
AvaI          716  CCCGAG               C^[CT]CG[GA]G
             1164  CTCGGG              
BpmI          239  CTCCAG               CTGGAG(16/14)
              673  CTGGAG              
BsaWI         457  ACCGGA               [AT]^CCGG[AT]
              912  ACCGGT              
BsiYI         458  CCGGAAGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              531  CCAGCAACAGG         
BslI          458  CCGGAAGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              531  CCAGCAACAGG         
BsmFI         493  GGGAC                GGGAC(10/14)
             1039  GTCCC               
BsoBI         716  CCCGAG               C^[CT]CG[GA]G
             1164  CTCGGG              
CfoI          413  GCGC                 GCG^C
              980  GCGC                
DdeI          436  CTAAG                C^T[ACGT]AG
              811  CTGAG               
DraII         106  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              668  AGGCCCT             
EcoO109I      106  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              668  AGGCCCT             
HhaI          413  GCGC                 GCG^C
              980  GCGC                
HinP1I        413  GCGC                 G^CGC
              980  GCGC                
HphI         1089  GGTGA                GGTGA(8/7)
             1004  TCACC               
NheI           93  GCTAGC               G^CTAGC
              391  GCTAGC              
NspI          422  GCATGT               [GA]CATG^[CT]
             1067  GCATGC              
SfcI          136  CTGCAG               C^T[GA][CT]AG
              975  CTACAG              
TspRI          59  CACTG                CA[GC]TG[ACGT][ACGT]^
              879  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        BanI        BbvI        BcnI        
BfaI        BmyI        BsaJI       BsmAI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        MaeI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       PleI        Sau96I      ScrFI       
TfiI        TseI        Tsp509I     
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